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林业科学 ›› 2007, Vol. 43 ›› Issue (07): 55-60.doi: 10.11707/j.1001-7488.20070710

• 论文及研究报告 • 上一篇    下一篇

落叶松毛虫对兴安落叶松6种挥发物的触角电位反应

严善春 刘英胜 王琪 程红 张健 迟德富   

  1. 东北林业大学,哈尔滨150040
  • 收稿日期:2006-02-27 修回日期:1900-01-01 出版日期:2007-07-25 发布日期:2007-07-25

Electroantennogram Responses of Dendrolimus superans to Six Volatiles of Larix gmelinii

Yan Shanchun,Liu Yingsheng,Wang Qi,Cheng Hong,Zhang Jian,Chi Defu   

  1. Northeast Forestry University Harbin 150040
  • Received:2006-02-27 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

摘要:

利用触角电位技术(EAG)测定了落叶松毛虫未交尾雌蛾、已交尾雌蛾、未交尾雄蛾对R型α-蒎烯、S型α-蒎烯、罗勒烯、S型β-蒎烯、水芹烯、莰烯等6种兴安落叶松挥发物的触角电位反应。结果表明:不同生理状况的落叶松毛虫对6种挥发物的7个浓度的EAG反应中,除对0.1μL.μL-1水芹烯的EAG反应值是未交尾雌虫>已交尾雌虫>未交尾雄虫外,对水芹烯的其它不同浓度以及其它挥发物的EAG反应值均是已交尾雌蛾>未交尾雌蛾>未交尾雄蛾。未交尾雌、雄蛾的EAG反应差异显著(P<0.05),已交尾雌蛾与未交尾雌、雄蛾之间差异极显著(P<0.01),表明已交尾雌蛾对寄主植物气味更为敏感。落叶松毛虫未交尾雌蛾、已交尾雌蛾、未交尾雄蛾对水芹烯的EAG反应均比较敏感。在暗周期内已交尾雌蛾与未交尾雄蛾的活跃期在20:00—4:00,与其羽化、交尾、产卵期一致。

关键词: 落叶松毛虫, 植物挥发物, 触角电位反应, 兴安落叶松

Abstract:

Dendrolimus superans is one of the important pests feeding the needles of Larix gmelinii. The electroantennogram (EAG) responses of D. superans were tested to the standard compounds: (1R)-(+)-α-pinene, (1S)-(-)-α-pinene, ocimene, (1S)-(-)-β-pinene, R(-)-α-phellandrene and camphene, the main profiles of the volatiles from L. gmelinii. The result showed a regularity that the EAG responses follow the regularity: the virgin females>mated females>males no mated to 0.1 μL·μL-1 R(-)-α-phellandrene, and the mated females>virgin females>males no mated to the other volatiles and concentrations. There were significant differences between the EAG responses of virgin females and males no mated (P<0.05), and the EAG responses of mated females had an extremely significant difference from that of virgin females and males no mated (P<0.01), which showed that the mated females are more active to the volatiles of its host plant. The EAG responses of the mated females, virgin females, males no mated were more sensitive to R(-)-α-phellandrene. In dark period, activity time for the EAG responses of mated females and males no mated is during 20:00—4:00 o'clock,the same to its eclosion, mating and oviposition period.

Key words: Dendrolimus superans, plant volatiles, EAG response, Larix gmelinii