关键词: 微量元素, 紫叶桃, 花色苷, 酶活性

Abstract:

The variation of anthocyanin and the relevant biosynthesis enzymes in Prunus persica f. atropurpurea leaves was investigated after spraying the leaves with microelement (Fe, Mn, Zn, B). The results indicated that the content of anthocyanins in leaves was not significantly different in each treatment in the beginning of treatments. The content of anthocyanin increased after 20 days with spraying Mn and Zn, and the anthocyanins content had extremely significantly positive correlation with Mn content. Fe inhibited synthesis of anthocyanin in P. persica f. atropurpurea leaves. Among phenylalanine ammonialyase(PAL), chalcone isomerase(CHI), dihydroflavonol 4-reductase (DFR) and UDP glucose flavonoid glucosyltransferase(UFGT), the change of DFR activity was more significant than PAL, CHI and UFGT, and the DFR activity after spraying Mn and Zn and the activity was significantly higher than CK. Moreover, the DFR activity increased before the anthocyanin content did, which suggested DFR initiated the synthesis of anthocyanins.

Key words: microelement, Prunus persica f. atropurpurea, anthocyanin, enzyme activity