关键词: 翠菊, 下胚轴, 胚性愈伤组织, 植株再生

Abstract:

This paper reported the successful embryogenic callus induction and somatic embryo plantlet regeneration from hypocotyls of Callistephus chinensis ‘Bouquet Scarlet’. Experimental results indicated that plant hormone types and their concentrations， carbohydrate sources and sucrose concentrations had varied effect on embryogenic callus induction from hypocotyls. The appropriate medium for embryogenic callus induction of C. chinensis ‘Bouquet Scarlet’ was MS+ 0.5 mg·L^-16-BA + 0.5 mg·L^-1 IBA， and with the medium the embryogenic callus induction rate was 27.1％.Sucrose at 30 g·L^-1 was the suitable carbohydrate source and concentration. The suitable medium for plantlet regeneration of C. chinensis ‘Bouquet Scarlet’ embryogenic calli was MS+0.5 mg·L^-1 6-BA + 0.5 mg·L^-1 IBA + 550 mg·L^-1 proline， and with the medium the plantlet regeneration rate was 46.7%.

Key words: Callistephus chinensis &lsquo, Bouquet Scarlet&rsquo, hypocotyl, embryogenic callus, plantlet regeneration