NO对杨梅采后活性氧代谢和腐烂的影响

doi:10.11707/j.1001-7488.20101211

林业科学 ›› 2010, Vol. 46 ›› Issue (12): 70-74.doi: 10.11707/j.1001-7488.20101211

NO对杨梅采后活性氧代谢和腐烂的影响

杨虎清, 吴峰华, 周存山, 王允祥

浙江农林大学农业与食品科学学院临安311300

收稿日期:2009-10-20 修回日期:2010-01-06 出版日期:2010-12-25 发布日期:2010-12-25
通讯作者: 王允祥

Effects of Nitric Oxide on Active Oxygen Metabolism and Fruit Decay in Postharvest Chinese Bayberry

Yang Huqing, Wu Fenghua, Zhou Cunshan, Wang Yunxiang

School of Agriculture and Food Science,Zhejiang A & F University Lin'an 311300

Received:2009-10-20 Revised:2010-01-06 Online:2010-12-25 Published:2010-12-25

摘要/Abstract

摘要：

用0,10,25和50 μmol ·L^-1硝普钠(SNP)浸泡杨梅2 h,在(0±0.5)℃贮藏16天。贮藏期间每隔4天取样测定活性氧产量、相对电导率、MDA含量及相关酶类如SOD,CAT,POD 的变化,最后统计腐烂指数。结果显示: 25 μmol ·L^-1 SNP 释放的NO处理可降低杨梅果实的腐烂率,延缓V_c 的降解,抑制贮藏前期可滴定酸的下降和总糖的变化,提高杨梅果实中SOD,CAT,POD 保护酶的活性,抑制H₂O₂,O^·₂含量的增加,降低MDA的含量和膜透性的升高,防止膜脂过氧化,延缓果实的衰老。适宜浓度的NO处理可维持杨梅采后活性氧代谢的平衡,从而延缓果实的衰老进程和抑制果实腐烂,保持杨梅良好的贮藏品质。

关键词: 杨梅, 一氧化氮, 活性氧代谢, 腐烂, 生理指标

Abstract:

Influences of NO on active oxygen metabolism and relevant quality of Chinese Bayberry (Myrica rubra) fruits were studied to provide scientific basis of NO application in fresh preservation. Fruits of Chinese Bayberry were immersed with 0,10,25,and 50 μmol ·L^-1 sodium nitroprusside(NO donor) respectively for 2 hours,and then stored for 16 d at (0±0.5)℃. Active oxygen production,relative electric conductivity,MDA content, enzymatic activity of SOD,CAT and POD and decay index were measured thereafter in every 4 days. The results showed that NO from 25 μmol ·L^-1 SNP significantly decreased the decay of Chinese Bayberry and inhibited the decline of vitamin C,titratable acid,and total sugar compared with the control fruits. NO treatment maintained significantly higher levels of SOD,CAT and POD activities of Chinese Bayberry,and inhibited the increase of H₂O₂,superoxide radical production,relative electric conductivity and MDA content. This study suggests that NO could maintain the balance of active oxygen metabolism in Chinese Bayberry,and thereby delaying fruit senescence and inhibiting fruit decay.

Key words: Chinese Bayberry (Myrica rubra), nitric oxide, metabolism of active oxygen, decay, physiological index

中图分类号:

S641.2

杨虎清;吴峰华;周存山;王允祥. NO对杨梅采后活性氧代谢和腐烂的影响[J]. 林业科学, 2010, 46(12): 70-74.

Yang Huqing;Wu Fenghua;Zhou Cunshan;Wang Yunxiang. Effects of Nitric Oxide on Active Oxygen Metabolism and Fruit Decay in Postharvest Chinese Bayberry[J]. Scientia Silvae Sinicae, 2010, 46(12): 70-74.

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NO对杨梅采后活性氧代谢和腐烂的影响

Effects of Nitric Oxide on Active Oxygen Metabolism and Fruit Decay in Postharvest Chinese Bayberry

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