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林业科学 ›› 2002, Vol. 38 ›› Issue (5): 169-174.doi: 10.11707/j.1001-7488.20020529

• 研究简报 • 上一篇    下一篇

用RAPD标记进行鹅掌楸杂种识别和亲本选配

李周岐 王章荣   

  1. 西北农林科技大学林学院,杨凌712100;南京林业大学森林资源与环境学院,南京210037
  • 收稿日期:2001-04-15 修回日期:1900-01-01 出版日期:2002-09-25 发布日期:2002-09-25

RAPD MARKERS USED FOR THE HYBRID IDENTIFICATION AND PARENTS CHOICE IN LIRIODENDRON

Li Zhouqi,Wang Zhangrong   

  1. Forestry College, Northwest Sci Tech University of Agriculture and Forestry Yangling712100;College of Forest Resources and Environment, Nanjing Forestry University Nanjing210037
  • Received:2001-04-15 Revised:1900-01-01 Online:2002-09-25 Published:2002-09-25

关键词: 鹅掌楸属, RAPD, 遗传距离, 杂种识别, 亲本选配

Abstract:

According to the genetic difference detected by means of RAPD markers, the methods of distinguish among 2 Liriodendron species and their interspecific hybrid were discussed, and the relationship between genetic distances of parents and their hybrids performance were analysed. Of the total 136 bands amplified with 25 10-mer primers, 3 presented in all 5 L.chinense sample trees, but absented in all 5 L.tulipifera sample trees. On the contrary, 8 presented in all 5 L.tulipifera sample trees, but absented in all 5 L.chinense sample trees. The 11 fragments could be detected in all the 6 hybrids sample trees. It was considered that 11 fragments could be used as markers in the identification. The range of genetic distance among 5 L.chinense sample trees, among the sample trees between L.chinense and L.tulipifera and between L.chinense and the hybrid were not overlapped. Therefore, the genetic distance between an unidentified tree and a L.chinense tree could also be used in the hybrid identification. The 16 sample trees were grouped correctly. So, the UPGMA (unweighted pair group methods of arithmetic (average))cluster analysis method was useful in the (hybrid) species identification. The mean height and basae diameter increment of families after the first growing season showed a significant guadratic cure correlation with genetic distances of parents. Therefore, the genetic distance had high potential for the choice of parents and the heterosis prediction in cross breeding of Liriodendron.

Key words: Liriodendron, RAPD, Genetic distance, Hybrid identification, Parents choice