林业科学 ›› 2021, Vol. 57 ›› Issue (4): 173-181.doi: 10.11707/j.1001-7488.20210418
王晓1,韦小丽1,2,*,吴高殷1,陈胜群1
收稿日期:
2020-02-10
出版日期:
2021-04-01
发布日期:
2021-05-21
通讯作者:
韦小丽
基金资助:
Xiao Wang1,Xiaoli Wei1,2,*,Gaoyin Wu1,Shengqun Chen1
Received:
2020-02-10
Online:
2021-04-01
Published:
2021-05-21
Contact:
Xiaoli Wei
摘要:
目的: 研究不同CO2浓度、不同氮素形态及供应量条件下闽楠幼苗光合特性及生长的表现,探索氮肥施用调控闽楠幼苗光合下调现象的方式。方法: 以1年生闽楠实生苗为试验材料,采用开顶式气室进行CO2浓度处理[自然浓度(350±70)μmol·mol-1,升高浓度(700±10)μmol·mol-1],施用不同量硝态氮及铵态氮(中等用量每株0.8 g,增加用量每株1.2 g)培育闽楠幼苗。结果: 与自然CO2浓度处理相比,长时间高浓度CO2处理(88天)后施用中等量硝态氮闽楠幼苗净光合速率降低26.93%(P < 0.05),其核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)以及Rubisco活化酶(RCA)活性、可溶性糖、淀粉、可溶性蛋白含量、苗高、地径净生长量均显著低于自然CO2浓度处理(P < 0.05),说明闽楠幼苗出现光合下调现象并最终影响生长是因为光合关键酶活性降低而非光合产物积累抑制;长时间CO2浓度升高条件下增施硝态氮或施用铵态氮闽楠幼苗净光合速率无显著变化(P>0.05),其Rubisco以及RCA活性、可溶性糖、淀粉、可溶性蛋白含量以及苗高、地径净生长量均提高或无显著变化,表明增施硝态氮或施用铵态氮可以缓解长期高浓度CO2条件对闽楠幼苗光合及生长产生的不良表现。结论: 长期CO2浓度升高条件下,增施硝态氮或铵态氮可提高闽楠幼苗光合关键酶活性,增加有机物含量,最终促进闽楠幼苗生长以缓解其对闽楠幼苗生长的负面影响。
中图分类号:
王晓,韦小丽,吴高殷,陈胜群. CO2浓度升高条件下不同氮素供应对闽楠幼苗光合特性及生长的影响[J]. 林业科学, 2021, 57(4): 173-181.
Xiao Wang,Xiaoli Wei,Gaoyin Wu,Shengqun Chen. Effects of Different Nitrogen Forms and Supply on Photosynthetic Characteristics and Growth of Phoebe bournei Seedlings under Elevated CO2 Concentration[J]. Scientia Silvae Sinicae, 2021, 57(4): 173-181.
表1
不同处理对闽楠幼苗气体交换参数的影响①"
处理 Treatments | 净光合速率 Pn/(μmol·m-2s-1) | 气孔导度 Gs/(mol·m-2s-1) | 胞间CO2浓度 Ci/(μmol·m-2s-1) | 蒸腾速率 Tr/(mmol·m-2s-1) | 水分利用效率 WUE/(μmol·mmol-1) | |
aCO2 | NO3- | 4.53±0.78cd | 0.088±0.02d | 192.8±10.7cd | 2.26±0.48d | 2.04±0.18a |
iNO3- | 5.38±0.51a | 0.121±0.01a | 205.7±2.8b | 2.97±0.22a | 1.81±0.05bcd | |
NH4+ | 4.02±0.69e | 0.086±0.03d | 197.6±11.8c | 2.37±0.54cd | 1.72±0.16d | |
iNH4+ | 4.80±0.25bc | 0.094±0.01cd | 191.8±2.5d | 2.68±0.12b | 1.79±0.05cd | |
eCO2 | NO3- | 3.31±0.83f | 0.070±0.03e | 205.8±11.4b | 1.75±0.56e | 1.93±0.18ab |
iNO3- | 5.11±0.53ab | 0.115±0.01ab | 207.7±1.8ab | 2.69±0.29b | 1.90±0.02bc | |
NH4+ | 4.35±0.36de | 0.105±0.01bc | 212.1±5.1a | 2.59±0.22bc | 1.69±0.10d | |
iNH4+ | 5.01±0.47ab | 0.116±0.01ab | 208.4±6.1ab | 2.82±0.24ab | 1.78±0.15cd |
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