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林业科学 ›› 2002, Vol. 38 ›› Issue (1): 150-153.doi: 10.11707/j.1001-7488.20020124

• 研究简报 • 上一篇    下一篇

RAPD标记在鹅掌楸属中的遗传研究

李周岐 王章荣   

  1. 西北农林科技大学,杨凌712100;南京林业大学,南京210037
  • 收稿日期:2001-04-09 修回日期:1900-01-01 出版日期:2002-01-25 发布日期:2002-01-25

GENETIC STUDY OF RAPD MARKERS IN LIRIODENDRON

Li Zhouqi,Wang Zhangrong   

  1. Northwest Sci Tech University of Agriculture and Forestry Yangling 712100;Nanjing Forestry University Nanjing 210037
  • Received:2001-04-09 Revised:1900-01-01 Online:2002-01-25 Published:2002-01-25

关键词: RAPD, 遗传, 鹅掌楸属

Abstract:

Fourteen 10 mer primers were used for RAPD (Random amplified polymorphic DNA) analysis across a fullsib family, including 2 parents and 18 sibs, of Liriodendron chinense×L. Tulipifera. Of the total 57 fragments amplified, 20 (35.09%) were polymorphic between the 2 parents and 28 (49.12%) segregants in the sibs, indicting a little lower of polymorphism and heterozygosity at RAPD loci in the 2 species, compared with other tree species studied. Of the 20 polymorphic loci, 4 (appeared in the male parent) did not segregate in the progeny, indicating the genotype in these loci were AA (α=0.518), so, may be used as paternal markers for paternal identification. Fourteen parental polymorphic loci (24.56%) segregated 1∶1 in the progeny as expected, and 12 parental monomorphic loci (21.05%) segregated 3∶1 as expected, showing a Mendelian dominant inheritance fashion for an allele. Two parental monomorphic loci (3.51%) were detected to segregate in aberration from the expected ratios (3∶1).

Key words: RAPD, Inheritance, Liriodendron