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林业科学 ›› 2007, Vol. 43 ›› Issue (05): 64-68.doi: 10.11707/j.1001-7488.20070511

• 论文及研究报告 • 上一篇    下一篇

白僵菌和肿腿蜂对松墨天牛幼虫的作用

刘洪剑1,2 朴春根1 汪来发1 申相澈3 郑荣镇3 束庆龙2   

  1. 1.中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091;2.安徽农业大学林学与园林学院合肥230036;3.韩国山林科学院,首尔130-712
  • 收稿日期:2006-10-16 修回日期:1900-01-01 出版日期:2007-05-25 发布日期:2007-05-25

Biocontrol of Monochamus alternatus by Beauveria bassiana and Scleroderma guani

Liu Hongjian1,2,Piao Chungen1,Wang Laifa1,Shin Sangchul3,Chung Yeongjin3,Shu Qinglong2   

  1. 1.Key Laboratory of Forest Protection,State Forestry Administration Research Institute of Forest Ecology,Environment and Protection,CAF Beijing 100091;2.College of Forestry and Garden,Anhui Agricultural University Hefei 230036;3.Korea Forest Research Institute Seoul 130-712
  • Received:2006-10-16 Revised:1900-01-01 Online:2007-05-25 Published:2007-05-25

摘要:

在不同温度、不同剂量下用白僵菌侵染松墨天牛越冬4龄幼虫,结果表明:20~25℃是白僵菌作用松墨天牛的最适温度范围。20℃时3种浓度剂量的白僵菌孢悬液在松墨天牛体内有效继代校正宿存率均达最高,分别为58%、95%、100%。通过白僵菌、管氏肿腿蜂及携带白僵菌的管氏肿腿蜂分别对松墨天牛4龄幼虫的作用比较发现:第7天时,单独接种白僵菌的最高死亡率在25℃条件下是26.3%;单独接肿腿蜂的最高死亡率是55.0%;携菌肿腿蜂的最高致死率为94.4%。林间防治试验表明:肿腿蜂携带白僵菌使松墨天牛幼虫死亡率达到61.1%,而肿腿蜂使松墨天牛幼虫的林间致死率是40.8%,白僵菌的为0。肿腿蜂携带白僵菌可对松墨天牛幼虫起到更好的防治效果。

关键词: 白僵菌, 肿腿蜂, 松墨天牛, 致病力

Abstract:

By the infection experiment of Beauveria bassiana to the overwintering larvae of Monochamus alternatus under different temperatures and dosages,the results showed:20~25 ℃ was the fittest temperature range for B.bassiana to M.alternatus.Under 20 ℃,parasitic rates of B.bassiana to M.alternatus by three kinds of concentration of B.bassiana spore suspension were 58%、95% and 100% respectively.By comparing mortality of M.alternatus at 4 th instars after inoculation of B.bassiana,Scleroderma guani and S.guani with B.bassiana,on the 7 th day,the highest mortality of M.alternatus only inoculating B.bassiana was 26.3%;the highest mortality of M.alternatus only inoculating S.guani was 55.0%;and the highest montality of M.alternatus inoculating and S.guani with B.bassiana was 94.4%.The field experiment showed that the mortality of the M.alternatus larvae treated by S.guani with B.bassiana was 61.1%,by S.guani was 40.8%,and by B.bassiana was 0.0%.So S.guani with B.bassiana might have better effect to the larvae of M.alternatus.

Key words: Beauveria bassiana, Scleroderma guani, Monochamus alternatus, pathogenicity