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林业科学 ›› 2009, Vol. 12 ›› Issue (12): 54-58.doi: 10.11707/j.1001-7488.20091209

• 论文 • 上一篇    下一篇

构树SRAP分子标记*

刘志远1,2 范卫红1,2 沈世华1   

  1. 1.中国科学院植物研究所 光合作用与环境分子生理学重点实验室 北京 100093; 2.中国科学院研究生院 北京 100039
  • 收稿日期:2008-11-12 修回日期:1900-01-01 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 沈世华
  • 基金资助:
     

SRAP Marker in Broussonetia papyrifera

Liu Zhiyuan1,2,Fan Weihong1,2,Shen Shihua1   

  1. 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany,Chinese Academy of Sciences Beijing 100093; 2.Graduate University of Chinese Academy of Sciences Beijing 100039
  • Received:2008-11-12 Revised:1900-01-01 Online:2009-12-25 Published:2009-12-25
  • Supported by:
     

摘要:

建立构树DNA提取方法及最佳SRAP-PCR反应体系,用SRAP分子标记研究构树种质资源的遗传多样性。结果表明:SRAP扩增的清晰条带大小在100~1 000 bp,用17对引物对21份材料扩增出439条带,其中多态性条带319条,占72.6%,Shannon信息指数(I)均值为0.227 5,物种水平的Nei基因多样性(H)均值为0.133 6,表明构树的遗传多样性不高。聚类分析结果显示与地理分布有相关性。同时发现一些特异性条带,可以转化为SCAR标记,这为构树的遗传图谱构建、遗传育种等研究提供了参考。

关键词: 构树, SRAP分子标记, 遗传多样性, SCAR标记

Abstract:

Broussonetia papyrifera is important with its environmental protection value and economic value. In this report,genetic diversity and genetic relationship were analyzed by SRAP marker. A DNA extraction protocol was developed for B. papyrifera and then an optimal SRAP-PCR system was established. The results showed that the bands ranged from 100 to 1 000 bp were clearly displayed. A total 439 bands were obtained by 17 pairs of primers, among which 319 bands were polymorphic, accounting for more than 72.6% of the total bands. The average Neis genetic diversity and Shannons information diversity index were respectively 0.227 5 and 0.133 6,which indicated genetic diversity of B. papyrifera was not high. The cluster analysis revealed there was relationship between the genetic variation and geographical distribution of B. papyrifera. Some specific bands were founded and could be converted into SCAR markers. This research would provide reference for making genetic map and breeding in B. papyrifera.

Key words: Broussonetia papyrifera, SRAP marker, genetic diversity, SCAR marker

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