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林业科学 ›› 2007, Vol. 43 ›› Issue (07): 148-151.doi: 10.11707/j.1001-7488.20070726

• 研究简报 • 上一篇    下一篇

不同提取条件对核桃叶中抗病毒物质活性的影响

翟梅枝 贾彩霞 景炳年 刘朝斌   

  1. 西北农林科技大学林学院,杨凌712100
  • 收稿日期:2006-01-10 修回日期:1900-01-01 出版日期:2007-07-25 发布日期:2007-07-25

Influence of Extract Condition on Activity of Antiviral Substances from Walnut Leaves

Zhai Meizhi,Jia Caixia,Jing Bingnian,Liu Chaobin   

  1. College of Forestry, Northwest A & F University Yangling 712100
  • Received:2006-01-10 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

关键词: 核桃, 烟草花叶病毒, 提取方法

Abstract:

The antiviral substances of walnut leaves were extracted by different solvents and methods, and its antiviral activities were determined. The results showed that antiviral substance activity is dissimilar based on extracts from walnut leaves with different solvent and methods. Inhibition rate on TMV of the extract with 95% (V/V) ethanol is the best, 100%; Optimum condition of the cold soak are: 95% (V/V) ethanol is used as solvent, the ratio of material to solvent is 20/100 (1∶10), soak time is 18 h. The inhibition rate of the extract to TMV infecting Nicotiana glutinosa is 100% under optimum condition.

Key words: walnut, tobacco moscaic virus (TMV), extracted method