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林业科学 ›› 2013, Vol. 49 ›› Issue (6): 30-36.doi: 10.11707/j.1001-7488.20130605

• 论文与研究报告 • 上一篇    下一篇

基于Illumina高通量测序的泡桐转录组研究

邓敏捷1,2, 董焱鹏1,2, 赵振利1,2, 张晓申1,3, 范国强1,2   

  1. 1. 河南农业大学泡桐研究所 郑州 450002;
    2. 河南农业大学林学院 郑州 450002;
    3. 郑州市农林科学研究所 郑州 451400
  • 收稿日期:2013-01-29 修回日期:2013-04-20 出版日期:2013-06-25 发布日期:2013-07-16
  • 通讯作者: 范国强
  • 基金资助:

    国家自然科学基金项目(30271082, 30571496);高等学校博士学科点专项科研基金项目(20050466003)和河南省杰出人才计划项目(122101110700)。

Illumina-Based de novo Sequencing and Characterization of the Transcriptome of Paulownia Plant

Deng Minjie1,2, Dong Yanpeng1,2, Zhao Zhenli1,2, Zhang Xiaoshen1,3, Fan Guoqiang1,2   

  1. 1. Institute of Paulownia, Henan Agricultural University Zhengzhou 450002;
    2. College of Forestry, Henan Agricultural University Zhengzhou 450002;
    3. Zhengzhou Research Institute of Agriculture and Forestry Sciences Zhengzhou 451400
  • Received:2013-01-29 Revised:2013-04-20 Online:2013-06-25 Published:2013-07-16

摘要:

利用新一代高通量测序技术平台Illumina Solexa对泡桐进行转录组测序和数据de novo组装,并对得到的unigene进行功能注释、分类及代谢通路分析。结果表明,N50值为2 278 bp、平均长度为1 410 bp的泡桐unigene共有188 019条,将其与NCBI的Nr和Swiss-Prot数据库比对后发现,分别有120 808条和85 880条unigene与其他物种的基因具有同源性。利用COG数据库可将有关的41 914条泡桐unigene分成25类,KEGG数据库分析发现共有43 553个unigene参与215种代谢通路。找到与木质素合成相关的泡桐unigene。

关键词: 泡桐, 转录组, Illumina高通量测序, 从头组装

Abstract:

The transcriptome Paulownia plant was sequenced by using Illumina technology, and the functional annotations and metabolic pathways analyses of some unigenes were conducted in this present paper. The results indicated that 188 019 unigenes with an average length of 1 410 bp and N50 value of 2 278 bp were obtained with de novo assembly. Comparison with NCBI and Swiss-Prot protein databases showed that 120 808 and 85 880 unigenes had homology with the genes from other species, respectively. The 41 914 unigenes were able to be assigned to 25 categories with the COG (Clusters of Orthologous Groups) database. Functional annotation against KEGG (Kyoto Encyclopedia of Genes and Genomes) database identified 43 553 unigenes which were mapped to 215 metabolic pathways. Moreover, some Paulownia unigenes related to lignin biosynthesis were found using this information platform. The result provides reference for future excavations of new genes, as well as screening and identifying miRNA of Paulownia plants.

Key words: Paulownia, transcriptome, Illumina sequencing, de novo assembly

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