台湾杉遗传多样性的ISSR分析

doi:10.11707/j.1001-7488.20140608

摘要/Abstract

摘要：

采用ISSR分子标记技术，对台湾杉4个自然居群的遗传多样性水平和遗传结构进行研究。从100条引物中筛选出10条，得到78个清晰的扩增条带，其中多态性条带61个。POPGENE分析结果表明：台湾杉在物种水平（多态条带百分率PPB=78.21%，Nei’s基因多样性指数H=0.249 4，Shannon’s信息指数I=0.377 8）和居群水平（PPB=52.56%，H=0.178 3，I=0.267 7）均具有中等水平的遗传多样性。AMOVA分析表明，4个居群间出现一定程度的遗传分化，且遗传变异主要发生在居群内。UPGMA聚类和Mantel检测结果表明，居群间的地理距离与遗传距离之间不存在显著相关性（r=0.043 3， P=0.435 6）。根据台湾杉的遗传变异规律，提出有效的保护措施。

关键词: 台湾杉, ISSR, 遗传多样性, 遗传结构, 保护策略

Abstract:

The genetic diversity and genetic structure of four natural populations of Taiwania cryptomerioides were investigated using inter-simple sequence repeat (ISSR). Of the 100 primers screened, 10 primers were selected and they generated 78 stable and distinct bands, among which 61 bands were polymorphic. The result of POPGENE indicated that T. cryptomerioides had a medium level of genetic variation at the species (the percentage of polymorphic bands PPB=78.21%, Nei's gene diversity H=0.249 4, Shannon's information index I=0.377 8) and population levels (PPB=52.56%, H=0.178 3, I=0.267 7). There was to a certain level genetic differentiation among four populations (G_ST: 0.285 1; Shannon's genetic differentiation: 29.14%; AMOVA genetic differentiation: 28.31%), and most of the genetic differentiation occurred within populations. The UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical and genetic distance(r=0.043 3, P=0.435 6). Based on these findings, we propose appropriate conservation strategies for this endangered species.

Key words: Taiwania cryptomerioides, ISSR, genetic diversity, genetic structure, conservation strategy

中图分类号:

李江伟, 杨琴军, 刘秀群, 季华, 张卓文. 台湾杉遗传多样性的ISSR分析[J]. 林业科学, 2014, 50(6): 61-66.

Li Jiangwei, Yang Qinjun, Liu Xiuqun, Ji Hua, Zhang Zhuowen. Genetic Diversity of Taiwania cryptomerioides Detected by ISSR Analysis[J]. Scientia Silvae Sinicae, 2014, 50(6): 61-66.

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