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林业科学 ›› 2011, Vol. 47 ›› Issue (5): 171-176.doi: 10.11707/j.1001-7488.20110529

• 研究简报 • 上一篇    下一篇

TCS基因转化泡桐及抗病能力

刘静1, 黄艳艳1, 翁曼丽2, 罗磊1, 张虹1, 王长宪1, 牛庆霖3, 冯殿齐1   

  1. 1. 泰山林业科学研究院 泰安 271000;2. 中国科学院遗传与发育生物学研究所 北京 100101;3. 山东农业大学 泰安 271018
  • 收稿日期:2010-06-03 修回日期:2010-11-30 出版日期:2011-05-25 发布日期:2011-05-25
  • 通讯作者: 冯殿齐

Transformation of TCS Gene to Paulownia elongata and Its Virus Resistance

Liu Jing1, Huang Yanyan1, Weng Manli2, Luo Lei1, Zhang Hong1, Wang Changxian1, Niu Qinglin3, Feng Dianqi1   

  1. 1. Taishan Institute of Forestry Science Tai'an 271000;2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101;3. Shandong Agricultural University Tai'an 271018
  • Received:2010-06-03 Revised:2010-11-30 Online:2011-05-25 Published:2011-05-25

关键词: 泡桐, TCS基因, 农杆菌介导, 稳定性, 病毒抗性

Abstract:

In this study, the trichosanthin (TCS) gene, which was cloned from Trichosanthes kirilowii, was transformed into Paulownia elongata through agrobacterium-mediated method. The conversion rate was 29.2% with 86 transformed strains obtained. The target gene had approximately 50% positive expression rate in plantlets through 11 times subculture and PCR detection for 4 times. And the PCR detection of the plants in field showed that the positive rate was between 40% and 50%. No positive expression of the labeled product of witches' broom was found in the transformed plants after infection susceptible experiment and molecular detection, meanwhile, the virus infection rate of the control was up to 15.0%. Thus, it indicated that, compared to the control, the transformed plants showed higher resistance to the witches' broom disease.

Key words: Paulownia elongata, TCS gene, agrobacterium-mediated, stability, virus resistance

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