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林业科学 ›› 2003, Vol. 39 ›› Issue (1): 56-61.doi: 10.11707/j.1001-7488.20030109

• 论文及研究报告 • 上一篇    下一篇

马占相思树对低温冻害的抗性研究

詹福建1 巫光宏2 黄卓烈3 罗焕亮4 孟立科5   

  1. 华南农业大学生命科学学院,广州510642;华南理工大学食品与生物工程学院,广州510641
  • 收稿日期:2000-10-23 修回日期:1900-01-01 出版日期:2003-01-25 发布日期:2003-01-25

STUDY ON THE RESISTANCE OF ACACIA MANGIUM TO LOW TEMPERATURE STRESS

Zhan Fujian1,Wu Guanghong2,Huang Zhuolie3,Meng Like5,Luo Huanliang4   

  1. College of Life Science,South China Agricultural University\ Guangzhou510642;College of Food and Biological Engineering,South China University of Technology\ Guangzhou510641
  • Received:2000-10-23 Revised:1900-01-01 Online:2003-01-25 Published:2003-01-25

摘要:

以盆栽马占相思(Acacia mangium)PNE17868家系幼苗为材料,研究了低温胁迫下树苗生长发育有关的生理生化指标的变化及其与抗冻害的关系。结果表明,随着低温胁迫程度的加深和时间延长,植株细胞电导率显著升高,细胞膜ATPase活性呈下降趋势,而可溶性糖、脯氨酸和可溶性蛋白质含量亦不断升高,表现出耐寒植物较典型的生理特点,说明该植株有明显的渗透调节能力和抗寒性。在零下低温(0~-6℃)胁迫下,植物细胞防御系统的保护酶类(CAT、POD、SOD)的活性先是升高,然后有所下降,但下降幅度不大。这表明了马占相思PNE17868家系对低温有较强的适应能力,在温度日益降低(0~-6℃)条件下体内保护酶仍能维持较高的活性水平,减轻了由膜脂过氧化引起的膜伤害,是植物提高抗寒性、免遭低温冻害的重要原因。

关键词: 马占相思, 抗寒性, 保护酶类

Abstract:

The relationship between the antichilling ability of the plants and their physiological changes was studied in Acacia mangium PNE 17868 clone suffered from temperature changes from 12℃ to -6℃.The results showed that the electrical conductivity of the cells increased obviously,and the activity of ATPase reduced.At the same time the contents of soluble sugar,proline,and protein increased.Under the temperature of 0~-6℃,the activities of the protective enzymes,I.e.catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD),increased firstly and then decreased slightly.However they still sustained in a relatively high level.This indicated that the plants of Acacia mangium PNE 17868 clone had a relatively high adaptivity to cold stress.

Key words: Acacia mangium, Antichilling ability, Protective enzymes