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林业科学 ›› 2009, Vol. 12 ›› Issue (2): 40-47.doi: 10.11707/j.1001-7488.20090207

• 论文 • 上一篇    下一篇

NaCl 胁迫对构树组培苗抗氧化酶活性及同工酶谱的影响

李荣锦1,2 张 敏1,2 蒋泽平2 黄利斌2 王宝松2 季永华2 方炎明1   

  1. (1.南京林业大学森林资源与环境学院 南京 210037;2.江苏省林业科学研究院 南京 211153)
  • 收稿日期:2009-07-10 修回日期:1900-01-01 出版日期:2009-02-25 发布日期:2009-02-25

Effect of NaCl Stress on Antioxidant Enzyme Activities andIsoenzyme Pattern of Broussonetia papyrifera Plantlets

Li Rongjin1,2,Zhang Min1,2,Jiang Zeping2,Huang Libin2,Wang Baosong2,Ji Yonghua2,Fang Yanming1   

  1. (1. College of Forestry Resources and Environment, Nanjing Forestry University Nanjing 210037; 2. Jiangsu Academy of Forestry Nanjing 211153)
  • Received:2009-07-10 Revised:1900-01-01 Online:2009-02-25 Published:2009-02-25

摘要:

摘 要:构树组培苗在50,75,100,125 和 150 mmol•L-1 NaCl胁迫下,SOD,POD,CAT活性和这3种抗氧化酶同工酶谱的变化情况,以及不同浓度NaCl对可溶性蛋白、脯氨酸和MDA含量的影响。结果表明:NaCl浓度小于100 mmol•L-1时可溶蛋白含量升高,随着NaCl浓度的增加可溶蛋白含量降低。低浓度NaCl使SOD活性略有降低,随着浓度升高活性逐渐增强;SOD同工酶谱在NaCl胁迫后变化明显。POD活性变化趋势与SOD相似,而POD同工酶谱略有变化。CAT活性明显提高,而其同工酶谱变化甚微。此外,NaCl胁迫还导致脯氨酸含量明显升高,而MDA含量呈现先升高后降低的趋势。

关键词: 关键词:构树, NaCl胁迫, 抗氧化酶, 同工酶

Abstract:

Abstract: The activities and isoforms of superoxide dismutase peroxidase (POD) and catalase (CAT), as well as the contents of proline, soluble protein (SOD) and malondialdehyde (MDA) in plantlets of Broussonetia papyrifera subjected to salt stress treatments of 50, 75, 100, 125 and 150 mmol•L-1 NaCl were studied. Our results demonstrated that soluble protein content increased with NaCl concentration at low level of NaCl concentration, and it decreased when NaCl concentration exceeds 100 mmol•L-1. SOD activity showed a slight decrease in low concentration of NaCl and then an increase with further increase in NaCl concentration. Moreover, the isoforms of SOD were affected by salt stress. POD exhibited a similar trend in enzyme activity as compared to SOD, However, the POD isoforms were minimally affected by NaCl treatment. CAT activity was increased by salt treatment. Moreover, proline contents markedly increased while the contents of MDA was increased at first and decreased subsequently in response to salt stress.

Key words: Key words: Broussonetia papyrifera, salt stress, antioxidant enzyme, isoenzyme