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林业科学 ›› 2000, Vol. 36 ›› Issue (2): 93-97.doi: 10.11707/j.1001-7488.20000215

• 论文及研究报告 • 上一篇    下一篇

转Bt基因和蛋白酶抑制剂基因杨树抗虫性的研究

李明亮 张辉 胡建军 韩一凡 田颖川   

  1. 中国林业科学研究院林业研究所,北京100091;中国科学院微生物研究所,北京100080
  • 收稿日期:1999-05-04 修回日期:1900-01-01 出版日期:2000-03-25 发布日期:2000-03-25

STUDY ON INSECT-RESISTANT TRANSGENIC POPLAR PLANTS CONTAINING BOTH BT AND PI GENE

Li Mingliang,Zhang Hui,Hu Jianjun,Han Yifan,Tian Yingchuan   

  1. The Research Institute of Foresty, CAF Beijing 100091;Institute of Microbiology, Chinese Academy of Sciences Beijing 100080
  • Received:1999-05-04 Revised:1900-01-01 Online:2000-03-25 Published:2000-03-25

摘要:

虫害是杨树造林生产中所面临的一大难题。通过农杆菌介导,将经过人工改造的苏云金杆菌杀虫结晶蛋白(Bt)基因转化杨树,得到杨树再生植株,然后,再将蛋白酶抑制剂基因(PI)导入已含Bt基因的转基因杨树,经PCR检测和Southern杂交分析证明,最终获得既含有Bt基因又含有蛋白酶抑制剂基因的转基因杨树植株。利用这种杨树叶片饲喂舞毒蛾幼虫的杀虫试验结果表明,转基因杨树具有明显的杀虫活性,同时表明含有Bt基因和蛋白酶抑制剂基因双基因的植株其抗虫能力明显高于仅含单一Bt基因的植株。为探索培育高抗虫性杨树开辟了一条新途径。

关键词: 欧洲黑杨, Bt基因, 蛋白酶抑制剂基因, 抗虫性

Abstract:

Poplars was seriously attacked by insects usually in many areas and insect resistant poplars are expected in forest production. In the experiment, the modified Bacillus thuringiensis (Bt) δ-endotoxin gene and proteinase inhibitor(PI)gene were contransformed to the explants of Populus nigra mediated by Agrobacterium tumefaciens. The integration of Bacillus thuringiensis gene and proteinase inhibitor(PI)gene were confirmed by PCR analysis and Southern hybridization. The results of bioassay showed that the transgenic plants had toxicity to the larvae of Lymantria dispar L., and that the transgenic plants containing Bt gene and PI gene had enhanced toxicity to larvae by comparison with plants containing only Bt gene.

Key words: Populus nigra, Bt gene, Proteinase inhibitor gene, Insect-resistance