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›› 1990, Vol. 26 ›› Issue (6): 506-514.

• 论文及研究报告 • 上一篇    下一篇

杉木半穿刺线虫病的研究

蔡秋锦 龚其锦 林邦超   

  1. 福建林学院;沙县林业委员会
  • 收稿日期:1989-07-17 修回日期:1900-01-01 出版日期:1990-11-25 发布日期:1990-11-25

STUDIES ON TYLENCHULUS SEMIPENETRANS COBB ATTACKING CUNNINGHAMIA LANCEOLATA

Cai Qioujing,Gong Qijing,Lin Bangchao   

  1. Fujian Forestry College;Forestry Committee of Shaxian County
  • Received:1989-07-17 Revised:1900-01-01 Online:1990-11-25 Published:1990-11-25

摘要: 近几年,我们对杉木普遍生长衰退及杉苗死亡进行研究,新发现了杉木半穿刺线虫病,病原线虫鉴定为Tylenchulus semipenetrans Cobb。通过定点定时定量系统观测,该线虫在福建年发生10代左右,种群数量自然消长与气温星正相关,与杉木根萌发相一致,单卵培养计测,雌雄比为56:1,线虫最高发育温度为35℃,卵的致死温度为40℃。林地对比定时定量调查表明,虫口密度南坡最大,并随林龄增加而增大。在土壤中垂直分布可达1.2m,而30—40cm土层中虫口数最大,杉木与阔叶树混交较纯杉林虫口数下降26—72%。通过生理生化试验测出,病、健根的呼吸强度、N、P、K和蛋白质含量、酶的活性均差异明显。从病根分离和纯化真菌区系,经对比接种,发现线虫寄生利于镰刀菌入侵。病菌经40—50℃温水浸根10—15min可直接杀死线虫。分别将每亩3kg呋喃丹,6kg甲胺磷,2kg涕灭威用于区组、盆栽和大面积防治,效果为70—80%,发现新寄主还有柳杉和野柿子。

关键词: 杉木, 半穿刺线虫, 生活史, 生理生化, 防治

Abstract: A study on the decline of China fir was made during 1985 —1988. This is a popular disease now occurred in Fujian province of China. The enlarged roots of the trees became as cock-foot caused by the damage of nematode Tylenchulus semipenetrans Cobb,a new host just discovered in addition to citrus. The nematode has 10—12 generations per year. Their generation is metagenesis. Individual development is uncoreplete. Its sex ratio is 56:1 between female and male. Rate of egg hatch in root soaking water is 90%. Its maximum temperatuee for growch is 35℃. Death point of eggs is 40℃. Density of nematodes are increased with the ages of trees and are highest in south slope and lowerest in a mixed stand with Choerospondias axillaris. Distribution of nematodes in soil is coincided with the nutrition roots. Their amounts are mostly in 30—40cm deepth in the middle and lower slope. The aspiration strength and the amounts of N, P, K contained in diseased roots are larger than thoes in healthy ones as well as the activities of multiphenol oxidase, hydrogen peroxidase and iso-peroxidase. Invasion of nematodes induced the parasite of Fusaria in soil; thus caused the decay of roots. Hot water treatment in 40—50℃ soaking the diseased roots had prominently effect to kill the nematodes. Using methamidophos,Carbofuran and ALdicarb in an area of about 24000 acres an effective results of control is 70 —80%. Mixed stands with Paulownia spp.,Choerospondias axillaris, or Sassafras tzumu had less population of nematodes. Cryptomeria fortunei and wild persimmon are also descovered as new hosts.

Key words: China fir, Tylenchulus semipenetrans, Life-history, Biochemistry and physiology, Control