CO2浓度升高对舞毒蛾生长发育和体内解毒酶及保护酶活性的影响

doi:10.11707/j.1001-7488.20181211

摘要/Abstract

摘要： [目的]以林业食叶害虫舞毒蛾为对象，研究CO₂浓度升高对个体生长发育、营养利用和体内解毒酶及保护酶的影响。[方法]利用密闭式CO₂人工气候箱研究了不同CO₂浓度（397、550和750 μL·L^-1）对人工饲料饲养舞毒蛾生长发育指标和营养利用的影响，并采用分光光度计法测定CO₂升高对体内保护酶和解毒酶活性的影响。[结果]CO₂浓度升高可延长舞毒蛾卵孵化历期、增加产卵量和孵化率；同时显著延长了幼虫总发育历期，而对蛹和成虫历期影响不显著。CO₂浓度升高可降低舞毒蛾幼虫相对取食率和近似消化率，同时增加食物利用率和食物转化率。750 μL·L^-1 CO₂浓度可显著诱导舞毒蛾幼虫体内超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性，分别比397 μL·L^-1 CO₂浓度处理组增加了32.05%和13.47%；随着CO₂浓度的升高，550 μL·L^-1和750 μL·L^-1 CO₂浓度下细胞色素P450的活性比397 μL·L^-1CO₂浓度下分别增加了78.79%和93.50%；谷胱甘肽S转移酶（GST）活性在550 μL·L^-1 CO₂浓度处理下最高，分别比397 μL·L^-1和750 μL·L^-1CO₂处理组活性增加71.03%和50.00%（P<0.05）。[结论]CO₂浓度升高能够影响舞毒蛾幼虫生长发育和生理代谢功能，同时舞毒蛾通过改变发育历期、增加食物的利用和转化以及改变体内解毒酶和（或）保护酶活性来维持生长以抵抗高浓度CO₂环境。

关键词: 舞毒蛾, 二氧化碳浓度, 生长发育, 营养利用, 气候变化, 森林昆虫

Abstract: [Objective] The present paper aimed to study effects of elevated CO₂ concentration on the growth and development, nutrient utilization and in vivo detoxifying enzymes and protective enzymes of the serious forest defoliator, Lymantria dispar (Lepidoptera: Lymantridae). [Method] The different CO₂ concentrations (397, 550 and 750 μL·L^-1) were applied in the closed CO₂ artificial climate chamber, and the effects on growth and development of L. dispar were measured, and the effects on activities of detoxification enzymes and protective enzymes of L. dispar were determined by spectrophotometry. [Result] The elevated CO₂ concentration was able to prolong the egg hatching period of L. dispar, increased fecundity and hatching rate. At the same time, the elevated CO₂ concentration significantly prolonged the total larval developmental period, however, it had no significant effects on pupae and adult duration. Increased CO₂ concentration decreased the relative feeding rate and the approximate digestibility, but increased the food utilization rate and the conversion rate of digested food by L. dispar. The activities of superoxide dismutase (SOD) and catalase (CAT) in L. dispar larval body were significantly induced by 750 μL·L^-1 of CO₂ concentration, and increased by 32.05% and 13.47% compared with that at 397 μL·L^-1 CO₂ concentration, respectively; With the increase of CO₂ concentration, the activities of cytochrome P450 increased by 78.79% and 93.50% under 550 μL·L^-1 and 750 μL·L^-1 CO₂, respectively, compared with that at 397 μL·L^-1 CO₂. The GST activities were the highest under 550 μL·L^-1 CO₂ concentration, which was 71.03% and 50.00% higher than those in 397 μL·L^-1 and 750 μL·L^-1 treatment groups, respectively. [Conclusion] The elevated CO₂ concentration can affect the growth and physiological metabolism of the L. dispar larvae. At the same time, L. dispar can resist the high concentration of CO₂ through changing the developmental duration, increasing the food utilization and transformation, and changing the activities of detoxifying enzymes or/and protective enzymes to achieve the purpose of maintaining life.

Key words: Lymantria dispar, carbon dioxide concentration, growth and development, nutritional utilization, climate change, forest inscet

中图分类号:

S718.7

苑冉, 李欣悦, 吴韶平, 曹传旺. CO₂浓度升高对舞毒蛾生长发育和体内解毒酶及保护酶活性的影响[J]. 林业科学, 2018, 54(12): 102-109.

Yuan Ran, Li Xinyue, Wu Shaoping, Cao Chuanwang. Effects of Elevated CO₂ Concentration on Growth and Development of Lymantria dispar (Lepidoptera: Lymantridae), as well as on Activities of Detoxifying Enzymes and Protective Enzymes in the Body[J]. Scientia Silvae Sinicae, 2018, 54(12): 102-109.

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CO₂浓度升高对舞毒蛾生长发育和体内解毒酶及保护酶活性的影响

Effects of Elevated CO₂ Concentration on Growth and Development of Lymantria dispar (Lepidoptera: Lymantridae), as well as on Activities of Detoxifying Enzymes and Protective Enzymes in the Body

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