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林业科学 ›› 2008, Vol. 44 ›› Issue (4): 35-40.doi: 10.11707/j.1001-7488.20080408

• 论文 • 上一篇    下一篇

水竹开花期间碳氮代谢特性*

王小红1.2 周祖基2   

  1. (1.成都大学旅游文化产业学院 成都610106;2.四川农业大学林学园艺学院 雅安625014)
  • 收稿日期:2007-02-02 修回日期:1900-01-01 出版日期:2008-04-25 发布日期:2008-04-25
  • 通讯作者: 周祖基

Characteristics of Carbon and Nitrogen Metabolism of Phyllostachys heteroclada at Flowering Stage

Wang Xiaohong1.2,Zhou Zuji2   

  1. (1.College of Tourism and Culture Industry, Chengdu University Chengdu 610106;2. College of Forestry and Horticulture, Sichuan Agricultural University Yaan 625014)
  • Received:2007-02-02 Revised:1900-01-01 Online:2008-04-25 Published:2008-04-25

摘要:

选取开花、未开花和开花后发生逆转的水竹作为研究试材,对其叶片中叶绿素、可溶性糖、淀粉、可溶性蛋白质、蛋白质总量及与碳氮代谢相关酶的活性变化规律行研究。结果表明:开花和开花逆转水竹叶片中叶绿素a、叶绿素b以及叶绿素总量有明显的波动,且表现出几乎相反的趋势,而未开花水竹这些指标无明显波动。开花水竹可溶性糖、总糖含量以及蔗糖酶活性在开花期间均呈比较明显的上升趋势,终值与平均值均远高于未开花和开花逆转水竹,同时其淀粉含量、淀粉酶和蔗糖酶活性在开花初期增加明显。开花水竹可溶性蛋白质含量和谷氨酰胺合成酶(GS)活性明显小于未开花和开花逆转水竹; 可溶性-可溶性蛋白质高于未开花和开花逆转竹,而其总糖/全氮无显著差异。总的来看,进入开花阶段以后,水竹碳代谢活动会增强而氮代谢会显著降低,这从一定程度上反映出碳氮代谢的强弱能预示植物从营养生长向生殖生长的转化。

关键词: 水竹, 开花, 碳氮代谢, 酶活性

Abstract:

The characteristics of flowering (type I), nonflowering(type II) and flowering reversion (type III) bamboo(Phyllostachys heteroclada), involving chlorophyll, soluble sugar, starch, protein content and key enzymes activity were studied. The results showed that chlorophyll a, chlorophyll b and total chlorophyll content of type I and type III were obviously unstable, comparing with type II. The change tendency of above mentioned indexes seemed almost opposite between type I and type III. Soluble sugar and total sugar contents, and activity of sucrase of type I increased, and the endingvalues and average values were more than those of the other two types. On the other hand, type I had fewer soluble protein content, less activity of GS, and more great soluble sugar/soluble protein than other two types, while the total sugar/total protein ratio was similar among the three. In general, the carbon metabolism increased and nitrogen metabolism decreased apparently at flowering stage of P. heteroclada, which suggested that the change of carbon and nitrogen metabolism could be a signal of the bamboo transition from growth to development. 

Key words: Phyllostachys heteroclada, flowering, carbon and nitrogen metabolism, activity of enzyme