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林业科学 ›› 2014, Vol. 50 ›› Issue (1): 63-68.doi: 10.11707/j.1001-7488.20140110

• 论文与研究报告 • 上一篇    下一篇

HSP ∷ FT转基因杨树热激开花的影响因素

贾小明, 张焕玲, 唐明   

  1. 西北农林科技大学林学院 西部环境与生态教育部重点实验室 杨凌 712100
  • 收稿日期:2013-06-07 修回日期:2013-10-22 出版日期:2014-01-25 发布日期:2014-01-25
  • 基金资助:

    国家自然科学基金项目(31300563);陕西省自然科学基金项目(2012JM3006);国家林业局“948”项目(2013-4-38)。

Factors Influencing the Heat Shock Flowering of HSP ∷ FT Transgenic Poplar

Jia Xiaoming, Zhang Huanling, Tang Ming   

  1. Key Laboratory of Environment and Ecology in Western China of Ministry of Education College of Forestry, Northwest A & F University Yangling 712100
  • Received:2013-06-07 Revised:2013-10-22 Online:2014-01-25 Published:2014-01-25
  • Contact: 唐明

摘要:

以转 HSP ∷ FT 1基因的白杨派杂种无性系(欧洲山杨×美洲山杨)为试材,系统研究影响FT基因热激表达诱导杨树早期开花的各种因素,为 HSP ∷ FT基因在木本植物中有效诱导正常开花提供基础数据。结果表明:不同转基因株系、同一株系的不同单株、转基因材料的株高及年龄(热激前温室生长时间)、热激温度及热激时间、环境温度等因素均影响转基因植株的热激开花。同一基因型的不同转基因株系以及同一株系不同单株热激诱导开花存在显著差异。开花率随热激材料株高的增加呈上升趋势,株高小于30 cm的转基因植株不能诱导开花。转基因植株在温室培养的时间越长,热激后开花越早,开花率越高。热激温度影响热激材料的初始开花时间、开花率及正常花序得率,最佳热激温度为40℃。转基因植株的热激诱导开花,在早期主要受每天热激时间的影响,在后期主要受持续热激天数的影响,持续热激3周以上可有效提高开花率及正常花序得率。热激前转基因材料在较低的环境温度下生长,有利于热激诱导开花及获得正常花序。

关键词: FT基因, 杨树, 热激诱导, 早期开花, 影响因素

Abstract:

In order to understand the underline mechanism for inducing normal flowering efficiently by HSP ∷ FT construct on forest trees, transgenic poplar plants (Populus tremula × P. tremuloides) containing HSP ∷ FT 1 were used as the experimental material to investigate factors affecting FT gene expression to induce precociously flowering of poplar by heat shock treatment in this paper. The results showed that the factors, such as transgenic lines and ramets, plant size and age, heat shock temperature, heat induction duration, and environment temperature, all affected FT gene expression to induce flowering of transgenic plants. Different transgenic lines and different ramets in the same line varied significantly in the induced flowering. Plants with a height lower than 30 cm failed to flower. Flowering frequency increased with increasing height of plants. Heat shock temperature affected initial flowering time, flowering frequency and normal catkin yields. The optimum temperature was 40℃. Flowering of transgenic plants by heat shock was mainly influenced by daily heat induction hours at early stages and by heat induction duration at later stages. The continue heat treatment for more than 3 weeks was efficient to induce flowering. It was beneficial to flowering induction and normal catkin development if transgenic plants grew at lower environment temperature before heat treatment.

Key words: FT gene, poplar, heat shock induction, precociously flowering, influence factors

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