欢迎访问林业科学,今天是

林业科学 ›› 2014, Vol. 50 ›› Issue (7): 44-51.doi: 10.11707/j.1001-7488.20140707

• 论文与研究报告 • 上一篇    下一篇

杨树苗期抗寒性综合评价体系的构建

李晓宇, 杨成超, 彭建东, 杨志岩, 张妍   

  1. 辽宁省杨树研究所 盖州 115200
  • 收稿日期:2013-08-16 修回日期:2013-11-06 出版日期:2014-07-25 发布日期:2014-07-04
  • 基金资助:

    辽宁省科学事业公益研究基金(2011005008)。

Establishment of an Integrated Assessment System on Cold Resistance of Poplars at the Seedling Stage

Li Xiaoyu, Yang Chengchao, Peng Jiandong, Yang Zhiyan, Zhang Yan   

  1. Liaoning Provincial Research Institute of Poplar Gaizhou 115200
  • Received:2013-08-16 Revised:2013-11-06 Online:2014-07-25 Published:2014-07-04
  • Contact: 杨成超

摘要:

采用11个杨树无性系的1年生枝条为试验材料,通过低温和冻融处理,确定以4次冻融处理后(-40℃放置24 h然后转入20℃放置24 h)的枝条萌芽率为抗寒性的衡量指标。从生理生化物质、物理性状、生长性状、物候期等方面进行研究,用相关性分析筛选出与抗寒性极显著或显著相关的5个指标——相对电导率、相对含水率、水分饱和亏缺、丙二醛和可溶性糖,用主成分分析法将5个指标转化为综合的主成分,确定各指标权重,建立评价体系,再结合隶属度函数值求得各无性系抗寒性的综合指数。依照综合指数,抗寒性由强到弱的排序为:小黑杨、小美旱、中绥12、小叶杨、3016杨、108杨、沙兰杨、辽育3、97杨、中辽1号、L35,其结果与参试无性系苗期野外抗寒性表现相符。通过聚类分析,将杨树抗寒性划分为4类:小黑杨、小美旱、中绥12和小叶杨为一类,其抗寒性最强;3016杨、108杨和沙兰杨为一类,抗寒性较强;辽育3、97杨和中辽1号为一类,抗寒性较弱;L35为一类,其抗寒性最差。评价体系的建立为准确判断不同杨树无性系苗期抗寒能力提供技术支持,进而为抗寒品种选育、品种区划等提供参考。

关键词: 杨树, 抗寒性, 冻融处理, 综合评价, 生理生化指标

Abstract:

The germination rate of annual branches of 11 poplar (Populus) clones were used as a judging indicator to investigate the cold resistance after cold hardiness treatment with low-temperature and freezing-thawing at-40℃ for 24 hours and then transferred to 20℃ for 24 hours repeatedly four times. Five parameters, such as relative electric conductivity, relative water content, water saturation deficit, MDA and soluble sugar, were selected by using correlation analysis for studying physiological and biochemical substances, physical characters, growth traits and phenological phases etc. The parameters were highly significantly or significantly correlated to the hardiness. The five parameters were further converted to integrated index by principle component analysis, with the combination of their subordinate functions to establish an assessment system. Integrated index value of cold resistance of each variety was obtained by measuring the weight and its function value. According to composite index, the sequence of cold resistance from strong to weak was P.×xiaohei, P. ×xiaozhuanica ‘Poplar’, P. deltoides ×(P. nigra var. thevestina ×P. nigra), P. simonii, P. ×canadensis ‘N3016’, P. ×canadensis ‘Guariento’, P. ×canadensis ‘Sacran-79’, P. ×deltoides ‘Liaoyu3’, P. ×euramericana ‘97’, P. ×euramericana ‘Zhongliao1’, P. ×euramericana ‘L35’. The result was consistent with the performance in cold resistance of the clones in field. These clones of poplars could be divided into four groups by cluster analysis. The first group, the first 4 clones in the resistance sequence, had the strongest cold resistance. The second group, including the 5-7th in the sequence, had medium cold resistance. The third group, including the 8-10th in the sequence, had relative weak resistance. The last one in the sequence was the worst. The evaluation system provided a technical support to judge the ability of cold resistance of seedlings of different clones, and would be beneficial to others such as breeding for cold resistance, varieties classification.

Key words: poplar, cold resistance, freezing and thawing, integrated assessment, physiological and biochemical indexes

中图分类号: