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林业科学 ›› 1995, Vol. 31 ›› Issue (2): 125-131.

• 论文及研究报告 • 上一篇    下一篇

马尾松针叶中挥发性成分的鉴定及其对马尾松毛虫的触角电位反应*

赵成华,伍德明,阎云花,李群   

  1. 中国科学院动物研究所 北京 100080
  • 收稿日期:1993-11-19 出版日期:1995-03-25 发布日期:1995-03-25

ANALYSIS OF MASSON PINE NEEDLE VOLATILES AND THEIR ELECTROANTENNOGRAM ACTIVITY WITH MASSON PINE CATERPILLAR

Chenghua Zhao,Deming Wu,Yunhua Yan,Qun Li   

  1. Chinese Academy of Sciences Institute of Zoology Beijing 100080
  • Received:1993-11-19 Online:1995-03-25 Published:1995-03-25

摘要:

马尾松Pinus massoniana针叶的挥发物由溶剂提取和真空蒸馏制得。用气相色谱和气相色谱-质谱, 由挥发物中鉴定出了20种成分。它们主要是单萜烯、单萜烯醇、单萜烯乙酸酯和倍半萜烯。用正常和剪除触角的雌蛾研究针叶挥发物在马尾松毛虫Dendrolimus punctutas产卵场所选择中的作用。试验表明正常雌蛾在针叶上的产卵量要比剪除触角的高得多。触角电位(EAG)研究表明无论是交配的还是未交配的雌、雄蛾对针叶挥发物都有强烈的EAG反应。用制备气相色谱对挥发物分馏并测定各馏分对来交配和交配雌蛾的EAG活性。含有某些单萜烯、单萜烯酸及单萜烯乙酸酯的馏分具有较高的EAG活性, 其中主要为β-蒎烯的馏分能引起最大的EAG反应。

关键词: 马尾松, 针叶挥发物, 萜烯, 马尾松毛虫, 嗅觉, 触角电位

Abstract:

Needle volatiles of Masson pine, Pinus massoniana were obtained by solvent extraction and following vacuum distillation.20 Components were identified from the volatiles with GC and GC-MS.They were mainly monoterpenes, monoterpene alcohols, monoterpene acetates and sesquiterpenes. The role of the needle volatiles in the selection of oviposition site for the masson pine caterpillar, Dendrolimus punctatus was investigated with normal and antennectomized female moths.The experiments showed that normal moths deposited much more eggs on the pine needles than antennectomized ones.Electroantennogram(EAG)study showed that both sexes of unmated and mated moths had strong EAG responses to the needle volatiles.The volatiles were fractionated by preparative GC and GC fractions were assayed for EAG activities to unmated and mated females.EAG activity was found in the fractions containing some of monoterpenes, monoterpene alcohols and monoterpene acetates.The largest EAG response was elicited by the fraction mainly containing β-pinene.

Key words: Pinus massoniana, Needle volatiles, Terpenes, Dendrolimus punctatus, Olfaction, Electroantennogram