关键词: 落叶松, 磷胁迫, 根际, pH值, 有效磷

Abstract:

Phosphorus is an important element for larch (Larix gmelinii Rupr.)root development.P-availability in rhizosphere is close with root exudates or excretions,e.g.,organic acid exudation,proton excretion.These materials from roots will affect pH value of rhizosphere.The aim of this paper were to study on pH changes at different P-stress conditions,and to test on the relation between available P and pH in rhizosphere.This work was carried out under pot experiment and field soil smaple analysis.Larch seedlings,1 and 2-year-old,were planted into sand pots.Nutrient solution with different P contents (0,0.035,0.071 and 0.142g·L^-1 respectively) were supplied everyday.There were 2 replicates for 1-year-old seedlings,5 replicates for 2-year-old seedlings at ecch P-level treatment.After 1-year-old seedlings growing for 40 days and 2-year-old seedlings growing for 90 days,seedlings were taken out and used to visualize acidification by means of an agar plate technique.The result showed that pH of rhizosphere decreased from 6.40 to 4.73～4.84 in 1-year-old seedlings and to 5.30～5.50 in 2-year-old after 36h incubation.Average pH decreased were 1.56～1.67 for 1-year-old seedlings,and 0.90～1.10 for 2-year-old seedlings.There was not significant,for pH variations in rhizosphere,among four kind treatments.The pH change was not affected by P-level.The result of soil sample analysis,from 12-year-old,21-year-old,and 40-year-old pure larch stands respectively,indicated that the pH changes in rhizosphere is 0.11,0.04,and 0.09,respectively,lower than that in the non-rhizosphere,acidification is not significant,While the available P in the rhizosphere is 18.1%,86.5% and 23.4%,higher than that in the non-rhizosphere in three stands.The correlation between pH variations and available P was not remarkable at this analysis.This means that there was no relationship between the available P and pH change of rhizosphere in larch root system.

Key words: Larix gmelinii Rupr., P-stress, Rhizosphere, pH, Available-P