褐马鸡圈养种群ISSR标记的遗传多样性

doi:10.11707/j.1001-7488.20131114

摘要/Abstract

摘要：

利用ISSR分子标记技术对褐马鸡庞泉沟国家自然保护区与太原市动物园2个种群的遗传多样性进行研究。从20个ISSR引物中筛选出10个引物对褐马鸡2个种群的35个样品进行扩增，得到65个清晰的扩增位点，多态带百分率（PPB）为76.9%。2个种群的多态带百分率和群体内遗传多样性指数分别为54.10%，54.10%，0.302 5，0.205 6，前者遗传多样性略高于后者。2个种群间的遗传分化系数（G_st）、基因流估计值（N_m）、遗传相似性系数以及遗传距离均表明种群间的遗传分化较小。褐马鸡UPGMA 系统树无明显的歧化，尚未表现出遗传趋异。褐马鸡2个种群间的遗传多样性指数比较接近，遗传多样性水平较低，核基因组与过去线粒体基因组遗传多样性的研究结果基本一致。

关键词: 褐马鸡, 濒危物种, 圈养, ISSR, 遗传多样性

Abstract:

Brown-eared Pheasant (Crossoptilon mantchuricum) is a critically endangered endemic species, which is listed in the national first-class protected birds in China. In this study, ISSR markers were used to investigate the genetic diversity of two populations of Brown-eared Pheasant in the Pangquangou Nature Reserve and Taiyuan Zoo. Ten primers out of 20 ISSR primers were selected for amplifying 35 samples of the two populations, and 65 clear amplification sites (PPB=76.9%) were obtained.The percentage of polymorphic bands of the two populations was around 54.10%.Shannon's Information Index of the two populations was 0.302 5 and 0.205 6, respectively. The generic heterogeneity (G_st), genetic flow (N_m), genetic similarity index indicated that there was no significant genetic differentiation between the two populations. Statistical analysis and cluster tree diagram consistently illustrated that the genetic diversity between the two populations was at a low level. Individuals in each population had close genetic relationship. The result in this study were consistent to another study we did with the mitochondrial genome genetic diversity.

Key words: Brown-eared Pheasant, endangered species, populations, ISSR, genetic diversity

中图分类号:

S718.63

武玉珍, 冯睿芝. 褐马鸡圈养种群ISSR标记的遗传多样性[J]. 林业科学, 2013, 49(11): 103-108.

Wu Yuzhen, Feng Ruizhi. ISSR Analysis of Genetic Diversity of Two Brown-Eared Pheasant (Crossoptilon mantchuricum) Populations[J]. Scientia Silvae Sinicae, 2013, 49(11): 103-108.

参考文献

Arcade A, Anselin F, Faivre Rampant P, et al. 2000. Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch. Theoretical and Applied Genetics, 100(2): 299-307.
Aytekin I, Ozdil F, Zulkadir U, et al. 2011. Evaluation of ISSR markers for genetic diversity analysis in Anatolian Water Buffaloes. Journal of the Science of Food and Agriculture, 91(11): 1957-1962.
Bai X J. 2004.ISSR fingerprint for Manchurian Tigers in captivity. Acta Theriologica Sinica, 24(1): 90-92(in Chinese).
Bai X J, Li H. 2001.Study on ISSR fingerprinting of broiler chicken divergently selected for very low density lipoprotein (VLDL).Journal of Jinlin Agricultural University, 23(1): 80-87(in Chinese).
Casasoli M,Mattioni C,Cherubini M,et al.2001.A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD,ISSR and isozyme markers.Theoretical and Applied Genetics, 102(8):1190-1199.
Curole J P,Kocher T D.1999.Mitogenomics:digging deeper with complete mitochondrial genomes. Trends in Ecology and Evolution, 14(10):394-398.
Fang D Q, Roose M L.1997.Identification of closely related citrus cultivars with inter-simple sequence repeats markers. Theoretical and Applied Genetics, 95(3): 408-417.
Gilbert J E, Lewis R V,Wilkinson M J, et al. 1999. Developing an appropriate strategy to assess genetic variability in plant germplasm collections.Theoretical and Applied Genetics, 98(6/7): 1125-1131.
Hizer S E,Dhar A K,Klimpel K R,et al.2002.RAPD markers as predictors of infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance in shrimp (Litopenaeus stylirostris).Genome, 45(1):1-7.
Huang J C, Sun M. 2000. Genetic diversity and relationship of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theoretical and Applied Genetics, 100(7):1050-1060.
Jin Z X,Li J M, Li Z H.2007.ISSR analysis of the genetic diversity of Torreya jackii Chun natural populations in Xianju County, Zhejiang Province. Journal of Beijing Forestry University, 29(1):53-58(in Chinese).
Kostia S,Ruohonen-Lehto M,Vainola R.2000.Phylogenetic information in inter-SINE and inter-SSR fingerprints of the Artiodactyla and evolution of the Bov-tA SINE. Heredity, 84(1):37-45.
Li J, Li M, Nie L, et al. 2007. Analysis of parents and their hybrids of Antheraea pernyi with ISSR technique. Science of Sericulture, 33 (1): 113-116(in Chinese).
Moritz C.1991.The origin and evolution of parthenogenesis in Heteronotia binoei (Gekkonidae):evidence for recent and localized origins of widespread clones. Genetics, 129(1):211-219.
Noroozi M,Hishamuddin O,Soon G T,et al.2011.Studies on the genetic variation of the green unicellular Alga Haematococcus pluvialis (Chlorophyceae) obtained from different geographical locations using ISSR and RAPD molecular marker. Molecules, 16(3): 2599-2608.
Ostberg C O,Rodriguez R J.2002.Novel molecular markers differentiate Oncorhynchus mykiss (rainbow trout and steelhead) and the O. clarki (cutthroat trout) subspecies. Molecular Ecology Notes, 2(3):197-202.
Parsaeian M, Mirlohi A, Saeidi G. 2011. Study of genetic variation in sesame (Sesamum indicum L.) using agro-morphological traits and ISSR markers. Russian Journal of Genetics, 47(3):314-321.
Pharmawati M, Yan G, Finnegan P M.2005.Molecular variation and fingerprinting of Leucadendron cultivars (Proteaceae) by ISSR markers. Annals of Botany, 95(7):1163-1170.
Sanker A A, Moore G A.2001.Evalution of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theoretical and Applied Genetics, 102(2):206-214.
Sica M,Gamba G,Montieri S,et al. 2005.ISSR markers show differentiation among Italian populations of Asparagus acutifolius L. BMC Genetics, 6(1):17.
Willis P M, Crespi B J, Dill L M,et al. 2004.Natural hybridization between Dall’s porpoises(Phocoenoides dalli)and harbour porpoise(Phocoena phocoena).Canadian Journal of Zoology, 82(5):828-834.
Wright S.1931. Evolution in Mendelian populations.Genetics, 16(2):97-159.
Wu Y Z, Wang M B, Zang F. 2010. D-loop sequence variation of Mitochondrial DNA in captive Brown-eared Pheasant (Crossoptilon mantchuricum).Acta Ecologica Sinica, 30(11): 2958-2964(in Chinese).
Yeh F C,Yang R C,Boyle T B J,et al. 1997. POPGENE Version 1.31,the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre,University of Alberta. Edmonton, Canada.
Zhao Q, Du H, Zhuang D H, et al. 2008. Genetic relationships analysis of 14 cultivars of Phalaenopsis hybrid based on ISSR molecular markers. Bulletin of Botanical Research, 28(2): 227-231 (in Chinese).
Zheng F, Lu X L, Sun H Y, et al. 2007. Application of ISSR markers in germplasm analyzing of mitten crab. Journal of Fishery Sciences of China, 14(1):46-51(in Chinese).
Zietkiwicz E, Rafalski A, Labuda D. 1994. Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2): 176-183.

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