欢迎访问林业科学,今天是

林业科学 ›› 2005, Vol. 41 ›› Issue (1): 205-210.doi: 10.11707/j.1001-7488.20050135

• 研究简报 • 上一篇    下一篇

生防因子螺旋毛壳ND35的细胞壁降解酶与重寄生作用

高克祥1 刘晓光2 Dana Friesem3 Leonid Chernin3 时呈奎2   

  1. 1.西北农林科技大学植物保护学院,杨凌712100;2.山东农业大学植物保护学院,泰安271018;3.希伯莱大学农业、食品与环境科学院植病和微生物系及农业生物技术中心,76100
  • 收稿日期:2003-10-09 修回日期:1900-01-01 出版日期:2005-01-25 发布日期:2005-01-25

Cell Wall Degrading Enzymes Involved in Mycoparasitism of the Biocontrol Agent Chaetomium spirale ND35

Gao Kexiang1,Liu Xiaoguang2,Dana Friesem3,Leonid Chernin3,Shi Chengkui2   

  1. 1.College of Plant Protection, Northwest Sci-Tech University of Agriculture and Forestry Yangling 712100; 2.College of Plant Protection, Shandong Agricultural University Taian 271018; 3.Department of plant Pathology and Microbiology, Otto-warburg center for Agricultural Biotechnology , Faculty of Agricultural, Food and Environmental Sciences,The Hebrew University of Jerusalem,PO Box 12, Rehovot 76100, Israel
  • Received:2003-10-09 Revised:1900-01-01 Online:2005-01-25 Published:2005-01-25

关键词: 螺旋毛壳ND35, 细胞壁降解酶, 活性检测, 重寄生, 扫描电镜

Abstract:

Some Chaetomium spp. Are capable of antagonizing several plant pathogenic fungi through production of antibiotics and mycoparasitism. Secretion of lytic enzymes, mainly including glucanases and chitinases, is considered the most important step in the mycoparasitic process. In this study, an about 110kDa exo-β-1,3-glucanase from C. spirale ND35 was detected both in culture filtrate and directly on PAGE and IEF gels, as well as chitinases, although protease was not detectable on Litmus milk agar plates. Coiling and penetrating the hyphae of host fungus Valsa mali were observed by scanning electron microscope (SEM), which may be related to the synergistic interaction between β-1,3-glucanase and chitinases. Β-1,3-glucanase activity of C. spirale ND35 varied considerably when C. spirale ND35 was grown in different carbon sources during various incubation time, and might be subjected to both induction by substrate and catabolite repression.

Key words: Chaetomium spirale ND35, cell wall degrading enzymes, assays of enzyme activities, mycoparasitism, scanning electron microscope