3株产气内生真菌的水果防腐活性及其气体成分分析

doi:10.11707/j.1001-7488.20130612

林业科学 ›› 2013, Vol. 49 ›› Issue (6): 83-89.doi: 10.11707/j.1001-7488.20130612

3株产气内生真菌的水果防腐活性及其气体成分分析

袁志林¹, 陈益存¹, 毛黎娟², 章初龙³, 陈连庆¹

1. 中国林业科学研究院亚热带林业研究所富阳 311400;
2. 浙江大学农生环分析测试中心杭州 310058;
3. 浙江大学生物技术研究所水稻生物学国家重点实验室杭州 310058

收稿日期:2012-04-17 修回日期:2013-04-18 出版日期:2013-06-25 发布日期:2013-07-16
通讯作者: 章初龙
基金资助:
浙江省科技厅公益技术研究农业项目(2011C22045); 中央级公益性科研院所基本科研业务专项资金(RISF6902); 国家高技术研究发展计划(863计划)课题(2011AA10A205-03)。

Activity of Three Volatile-Producing Endophytic Muscodor Strains in Controlling Fruit Postharvest Disease and Analysis of the Volatile Components

Yuan Zhilin¹, Chen Yicun¹, Mao Lijuan², Zhang Chulong³, Chen Lianqing¹

1. Institute of Subtropical Forestry, Chinese Academy of Forestry Fuyang 311400;
2. Analysis Center of Agrobiology and Environmental Science, Zhejiang University Hangzhou 310058;
3. State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou 310058

Received:2012-04-17 Revised:2013-04-18 Online:2013-06-25 Published:2013-07-16

摘要/Abstract

摘要：

从极端濒危植物百山祖冷杉针叶组织中分离到3株属于炭角菌科产气霉属的内生菌,这些菌株能产生强烈的挥发性物质,对多种植物病原菌具有明显的抑制或致死功能; 水果保鲜试验表明固体发酵菌剂能有效防治草莓灰葡萄孢霉、苹果轮纹病菌和油桃褐腐病菌等引起的水果采后病害。固相微萃取和气质联用(SPME-GC-MS)分析表明,3个菌株产生的气体成分及含量存在较大差异,M25成分单一,而M112-2和M153成分较复杂,这种差异性与其生物学活性有一定的相关性。主要气体物质为二甲酯丙酸、甲基-2-甲基丙酸酯和β-水芹烯等。

关键词: 产气霉, 固相微萃取, 抑菌活性

Abstract:

Three endophytic Muscodor isolates isolated from needle tissues of the critically threatened Abies beshanzuensis can produce intense volatiles in vitro, which in general cause obvious inhibition or even death to a wide range of plant pathogens, indicating their strong antibiotic activity. In this paper, the fungal agents derived from solid-state fermentation were applied to efficiently control the fruit postharvest diseases, including Botrytis cinerea, Physalospora pyricola and Monilinia fructicola. The SPME-GC-MS technique was used to detect the volatile profiling of Mucodor, and the result showed that there were obvious difference in the chemical components and relative content among the three Mucodor isolates. Muscodor sp. M25 had relatively simple chemical composition of volatiles, while Muscodor fengyangensis M112-2 and M153 had complicated composition. The difference in their chemical composition of volatiles was, to some degree, correlated with their bioactivity. Propanoic acid, 2-methyl-, propanoic acid, 2-methyl-, methyl ester and β-phellandrene were the main volatile small molecules. Thus, those Muscodor isolates would be ideal candidates to be exploited as novel ‘myco-fumigation’ agents.

Key words: Muscodor, solid phase micro-extraction, anti-pathogen activity

中图分类号:

S763.15

袁志林, 陈益存, 毛黎娟, 章初龙, 陈连庆. 3株产气内生真菌的水果防腐活性及其气体成分分析[J]. 林业科学, 2013, 49(6): 83-89.

Yuan Zhilin, Chen Yicun, Mao Lijuan, Zhang Chulong, Chen Lianqing. Activity of Three Volatile-Producing Endophytic Muscodor Strains in Controlling Fruit Postharvest Disease and Analysis of the Volatile Components[J]. Scientia Silvae Sinicae, 2013, 49(6): 83-89.

参考文献

聂继云, 李静,李海飞,等. 2007. 我国水果防腐保鲜剂的使用及其检测方法. 植物保护, 33(6): 26-30.

袁兵兵,杨姗姗,陈静. 2011. 微生物源槐糖脂对水果致腐真菌的抑制作用. 应用与环境生物学报, 17 (3): 330-333.

Banerjee D, Strobel G A, Booth E, et al. 2010. An endophytic Myrothecium inundatum producing volatile organic compounds. Mycosphere, 1(3): 229-240.

Ezra D, Hess W M, Strobel G A. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology, 150(12): 4023-4031.

Kudalkar P, Strobel G,Riyaz-Ul-Hassan S, et al. 2012. Muscodor sutura, a novel endophytic fungus with volatile antibiotic activities. Mycoscience, DOI: 10.1007/s10267-011-0165-9.

Mercier J, Lego S F, Smilanick J L.2010. In-package use of Muscodor albus volatile-generating sachets and modified-atmosphere liners for decay control in organic table grapes under commercial conditions. Fruits, 65(1): 31-38.

Redman R S, Sheehan K B, Stout R G, et al. 2002. Thermotolerance generated by plant/fungal symbiosis. Science, 298(5598): 1581.

Stierle A, Strobel G, Stierle D. 1993. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science, 260 (5105): 214-216.

Strobel G A, Spang S, Kluck K,et al. 2008. Synergism among volatile organic compounds resulting in increased antibiosis in Oidium sp. FEMS Microbiology Letters, 283(2): 140-145.

Strobel G A, Dirksie E, Sears J,et al. 2001. Volatile antimicrobials from Muscodor albus, a novel endophytic fungus. Microbiology, 147(11): 2943-2950

Tomsheck A R, Strobel G A, Booth E,et al. 2010. Hypoxylon sp., an endophyte of Persea indica, producing 1, 8-cineole and other bioactive volatiles with fuel potential. Microbial Ecology, 60(4): 903-914.

Varma A, Savita Verma, Sudha,et al. 1999. Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Applied and Environmental Microbiology, 65(6): 2741-2744.

Worapong J, Strobel G A, Ford E,et al. 2001. Muscodor albus anam. gen. et sp. nov., an endophyte from Cinnamomum zeylanicum. Mycotaxon, 79: 67-79.

Xu X B, Qin G Z, Tian S P. 2008. Effect of microbial biocontrol agents on alleviating oxidative damage of peach fruit subjected to fungal pathogen. International Journal of Food Microbiology, 126: 153-158.

Yuan Z L, Rao L B, Chen Y C, et al. 2011. From pattern to process: species and functional diversity in fungal endophytes of conifer Abies beshanzuensis. Fungal Biology, 115(3): 197-213.

Yuan Z L, Chen Y C, Xu B G,et al. 2012. Current perspectives on the volatile-producing fungal endophytes. Critical Reviews in Biotechnology, 32(4): 363-373.

Zhang C L, Wang G P, Mao L J,et al. 2010. Muscodor fengyangensis sp. nov. from southeast China: morphology, physiology and production of volatile compounds. Fungal Biology, 114(10): 797-808.

Zhang H Y, Zheng X D, Fu C X,et al. 2005. Postharvest biological control of gray mold rot of pear with Cryptococcus laurentii. Postharvest Biology and Technology, 35(1): 79-86.

Zong Y Y, Liu J, Li B Q,et al. 2010. Effects of yeast antagonists in combination with hot water treatment on postharvest diseases of tomato fruit. Biological Control, 54(3): 316-321.

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3株产气内生真菌的水果防腐活性及其气体成分分析

Activity of Three Volatile-Producing Endophytic Muscodor Strains in Controlling Fruit Postharvest Disease and Analysis of the Volatile Components

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