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林业科学 ›› 2010, Vol. 46 ›› Issue (10): 50-56.doi: 10.11707/j.1001-7488.20101008

• 论文 • 上一篇    下一篇

七里峪不同海拔茶条槭种群的遗传多样性

闫女,王丹,高亚卉,郝晓杰,王祎玲   

  1. 山西师范大学生命科学学院 临汾 041004
  • 收稿日期:2009-08-31 修回日期:2010-02-06 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 王祎玲

Genetic Diversity of Acer ginnala Populations at Different Elevation in Qiliyu Based on ISSR Markers

Yan Nü;Wang Dan;Gao Yahu;iHao Xiaojie;Wang Yiling   

  1. <i>College of Life Sciences;Shanxi Normal University Linfen</i> 041004
  • Received:2009-08-31 Revised:2010-02-06 Online:2010-10-25 Published:2010-10-25

摘要:

利用ISSR分子标记研究七里峪1 200~2 000 m 8个海拔梯度茶条槭种群的遗传多样性和遗传结构。11条引物共得到78个重复性好的位点,在物种水平上,多态位点78个,多态百分率为100%。在8个茶条槭种群中,总遗传多样性指数<i>I</i>(Shannon多样性指数)、<i>H</i><sub>B</sub>(Bayesian指数)分别为0.507和0.368,表现出高的遗传多样性。8个种群的遗传多样性呈现出随海拔升高而增大的趋势。AMOVA分析表明,74.3%的遗传变异存在于茶条槭种群内,25.7%存在于种群间(<i>Φ</i><sub>ST</sub>=0.257,<i>P</i><0.001)。相关回归分析显示,茶条槭种群的遗传多样性指数与土壤含水量、土壤全氮含量表现出显著的相关关系。8个种群可大致分成2组,与其地理分布相一致。茶条槭本身的生物学特性与不同海拔种群所处生境的异质性是七里峪茶条槭种群现有遗传格局的主要原因。

关键词: 茶条槭, 海拔梯度, 遗传多样性, 遗传结构, ISSR

Abstract:

To estimate genetic diversity and genetic structure of <i>Acer ginnala</i> at different elevations,eight natural populations in Qiliyu from 1 200 m to 2 000 m were surveyed using ISSR markers. At species level,a total of 78 reproducible DNA loci were yielded by 11 primers,and all of them were polymorphic,accounting for the percentage of polymorphic loci being 100%. Among the populations investigated,the total gene diversity <i>I</i>,<i>H</i><sub>B</sub> equaled 0.507 and 0.368 respectively,indicating a high genetic diversity occurred within <i>A. ginnala</i>. The amount of genetic variation of eight populations exhibited an increase along the elevation gradient. AMOVA showed that 25.7% of the total genetic variation existed among populations and 74.3% within populations (<i>Φ</i><sub>ST</sub>=0.257,<i>P</i><0.001). Regression analysis revealed significant relationships happened between the genetic diversity index and soil moisture content,soil total nitrogen respectively. The eight populations of <i>A. ginnala</i> were clustered into two groups based on PCoA and STRUCTURE analysis,which was consistent with their geographic distribution. The genetic variation pattern of <i>A. ginnala</i> in Qiliyu might be ascribed to heterogeneous habitats and biological characteristic of this species.

Key words: <, i>, Acer ginnala<, /i>, elevation gradient, genetic diversity, genetic structure, ISSR