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林业科学 ›› 2021, Vol. 57 ›› Issue (4): 173-181.doi: 10.11707/j.1001-7488.20210418

• 研究简报 • 上一篇    下一篇

CO2浓度升高条件下不同氮素供应对闽楠幼苗光合特性及生长的影响

王晓1,韦小丽1,2,*,吴高殷1,陈胜群1   

  1. 1. 贵州大学林学院 贵阳 550025
    2. 贵州省森林资源与环境研究中心 贵阳 550025
  • 收稿日期:2020-02-10 出版日期:2021-04-01 发布日期:2021-05-21
  • 通讯作者: 韦小丽
  • 基金资助:
    贵州省科技计划项目(黔科合基础[2019]1407号);贵州省高层次创新型人才培养计划(黔科合平台人才[2016]5661);贵州大学林学、生态学一流学科建设项目

Effects of Different Nitrogen Forms and Supply on Photosynthetic Characteristics and Growth of Phoebe bournei Seedlings under Elevated CO2 Concentration

Xiao Wang1,Xiaoli Wei1,2,*,Gaoyin Wu1,Shengqun Chen1   

  1. 1. College of Forestry, Guizhou University Guiyang 550025
    2. Institute for Forest Resources and Environment of Guizhou Guiyang 550025
  • Received:2020-02-10 Online:2021-04-01 Published:2021-05-21
  • Contact: Xiaoli Wei

摘要:

目的: 研究不同CO2浓度、不同氮素形态及供应量条件下闽楠幼苗光合特性及生长的表现,探索氮肥施用调控闽楠幼苗光合下调现象的方式。方法: 以1年生闽楠实生苗为试验材料,采用开顶式气室进行CO2浓度处理[自然浓度(350±70)μmol·mol-1,升高浓度(700±10)μmol·mol-1],施用不同量硝态氮及铵态氮(中等用量每株0.8 g,增加用量每株1.2 g)培育闽楠幼苗。结果: 与自然CO2浓度处理相比,长时间高浓度CO2处理(88天)后施用中等量硝态氮闽楠幼苗净光合速率降低26.93%(P < 0.05),其核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)以及Rubisco活化酶(RCA)活性、可溶性糖、淀粉、可溶性蛋白含量、苗高、地径净生长量均显著低于自然CO2浓度处理(P < 0.05),说明闽楠幼苗出现光合下调现象并最终影响生长是因为光合关键酶活性降低而非光合产物积累抑制;长时间CO2浓度升高条件下增施硝态氮或施用铵态氮闽楠幼苗净光合速率无显著变化(P>0.05),其Rubisco以及RCA活性、可溶性糖、淀粉、可溶性蛋白含量以及苗高、地径净生长量均提高或无显著变化,表明增施硝态氮或施用铵态氮可以缓解长期高浓度CO2条件对闽楠幼苗光合及生长产生的不良表现。结论: 长期CO2浓度升高条件下,增施硝态氮或铵态氮可提高闽楠幼苗光合关键酶活性,增加有机物含量,最终促进闽楠幼苗生长以缓解其对闽楠幼苗生长的负面影响。

关键词: 闽楠, 氮素, 二氧化碳, 光合作用, 光合关键酶

Abstract:

Objective: In this paper, the photosynthetic characteristics and growth performance of Phoebe bournei seedlings under different CO2 concentrations, different nitrogen forms and supply were studied to explore whether nitrogen fertilization could be used to regulate the photosynthesis of P. bournei seedlings. Method: One-year-old P. bournei seedlings were used as experimental materials. The open-top gas chamber was used for CO2 concentration treatment [ambient concentration: (350±70) μmol·mol-1; elevated concentration: (700±10) μmol·mol-1], and different amounts (medium dosage 0.8 g·plant-1, increased dosage 1.2 g·plant-1) of nitrate and ammonium were used to cultivate P. bournei seedlings. Result: Compared with ambient CO2 concentration treatment, after a long period of elevated concentration CO2 treatment (88 days), the net photosynthetic rate of P. bournei seedlings reduced by 26.93% (P < 0.05), and its Rubisco and RCA activity, soluble sugar, and starch, soluble protein content, seedling height and ground diameter were all significantly lower (P < 0.05) than those treated with ambient CO2. The photosynthetic down-regulation phenomenon of P. bournei seedlings eventually affected growth. Under long-term elevated CO2 concentration, the net photosynthetic rate of P. bournei seedlings subjected to increased nitrate or ammonium had no significant changes (P>0.05), its Rubisco and RCA activity, soluble sugar, starch, soluble protein content, and seedlings height and ground diameter increased or there was no significant change, indicating that increasing nitrate or ammonium nitrogen application can alleviate the adverse effects of long-term elevated CO2 concentration conditions on photosynthesis and growth of P. bournei seedlings. Conclusion: Under the condition of long-term elevated CO2 concentration, the application of increased nitrate or ammonium nitrogen can increase the activities of photosynthetic key enzymes, increase the content of organic matter, and eventually promote the growth of P. bournei seedlings to alleviate the negative impact of elevated CO2 concentration on the growth of P. bournei seedlings.

Key words: Phoebe bournei, nitrogen, carbon dioxide, photosynthesis, photosynthetic enzyme

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