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林业科学 ›› 2005, Vol. 41 ›› Issue (1): 202-204.doi: 10.11707/j.1001-7488.20050134

• 研究简报 • 上一篇    下一篇

利用ISSR DNA标记鉴定主要银杏栽培品种

沈永宝1 施季森1 赵洪亮2   

  1. 1.南京林业大学国家林业局南方林木种子检验中心,南京210037;2.江苏省邳州市林业局,邳州221400
  • 收稿日期:2003-12-24 修回日期:1900-01-01 出版日期:2005-01-25 发布日期:2005-01-25

Identification on the Main Cultivated Varieties of Ginkgo biloba Using ISSR DNA Marker

Shen Yongbao1,Shi Jisen1,Zhao Hongliang2   

  1. 1.Nanjing Forestry University Southern Tree Seed Inspection Center Nanjing 210037; 2.Pizhou Forest Administration, Jiangsu Province Pizhou221400
  • Received:2003-12-24 Revised:1900-01-01 Online:2005-01-25 Published:2005-01-25

关键词: 银杏, ISSR标记, 鉴定

Abstract:

Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46-550,ISSR46-670,ISSR46-740,ISSR46-1000,ISSR46-1159,ISSR46-1359,ISSR46-1600,ISSR44-520,ISSR44-580,ISSR44-660,ISSR44-1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker.

Key words: Ginkgo biloba, ISSR marker, identification