关键词: 高卢蜜环菌, 遗传多样性, IGS-RFLP, RAMS

Abstract:

The IGS of 23 isolates of Armillaria gallica from three continentals of Northern Hemisphere were amplified with primers LR12R and O-1,and digested by AluⅠ,Hae Ⅲ,Hinfl,TagⅠ restriction enzyme,respectively.six RFLP patterns were observed,the patterns of gal(A) and gal(B) were only seen in Chinese isolates,gal(C) was occurred around the northern hemisphere,including USA,Canada,Germany,Poland,Luxembourg and China,gal(D) pattern was seen in a European isolate,gal(E) was only found in American isolates.Based on the parsimony tree of RFLP results,four A.gallica sub-populations were found from these isolates and named the Chinese(CH),European(EU),North American(NA),and Asian(AS) sub-populations.The Random amplified microsatellites (RAMS) experiments were carried out with primer TCG,CGA,M13,and GAAA,respectively.Based upon the parsimony tree of RAMS results,these isolates were separated into four clades,i.e.the European (EU),Chinese (CH),North American-Chinese (NACH),and North American-European (NA-EU) geographical lineage.Two groups emerged in the NA isolates of A.gallica, and they are more close to EU and CH respective than between.The research suggested that the RAMS are quite effective way for analysis of genetic diversity of fungi.

Key words: Armillaria gallica, Genetic diversity, IGS-RFLP, Random amplified microsatellite (RAMS)