关键词: 泡桐, 离体开花, 外植体, 培养基

Abstract:

In order to shorten the vegetative growth period and provide fine conditions for hybridization breeding of Paulownia plants, the effect of combinations of phytohormone and sucrose in the MS media and temperature on in vitro flowering of Paulownia australis, P. fortunei and P. elongata was investigated. The results indicated that the highest percentage of P. australis and P. fortunei in vitro flowering might reach 90% and 95% under the condition of 15 ℃ culture temperature, 50 g·L^-1 sucrose and MS+0.9 mg·L^-1 NAA+12 mg·L^-1 ZT or MS+1.2 mg·L^-1 NAA+10 mg·L^-1 ZT medium respectively, and that of P. elongata 93.3% under 25 ℃ culture temperature, 50 g·L^-1 surcose and MS+1.2 mg·L^-1 NAA+10 mg·L^-1 ZT medium.

Key words: Paulownia, in vitro flowering, explant, medium