Loading...
Welcome to visit Scientia Silvae Sinicae,Today is

Table of Content

25 November 2016, Volume 52 Issue 11
Relationship between Spatial Distribution of Aboveground Biomass of Desert Vegetation and Groundwater Depth in the Lower Reaches of Tarim River, Xinjiang, China
Bai Yufeng, Chen Chaoqun, Xu Hailiang, Zhang Guangpeng, Zhang Pei, Ling Hongbo
2016, 52(11):  1-10.  doi:10.11707/j.1001-7488.20161101
Abstract ( 219 )   HTML   PDF (607KB) ( 633 )  
References | Related Articles | Metrics
[Objective] The spatial distribution of aboveground biomass of desert vegetation along with the distance away from riverway in combination with groundwater depth were studied to provide a theoretical basis for restoration and rehabilitation of the ecological environment in arid desert region.[Method] A transect consisting of 20 continuous sample plots (size of 100 m×100 m) was set in September, 2014 at Yingsu section in the lower reaches of TarimRiver. Plant species and total number of trees and shrubs within sample plots together with crown size, plant height and DBH were recorded. According to the biomass model of Populus euphratica and the fresh weight of standard shrub branches, the aboveground biomass was calculated respectively. The sample plots were further divided into four smaller plots (size of 50 m×50 m), and then 6 to 10 even smaller sample plots (size of 1 m×1 m) along the diagonal were randomly selected, the total herbaceous plant species and numbers in each sample plot were recorded, all grasses were harvested to the ground surface to get aboveground biomass. Finally, a fitted function was created according to the relationship between aboveground biomass and groundwater depth, and then the regression equations and regression coefficients were tested for significance.[Result] The results showed that total aboveground biomass, tree aboveground biomass and shrub aboveground biomass were all negatively correlated with the distance to the riverway and with the groundwater depth (P<0.05). Aboveground biomass of shrub firstly increased and then decreased consistently, showing a single peak type. The total aboveground biomass of shrub and herbaceous plants reached 855.06 g·m-2 when groundwater depth was less than 4 m (0 to 300 m away from the riverway); and 62.45% was reduced (321.08 g·m-2) when groundwater depth was in range of 4 to 5.5 m (300 to 1 000 m away from the riverway), and 87.11% was reduced (110.19 g·m-2) when groundwater depth was above 5.5 m (1 000 to 2 000 m to the riverway).[Conclusion] Community structure and aboveground biomass of desert plants varied with the distance to the river and groundwater depth, indicating that groundwater depth significantly affected the spatial distribution pattern of desert vegetation and aboveground biomass. The controlling mechanism in the ecosystem of extreme arid area is quite unique because as the main water source, river in the desert determines the spatial distribution pattern and structure of the desert vegetation.
Effects of Litter Manipulation on Soil Respiration in the Natural Forests and Plantations of Castanopsis carlesii in Mid-Subtropical China
Li Wei, Liu Xiaofei, Chen Guangshui, Zhao Benjia, Qiu Xi, Yang Yusheng
2016, 52(11):  11-18.  doi:10.11707/j.1001-7488.20161102
Abstract ( 271 )   HTML   PDF (656KB) ( 697 )  
References | Related Articles | Metrics
[Objective] We investigated the effects of litter manipulation on soil respiration in Castanopsis carlesii forest and C. carlesii plantation to provide a scientific basis for an estimate of carbon dioxide emission in forest soil for the global climate change in the future.[Method] Our experimental sites are located at the C. kawakamii Nature Reserve, Sanming, Fujian, China, where a C. carlesii forest (less man-made interference in recent 200 years) and a C. carlesii plantation (planted 50 years ago) were selected. We set 3 plots (20 m×20 m) in each forest. And each plot was set 9 subplots (1 m×1 m). A litter manipulation experiment (No Litter, NL; Double Litter, DL and Control, CK) was carried out in these subplots. Soil respiration was measured using a soil carbon flux system (LI-8100A) during the period from January 2013 to December 2014. Soil temperature and moisture were also monitored at the same time.[Result] The mean soil temperature of C. carlesii forest in NL, DL, and CK were 18.2, 18.05, and 17.98℃, respectively. And the mean soil temperature of C. carlesii plantation in NL, DL, and CK were 18.68, 18.70, and 18.76℃, respectively. The results showed that soil temperature did not change significantly among different sample plots. However, the soil temperature was significantly larger in plantation than in natural forest.Litter respiration increased significantly from 11.8% to 29.7% in C. carlesii plantation. DL significantly increased soil respiration by 25.2%(P<0.05)in C. carlesii forest, indicating priming effects. But there were no significant change in C. carlesii plantation with DL treatment, which had no priming effects. A very significant exponential relationship was founded between soil respiration and soil temperature at 5 cm soil depth in litter manipulation plots (P<0.001), while a significant linear relationship was found between soil respiration and soil moisture at 0-10 cm depth in all study plots (P<0.05).[Conclusion] The results suggested that increased litterin natural forest of Castanopsis carlesii least to priming effect on the soil respiration, but no significant impacts on soil respiration of plantations.
Correlation between Terpenes Emission from Typical Forest Tree Species and Environmental Elements in Temperate Zone
Hua Shengzhou, Chen Jungang, Yu Xinxiao, Bi Huaxing, Fu Yanlin, Chen Jing, Sun Fengbin
2016, 52(11):  19-28.  doi:10.11707/j.1001-7488.20161103
Abstract ( 281 )   HTML   PDF (1162KB) ( 751 )  
References | Related Articles | Metrics
[Objective] Determining the emission types, relative content and rate of terpene compounds from plant source could provide the theoretical basis for establishing volatile organic compounds (VOCs) composition library of plant source and optimal deployment of urban forest.[Method] Half-closed cycling air collection model was used with Li-6400 portable photosynthesis system,Tenax absorption tube and gas pump together to collect plant volatile organic compounds in Beijing Jiufeng National Forest Park,and used to explore the differences of biogenic VOCs emission emitted by different tree species (coniferous species: Pinus tabulaeformis, Platycladus orientalis; broad-leaved species: Populus tomentosa, Quercus variabilis, Acer mono) and to analyze the relations between the VOCs emission and environmental and physiological factors.[Result] All the 5 typical forest tree species emitted alkanes, olefine, polycyclic aromatic hydrocarbons and halogenated-hydrocarbons. From the aspect of volatile relative content, Platycladus orientalis and Pinus tabulaeformis emitted mainly monoterpene, to which alpha-pinene contributed about 34.16% and 25.05% respectively. Populus tomentosa, Q.variabilis and A.mono mainly emitted isoprene, accounting for 76.47%, 55.25% and 32.61% respectively of the total emission. As for the diurnal variation pattern, isoprene emission from the 5 tree species displayed a single-peak distribution with the peak appeared between 11:00 and 12:00. The peak value of monoterpene was between 10:00 and 13:00, and valley value was between 17:00 and 18:00. The VOCs emission rate of the unit leaf area of Populus tomentosa was the largest, reaching 52.81 nmol·m-2 s-1, followed by Pinus tabulaeformis. The emission rate of alpha-pinene and cinene of Platycladus orientalis were the largest, up to 4.16 nmol·m-2 s-1 and 3.82 nmol·m-2 s-1 respectively. The emission rate of myrcene and alpha-pinene of Pinus tabulaeformis were large, up to 22.14 nmol·m-2 s-1 and 10.3 nmol·m-2 s-1 respectively. Overall, the isoprene emission rate of unit leaf area of broad-leaved species was higher, while the monoterpene emission rate of unit leaf area of coniferous species was higher. During the period of monitoring, the relative content of terpene was significantly positively correlated with temperature and photosynthetic intensity, but negatively correlated with relative humidity. The relative content of terpene was significanly positvely correlated with the net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration and transpiration rate.[Conclusion] Broad-leaved trees emit more isoprene, coniferous tree species emit more monoterpene. The relative content of terpene emitted by plant was increased with the temperature and photosynthetic intensity and reduced with relative humidity. The variation of net photosynthetic rate, stomatal conductance and transpisration rate showed a great consistence with the relative content of terpene from tree species.
Reasonable Canopy Light Intensity and High Light Efficiency Regulation of Sapindus mukorossi
Gao Yuan, Jia Liming, Gao Shilun, Su Shuchai, Duan Jie, Weng Zhen
2016, 52(11):  29-38.  doi:10.11707/j.1001-7488.20161104
Abstract ( 261 )   HTML   PDF (1148KB) ( 474 )  
References | Related Articles | Metrics

[Objective] In this study, the tree control technology was applied in order to solve low yield of Sapindus mukorossi biodiesel feedstock forest. This paper is focus on revealing tree canopy with improper light intensity, and hence provides the theoretical and technical support.[Method] The light illumination in different parts of naturally growing trees was measured for 2 years. The number of flowers and fruit yield index, controlling opening angle and number of backbone branches, thinning out of fruiting-shoot were measured and two-factor interaction validation was conducted in this research.[Rusult] 1) The light intensity of naturally growing trees were unreasonable—the interior canopy light intensity was only 10% to 15% of the space's. A model was established between light intensity and yield after regulating the canopy structure, y=-0.011 9x2+5.926 3x-565.37(R2=0.738 38) and the best light intensity could range from (200-270)×100 lx. 2) The main phenological phases could also be inferred according to double peaks of light intensity of the naturally growing trees' canopy. In May, at the reproductive growth prophase, trees badly need nutrition, and form the first peak of light intensity. In June, in the turning process from the leaves-sprout stage to the fruit-set stage, the light intensity becomes to a lower level. In August, with leaves falling gradually, light intensity increases to the second peak and then enters into a relatively stable phase. 3) Trimming and pruning operations could be determined by different light distribution proportion. Generally speaking, compared with the space's light intensity, exterior canopy's is 40%-54%, central canopy's is 32%-35%, and interior canopy's is 27%-31% after pruning, which may increase the yield by two or three times and make use of light effectively. 4)The experiment was conducted by setting up different backbone branches numbers (3, 4, 5), opening angles (30°,45°,60°,90°) and leaving fruiting branches (8-20). The experimental results showed that the radial distribution of three backbone branches and five backbone branches was able to effectively improve the light by two or three times compared to control. The yield and light intensity of four backbone branches were lowest. The yield of five backbone branches declined at a rate of 25% year by year. However the yield of three backbone branches showed a steady-state trend for several years. As for opening angles, the yield of the 90° treatment was highest in 2013 but decreased in a rate of 50% after first year, suggesting a premature aging phenomenon. With the treatment of 45° opening angle for three years, the annual yield was lowest. The 60° opening angle treatment had stable yield and light intensity and was the best among the all treatments. As for the fruiting branches, the treatment of 16-18 fruiting branches contributed to the highest yield of 347 g[DK]·m-2.[Conclusion] In general,three backbone branches, 60° opening angle and leaving 16-18 fruiting branches per m2 crown projection area could most effectively improve light intensity and the yield by one or two times, which is consistent with the results of two-factor interaction validation. The findings suggest that standardizing, gardening, intensive cultivation techniques system should be all combined with selecting excellent-genetic S.mukorossi and highly efficient and intensive techniques, so as to form an integrate tree management technology to enhance feedstock forest's production.

Growth Characteristics and Leaf Ultrastructures of Evergreen Poplar Clone Under Aluminum Stress
Qian Lianwen, Wu Wenjie, Sun Jingwei, Feng Ying
2016, 52(11):  39-46.  doi:10.11707/j.1001-7488.20161105
Abstract ( 216 )   HTML   PDF (3250KB) ( 504 )  
References | Related Articles | Metrics
[Objective] Poplar are a sort of plants that are usually for planting in neutral to partially alkaline soil, however the fast development of new varieties for cultivation has expanded its planting range. A-61/186(Populus × euramericana cv.A-61/186) is a evergreen poplar clone that is suitable for low-latitude tropical and subtropical areas, and it has been introduced to Fujian Province of China and presents evergreen characteristics. However, when it is planted in tropical or subtropical areas, it faces a prominent issue of acidic soil in which aluminum is rich, which can bring adverse effects. In this study, the patterns of aluminum accumulation and distribution in the body, the response of leaf ultrastructure and the physiological indexes under the condition of acid aluminum were investigated. The study aims to reveal mechanism of the macro and micro structure of the evergreen Poplar adapting to aluminum stress, thus providing a theoretical basis for the widespread of the evergreen poplar in subtropical regions. [Method] In this study, and the evergreen poplar clone ‘A-61/186’ was grown in hydroponic solution for aluminum stress experiment, and the effects of aluminum stress on the growth, biomass, chlorophyll fluorescence indicator and leaf ultrastructures by using transmission electron microscopy(TEM) were studied systematically. [Result] The results showed that the acidic aluminum stress had an impact on the growth of the evergreen poplar; the acidic aluminum stress significantly decreased the growth of roots, stems, and leaves as well as biomass of the evergreen poplar, and it had a much greater impact on the underground part than the above ground part. In addition, aluminum was accumulated more readily in the roots than in the stems and leaf tissues. Chlorophyll fluorescence showed that the absorbed light was non-photochemical and dissipated under aluminum stress, which reduced its photosynthetic efficiency, thereby affecting the growth and biomass. Furthermore, the degree of injury to the mesophyll cell ultrastructure is consistent with the growth and chlorophyll fluorescence parameters, which indicated that the effects of aluminum stress on the evergreen poplar clone's growth and biomass were directly related to the damage to the photosynthetic system.[Conclusion] With regards to the aluminum content in the evergreen poplar clone and the aluminum concentration that inhibited growth, the evergreen poplar clone was more sensitive than acidic species, such as tea, longan, and eucalyptus. Therefore, when planting this evergreen poplar clone in acidic soil, aluminum toxicity should be considered.
Accumulation and Allocation of Copper-Carbon Core-Shell Nanoparticles in Three Species
Wang Anke, Bi Yufang, Wang Yukui, Cai Hanjiang, Zhai Zhizhong, Zhong Hao, Du Xuhua, Ding Xingcui, Tian Xinli
2016, 52(11):  47-54.  doi:10.11707/j.1001-7488.20161106
Abstract ( 194 )   HTML   PDF (2220KB) ( 431 )  
References | Related Articles | Metrics
[Objective] CCCSNs is a new renewable composite nano-material that is called as the Copper-Carbon Core-Shell Nanoparticles, and it is fabricated by using cotton fibers as the template, with copper atoms homogeneously embedded in carbon black as a composite material. The special carbon shells can protect the copper core from oxidation, by which the Cu shows the typical antibacterial, antifungal, antifouling properties. This paper is expected to explore the the accumulation and dispersion of CCCSNs in Phyllostachys edulis, Dracaena sanderiana and Gossypium hirsutum, in order to provide the basis for the plants to make use of CCCSNs in promoting the anti-corrosion ability. [Method] The P. edulis and D. sanderiana were grown in hydroponic media with adding 0, 0.1 or 0.5 g L-1 CCCSNs. G. hirsutum was grown in potting soil amended with 0, 0.1 or 0.5 g CCCSNs per a pot, and watered with tap water. The three plant species were managed regularly. After 50 days, we harvested all samples that were used to test the copper concentration which could be considered as CCCSNs signal. The samples were dissolved by hydrogen nitrate and the extract was measured with atomic absorption spectrum. The anatomy of roots was observed by TEM and DES. [Results] CCCSNs was able to enter a plant and transport from roots to stems and leaves, and the accumulation of CCCSNs was dependent on the different species. The Cu concentration of P. edulis stems and leaves, when their roots exposed to 0.1 and 0.5 g·L-1 CCCSNs, was 13.19 and 11.79 μg·g-1, and were 263% and 225% higher than that of the control, respectively. With 0.1 and 0.5 g·L-1 CCCSNs treatment, the copper concentration of D. sanderiana stems and leaves was 29.31 and 27.95 μg·g-1 and 104% and 90% higher than that of the control, respectively. The Cu concentration in stems and leaves of G. hirsutum treated with 0.1 and 0.5 g·pot-1 was 5.22 and 6.53 μg·g-1and 24% and 52% higher than that of the control. The highest Cu concentration in stems and leaves of P. edulis, D. sanderiana and G. hirsutum was 21.65, 44.88 and 9.19 μg·g-1, respectively. D. sanderiana had the highest average Cu concentration and highest Cu concentration among the three species. However, P. edulis had the highest increasing rate of copper among the three species. Confirmed with TEM and DES detection, CCCSNs was able to accumulate in the cytoplasm, cell membrane, cell wall and intercellular space of root cells. Moreover, there was no difference in CCCSNs distribution pattern in the root cells over the three species. [Conclusion] When adding CCCSNs into the culture media of P. edulis, D. sanderiana and G. hirsutum, CCCSNs could enter the stems and leaves from the roots. The transportation capacity was dependent on the species. The Cu concentration in the plant was positively correlated with quantity of CCCSNs when the added CCCSNs was at a low level. Meanwhile, CCCSNs was able to accumulate in the cytoplasm, cell membrane, cell wall and intercellular space of root cells. Although the experiment had proved that CCCSNs could enter plants from roots, how CCCSNs enters the cells from intercellular space and in which way enters stems or other issues still need further research.
Characteristics of Soil Ammonia Volatilization in Fertilized Moso Bamboo (Phyllostachys edulis) Forests
Zhao Jiancheng, Su Wenhui, Fan Shaohui, Cai Chunju, Zhu Xiaowu, Liu Guanglu
2016, 52(11):  55-62.  doi:10.11707/j.1001-7488.20161107
Abstract ( 233 )   HTML   PDF (956KB) ( 461 )  
References | Related Articles | Metrics
[Objective] Nitrogen (N) is the greatest demand element for Moso bamboo (Phyllostachys edulis) growth. Improper fertilization could result in the decrease of nitrogen use efficiency (NUE) and the increase of nitrogen loss. Ammonia (NH3) volatilization is one of the main pathways of nitrogen loss from soil. Improper depth of fertilizer application in the soil could accelerate the loss of NH3 volatilization. However, very little information is available regarding NH3 volatilization and its influence factors in Moso bamboo forests. Thus, in this study the NH3 volatilization characteristics at different fertilization depths in a Moso bamboo forest were investigated by field experiment to provide the scientific guidance for the reasonable fertilization depth in terms of reducing the ammonia volatilization. [Method] In this paper, seven fertilization depths of 0, 5, 10, 15, 20, 25 and 30 cm and a control treatment without fertilizer were set in Huangshan, Anhui Province of China. The NH3 volatilized from each plot was absorbed by 2% H3BO3 with the venting method. One-way ANOVA was conducted to test the significance of the indexes, and the least significant difference (LSD) was applied for multiple comparisons. [Result] The NH4+ content after fertilization increased first and then declined with the extension of time, and finally stabilized. The NH3 volatilization showed obvious regularity changes with time, and it was closely related to fertilization depth. NH3 volatilization was detected on the second day after fertilization. The NH3 volatilization rates of all fertilization treatments increased first and then decreased, showed a single peak curve. NH3 volatilization rates at fertilization depths of 0, 5 and 10 cm peaked on the third day, while rates of NH3 volatilization at fertilization depths of 15, 20, 25 and 30 cm reached a maximum in 6 days after fertilization. Then, the rates decreased gradually. About twelve days later, NH3 volatilization rates dropped to a low level similar to the control. NH3 volatilization of urea could be divided into 2 stages, namely, rapidly and slowly increasing stages. The first eight days were recognized as the rapidly increasing stage. The ratios of NH3 volatilized within the first eight days to the total volatilization loss were ranged from 81.93% to 92.38%. Then it changed to the slowly increasing stage. The relationship between the cumulative NH3 volatilization (y) and the corresponding time (t) accorded with the Elovich equation (y=a + b lnt). By the end of this experiment, the losses of NH3 volatilization from the fertilization treatments were ranged from 10.12 to 27.17 kg·hm-2. Results showed that NH3 volatilization was influenced significantly by fertilization depth. The mean NH3 volatilization flux and total loss decreased with the increase of fertilization depth. NH3 volatilization losses of fertilization placed at 0 and 5 cm depths were larger than that at other depths, up to 27.17 and 25.66 kg·hm-2, equivalent to 21.05% and 19.88% of the applied N, respectively. When fertilization depth exceeded 15 cm, the loss ratio was significantly reduced. [Conclusion] In this study, NH3 volatilization was characterized by a high speed within the first eight days after fertilization in an intensive management bamboo forest. Thus, the regulation of environmental conditions was suggested to reduce N loss. The nitrogen losses through NH3 volatilization of fertilization placed below 15 cm depth were lower, however deep fertilization application could result in leaching loss and also increased the production cost. Comprehensive consideration of the distribution of bamboo root and nitrogen loss and utilization, the reasonable fertilization depth should be 15-20 cm in P.edulis forests.
A Comprehensive Evaluation of Kernel Quality under Agroforestry Models of Ginkgo biloba Plantation for Nut Production
Chen Lei, Sun Bing, Wang Guibin, Cao Fuliang, Feng Chaonian
2016, 52(11):  63-70.  doi:10.11707/j.1001-7488.20161108
Abstract ( 240 )   HTML   PDF (1356KB) ( 449 )  
References | Related Articles | Metrics
[Objective] The quality of Ginkgo(Ginkgo biloba) kernel from different agroforestry systems was studied to explore variations of seed yield and properties, kernel nutrients and secondary metabolites among different agroforestry systems,in order to provide a reference for application of different agroforestry systems in Ginkgo trees for nut production. [Method] Five traditional Ginkgo agroforestry systems (Ginkgo-Cole (Brassica campestris)-Peanut (Arachis hypogaea), Ginkgo-Wheat (Triticum aestivum)-Peanut, Ginkgo-Broadbean (Vicia faba)-Peanut, Ginkgo-Mulberry (Morus alba), Ginkgo-Cole-Corn (Zea mays)) and a control system (the pure Ginkgo stand) were chosen, and seed yield and mass, nut mass, kernel mass, and content of starch, protein, fat, soluble sugar, total flavonoids, terpene lactones, ginkgoic acid, HCN were measured to evaluate the quality of Ginkgo kernels using an improved analytic hierarchy process. [Result] Significant differences were observed in Ginkgo seed yield, nut mass, and contents of starch, protein, soluble sugar, total flavonoids, terpene lactones, ginkgoic acid, HCN among different systems (P<0.05). The Ginkgo seed yield, nut mass, contents of protein and soluble sugar were higher in the agroforestry systems than in the pure Ginkgo stand. The highest Ginkgo seed yield was found in Ginkgo-Mulberry system with 31.07 kg per tree, and Ginkgo-Broadbean-Peanut system had the highest nut mass, and contents of protein and soluble sugar, reaching 2.34 g, 101.73 mg·g-1, 88.11 mg·g-1, respectively. Higher contents of starch (0.55 g·g-1), total flavonoids (2.69 mg·g-1), terpene lactones (7.32 mg·g-1), ginkgoic acid (47.54 μg·g-1), HCN (0.44 μg·g-1) were found in the pure Ginkgo stand than in Ginkgo agroforestry systems. The lowest total flavonoids, ginkgoic acid, and HCN were found in Ginkgo-Cole-Peanut system, only reaching 1.84 mg·g-1, 28.52 μg·g-1and 0.27 μg·g-1, respectively, and the lowest content of terpene lactones was found in Ginkgo-Broadbean-Peanut system with a value of 4.82 mg·g-1. The improved analytic hierarchy process showed that the content of HCN was the most impacting factor for Ginkgo kernel quality, and its weight reached 0.210 3, followed by protein, soluble sugar, total flavonoids, ginkgoic acid, kernel mass, starch, terpene lactones, seed mass, and nut mass. Further calculation of Ginkgo kernel quality indicator showed that Ginkgo agroforestry systems had better Ginkgo kernel quality indicator than pure Ginkgo stand, and Ginkgo kernel quality in Ginkgo-Broadbean-Peanut system was the best with 0.414 8, followed by Ginkgo-Wheat-Peanut, Ginkgo-Mulberry, Ginkgo-Cole-Peanut, and Ginkgo-Cole-Corn.[Conclusion] The quality of Ginkgo kernel was improved in agroforestry system; it can be applied in Ginkgo trees for nut production, especially in Ginkgo-Broadbean-Peanut system which is suitable for promotion and application.
Development of SSR Markers Based on Transcriptome Sequence of Phoebe zhennan
Shi Xiaodong, Zhu Xuehui, Sheng Yuzhen, Zhuang Guoqing, Chen Fang
2016, 52(11):  71-78.  doi:10.11707/j.1001-7488.20161109
Abstract ( 294 )   HTML   PDF (1189KB) ( 644 )  
References | Related Articles | Metrics
[Objective] SSR (simple sequence repeat) markers is a very useful method for species identification, molecular linkage mapping, and genetic diversity studies. In order to develop molecular markers of Phoebe zhennan, SSR markers were developed based on transcriptome sequence. This study provided a basis for studies of genetic diversity and conservation of genetic resources of P. zhennan. [Method] A total of 67 331 unigenes obtained by transcriptome sequencing were used to develop SSRs. And it was feasible to develop molecular markers of P. zhennan by primers analysis.[Result] A total of 9 405 SSRs were identified through software analysis, at a frequency of 13.97%. Meanwhile, the number of sequences involved in these SSRs was 6 667, accounting for 9.90% of the total number of sequences. A total of 166 repeat types of SSRs were identified. Among them, mononucleotide, dinucleotide and trinucleotide were the dominant repeat types, and the number of SSRs was 3 890 (41.36%), 2 885 (30.68%), 2 489 (26.46%), respectively. SSR repeat frequencies were largely different, the largest number of repeats was 10, accounting for 17.24% of the total repeat types, followed by 5 repeats (16.47%) and 6 repeats (15.90%). Additionally, the dominant repeat type was A/T, AG/CT and AAG/CTT, and the distribution frequency was 40.67%, 27.59% and 11.29%. The results indicated the feasibility of developing SSR markers for P. zhennan by polymorphism analysis. Meanwhile, a total of 18 primer pairs were randomly selected and verified by PCR amplification, and 9 primer pairs of them were able to amplify expected products. [Conclusion] A total of 9 405 SSRs were identified from transcriptome sequencing of P. zhennan, with A/T, AG/CT and AAG/CTT the most common repeats. 18 primer pairs were chosen for verification through their amplification in P. zhennan. The SSR markers developed for P. zhennan will benefit studies in candidate gene mining, marker-assisted breeding and resources protection.
Functions of PeIRX10 Gene from Phyllostachys edulis in Xylan Synthesis
Wang Jie, Li Jun, Tong Tingting, Shao Xiangjun, Song Lili, Wu Aimin
2016, 52(11):  79-87.  doi:10.11707/j.1001-7488.20161110
Abstract ( 280 )   HTML   PDF (2288KB) ( 396 )  
References | Related Articles | Metrics
[Objective] PeIRX10 gene was cloned and functionally analyzed for further understanding of the molecular mechanism of xylan synthesis in Phyllostachys edulis.[Method] According to the sequence information from the bamboo genome database,primers were designed. A homologous gene from P.edulis playing critical role in xylan synthesis in Arabidopsis thaliana was cloned and named as PeIRX10. The nucleotide sequence and encoded amino acid sequence of PeIRX10 were analyzed using bioinformatics methods. The expression pattern of PeIRX10 in different organs (root, stem, leaf, shoot and flower) was detected by qRT-PCR. The binary vector of PeIRX10 overexpression was constructed using Gateway technology and then transformed into the A.thaliana irx10l(-/-) irx10(+/-) mutant plants by floral dip. Transgenic plants with double mutant background were selected by BASTA and PCR identification. Finally, the conserved function of PeIRX10 in thexylan synthesis was done by phenotype observation, stem section, sugar composition analysis and xylan immunolocalization. [Result] An ORF sequence of PeIRX10(PH01004923G0080,http://www.bamboogdb.org/)from P. edulis was cloned with a length of 1 251 bp,encoding 416 amino acids, with a predicted molecular weiqht of about 46 821 Da and pI of about 6.26. The protein sequence encoded by PeIRX10 exhibited relatively high similarity to those of other plants (>80%), indicating that it belongs to GT47 family. qRT-PCR showed that PeIRX10 was highly expressed in shoots and culms of P. edulis. After overexpression of PeIRX10, complementation of irx10l(-/-)irx10(-/-) double mutant can restore the phenotype with evidence of normal plant height, stem diameter, leaf numbers and sizes. Moreover, the analysis of monosaccharides indicated that the content of xylose and other monosaccharides, such as arabinose, fucose, galactose and glucose in the complementation plants was not significantly different to those of the wild type. Immunostaining of cross sections detected by LM10 labeling revealed strong signals in xylem cells in the irx10l(-/-)irx10(-/-) double mutant when overexpressedby PeIRX10. [Conclusion] Overexpression of PeIRX10 in the irx10l(-/-)irx10(-/-) double mutant rescued the plant growth phenotype and restored the secondary wall thickness, which suggested that the PeIRX10 gene have a function similar to the IRX10 geneof A. thaliana and play an important role in the synthesis of xylan.
Distribution Patterns of Ant Species (Hymenoptera: Formicidae) in Galongla Mountains and Medog Valley of Southeastern Tibet
Liu Xia, Xu Zhenghui, Yu Nana, Zhang Chenglin
2016, 52(11):  88-95.  doi:10.11707/j.1001-7488.20161111
Abstract ( 287 )   HTML   PDF (1027KB) ( 609 )  
References | Related Articles | Metrics
[Objective] In order to reveal the ant distribution feature in southeastern Tibet, the ant distribution patterns in Galongla Mountains and Medog Valley were investigated. This study would provide a reference for biodiversity protection in the region. [Method] Sample-plot and searching methods were used to investigate distribution patterns of ant species in Galongla Mountains and Medog Valley. A sample-plot was set at every 250 m along the altitude. Within each selected sample-plot, five quadrats, each with 1 m×1 m, were set and the quadrat spacing was 10 m. In each quadrat, the ants from the land surface, soil and plants were investigated. After the sample-plot investigation, all places in the sample-plot were investigated by 5 people for one hour. [Result] A total of 20 371 ant individuals were collected from Galongla Mountains and Medog Valley, and belonged to 96 species, 45 genera, and 8 subfamilies of Formicidae. The subfamily of Myrmicinae was richest in genera and species (18 genera and 44 species involved) among the 8 subfamilies, followed by Ponerinae with 24 species in 10 genera. The subfamilies of Pseudomyrmecinae, Dorylinae and Leptanillinae each had only 1 genus and 1 species. The genus of Pheidole was richest in species (10 species involved) among the 45 genera. Most ant species from Galongla Mountains and Medog Valley distributed below the elevation of 2 000 m. Over the all species, Pheidole sagei had the widest range in vertical distribution, and the elevation difference was up to 1 510 m. Valley rainforest was richest in ant species among the 10 habitat types. The ant species nesting and forage on the ground were the richest among all types of nesting and forge sites. In addition, some species could also forge on the tree. Moreover, 95 ant species distributed in south slope and only 5 species distributed in north slope. Myrmica bactriana occupied the widest range of habitats, nesting and forage sites.[Conclusion] The distribution of ant species in Galongla Mountains and Medog Vally is restricted by altitude and plant richness. At the same time, distribution patterns of ant species are affected by slope aspect. As for nesting and forging sites choice, ants firstly ensure the nest relatively dry and the plentiful source of food. Thus ants choose different habitat and foraging and nesting sites, which would avoid the intraspecific and interspecific competition and be in favor of the establishment and development of ant colony.
Maturation Feeding Preference of Adult Agrilus mali and Related Host Plant Leaf Volatiles
Cui Xiaoning, Yi Zhihao, Wang Ming, Liu Deguang, Liao Shujiang, Xu Zheng
2016, 52(11):  96-106.  doi:10.11707/j.1001-7488.20161112
Abstract ( 257 )   HTML   PDF (1606KB) ( 412 )  
References | Related Articles | Metrics
[Objective] The apple buprestid beetle, Agrilus mali (Coleoptera: Buprestidae), is one of the most serious trunk borers in economic forests and has caused destructive damage for Malus sieversii in Xinjiang wild apple forests in recent years. The new preventive and control measures are urgently needed to develop. The objective of this study was to clarify suitable host plants for its adult supplemental feeding and analyze plant leaf volatiles involved in the process.[Method] In this study, four plant species (i.e., Malus halliana, Malus domestica, Pyrus betulifolia and Amygdalus persica) were used to determine its feeding preference and we also analyzed the composition of leaf volatiles from the four species with dynamic headspace collecting procedures and a gas chromatography-mass spectrometer (GC-MS).[Result] The results showed that there were significant differences in host selection and feeding amount of A. mail adult on the above mentioned four species. Adults of A. mali preferred to feed on leaves of M. halliana over M. domestica, and the two species in the genus of Malus were preferred compared to P. betulifolia or A. persica. A. mali adults consumed more leavesof M. halliana than those of M. domestica. It was not found that A. mali adults consumed P. betulifolia or A. persica leaves. In addition, the results from GC-MS analyses indicated that there were significant differences in leaf volatile composition and contents among the four species. A total of 71 volatile components were identified, including five alkanes, two ethers, eight alcohols, two ketones, nine aldehydes, 22 esters, 3 alkenes and 20 terpenoids. Factor analysis showed that factor 1, 2 and 3 explained 41.98%, 31.82% and 19.83% of the total variance, respectively; M. domestica, A. persica and M. halliana contributed the most to the first three factors, respectively.[Conclusion] Both M. halliana and M. domestica were suitable hosts for A. mali adults supplemental feeding, whereas P. betulifolia or A. persica was not suitable. The unique volatile components from the four species, such as (Z)-3-hexen-1-ol, hexyl acetate, (E)-β-ocimene, (E, E)-α-farnesene, (E)-nerolidol, β-caryophyllene, (Z)-3-hexenyl hexanoate and linalool, might play important roles in the process of A. mali adults orientation of host plants, however their specific roles need to be further explored through electroantennogram and olfactometer behavior assays, as well as field studies.
Allelochemical Effects on Aggregation Behaviors of Ips subelongatus(Coleoptera: Scolytidae)
Li Yejing, Kong Xiangbo, Zhang Sufang, Wang Hongbin, Zhang Zhen, Li Chengxi, Jiao Xiangjie, Huo Tian
2016, 52(11):  107-114.  doi:10.11707/j.1001-7488.20161113
Abstract ( 239 )   HTML   PDF (1477KB) ( 509 )  
References | Related Articles | Metrics
[Objective] The Asian larch bark beetle Ips subelongatus (Coleoptera: Scolytidae) is the main larchpest insect in northeastern China. Its continuous outbreaks in these regions pose a serious threat to the larch forests. In order to explore the mutual action mechanism of different allelochemicals from host, non-host, and sibling species as well as to provide a strong technical support in the biological control of I. subelongatus in large area, we investigated the effects of allelochemicals on aggregation behaviors of I. subelongatus.[Method] The behavioral bioassay methods were used to investigate the behavioral differences of I. subelongatus in responses to different enantiomers of its aggregation pheromone components, host volatiles, non-host volatiles, and pheromone components from sibling species.[Result] The aggregation pheromone components were determined to be S-(-)-ipsenol and S-(+)-ipsdienol enantiomeric compositions in field trapping bioassays, which presented very high aggregation activity. Electroantennogram dose-responses studies indicated that antennal response threshold to S-(-)-ipsenol and S-(+)-ipsdienol were all 0.1 μg and the saturation level were 100 μg. Furthermore, the behavioral effects of I. subelongatus to electroantennographic detection (EAD) active semiochemicals from hosts, non-hosts, and closely related bark beetle species were tested in the field trapping bioassays. The attraction of I. subelongatus to a synthetic pheromone source was significantly increased (1.45-1.65 times) when the EAD-active monoterpenes, S-(-)-β-pinene or 3-carene was added to the source. But, addition of EAD-active components S-α-pinene or Terpinolene to trap with synthetic pheromone did not significantly increase trap catches. In field trapping experiments, the non-host volatile (Z)-3-hexen-1-ol reduced I. subelongatus trap catches by 71%, showing a strong inhibitory effects on the pheromone source. Linalool demonstrated the same inhibitory effects as (Z)-3-hexen-1-ol. The other non-host volatiles, for example, Myrtenol, (E)-3-hexen-1-ol, (E)-2-hexen-1-ol, 1-hexanol, 3-octanol, and 1-octen-3-ol had no synergistic or antagonistic effects on I. subelongatus attraction when added to the pheromone trap, whereas cis-verbenol (the major constituent of the aggregaton pheromone of Ips typographus) and trans-verbenol inhibited its attraction to the S-(-)-ipsenol and S-(+)-ipsdienol-containing attractive blend. However, adding the verbenone to pheromone-baited traps did not reduce I. subelongatus attraction, which had an inhibitory effect on I. typographus pheormone baited traps.[Conclusion] EAD-active host and non-host volatiles either had synergized or antagonized, or no effect on I. subelongatus response to its aggregation pheromone; the strong inhibitory effects of semiochemicals from sibling species on I. subelongatus response to its aggregaton pheormone were observed. These results laid the foundation for the development of efficient lures and biological control of I. subelongatus in large area.
An Algorithm of Stem Surface Reconstruction Based on Tangent Plane Projection
You Lei, Tang Shouzheng, Song Xinyu
2016, 52(11):  115-123.  doi:10.11707/j.1001-7488.20161114
Abstract ( 237 )   HTML   PDF (1499KB) ( 590 )  
References | Related Articles | Metrics
[Objective] Three dimension(3D) tree modelling and tree parameters extraction from the three dimensional laser scanning data are the two research focuses in recent years. Combining the two contents, the objective of this study is to reconstruct the irregular triangulation surface of the stem that can be used for extracting stem parameters. [Method] The technology of three dimensional surface reconstruction was used in this study. The algorithm of surface reconstruction which based on tangent plane projection in the three dimensional spaces was improved according to the characteristics of stem point cloud. And the improved algorithm was applied to reconstruct the irregular triangulation as the stem surface model. The improvement was as follows: 1)Radius value was chosen as the criterion for choosing nearest neighbor point set to reduce the influence of the scattered distribution of stem points; 2)The closer the distance between points, the greater the impact between points, the tangent normal vector of a point was calculated by the weighted distance method. And the farther neighbor point was deleted when there are the same project points in the tangent plane of the neighbor point set; 3)According to the geometric topological invariance of the project point in the parallel plane, the calculation of tangent plane was simplified; 4)The transforming of planar point set in the three dimensional spaces to planar point set in the two dimensional spaces was simplified by point set rotating. In the improved algorithm, the current point and its' neighbor point set were projected to the tangent plane and a planar point set was generated. The connected relationship in the Delaunay triangulation of the planar point set was mapped into the stem point. Then, the surface model of the current point and its neighbor points was reconstructed. The global stem surface was obtained through the above reconstruction procedures one point by one point. [Result] The reconstruction experiment of Populus sp. stem showed that the improved algorithm had a better stem model than that of the classic algorithm. The surface reconstruction experiment of stems from different species with different bark properties showed that the reconstructed surface model could clearly show the color information labeled in the field scanning, and the local triangular patch could reveal the scraggly feature of stem surface, and the local triangular patch could show the orientation of the surface triangles. The experiment of extracting stem diameter from the reconstructed surface model showed that, compared with the measured stem diameter in field working, the RMSE value of stem diameter extracted from the reconstructed surface was 0.18 cm, and the accuracy of the reconstructed stem surface was demonstrated.[Conclusion] From the perspective of parameter extraction and using the technology of surface reconstruction, the surface reconstruction algorithm which based on tangent plane projection was improved to reconstructed the stem surface. The reconstructed stem surface had a better visualization effect; it can reflect the real surface features of the tree stem. The improved algorithm can be used for exhibiting the realistic morphological and structural features and constructing realistic three dimensional visualization model of the stem surface. The precise stem reconstructed surface model by the improved algorithm can provide the basic data for stem parameter extraction.
Correlation and Path Analysis of the Components of Fiber Content for Populus×euramericana
Chen Cun, Ding Changjun, Su Xiaohua, Huang Qinjun
2016, 52(11):  124-133.  doi:10.11707/j.1001-7488.20161115
Abstract ( 219 )   HTML   PDF (2076KB) ( 382 )  
References | Related Articles | Metrics
[Objective] The components and their contribution rate of fiber content of the mini-rotation forestry in poplar were analyzed to lay a solid foundation for its breeding work. [Method] Five clones of Populus×euramericana(‘Guariento’, ‘Lingfeng 1’, ‘Lingfeng 3’, ‘Lingfeng 4’and ‘Lingfeng 5’) were selected from plantation at the age of 3 years, the growth traits, wood property and their correlation were analyzed. The relational model was built based on the single plant fiber content with other traits, and the partial correlation analysis and path analysis were carried out between major factors of the fiber content. In this study, analysis of variance(ANOVA), standard deviation analysis and multiple comparison by LSD were applied in all data of each trait, meanwhile the variation coefficient and repeatability were calculated for five clones. Correlation analysis was carried out among every traits, regression analysis was used to analyze and establish the relational model between the single plant fiber content with growth traits and wood property. The partial correlation analysis and path analysis were applied among major factors of fiber content and the contribution rate of major factors was measured. All analysis were based on the software of Excel and SPSS 17.0.[Result] All growth traits (including height, diameter and individual volume) exhibited a relatively wider variation margin and significant differences among the five clones, and they also had a larger genetic proportion in five clones. As for wood property, except for the single plant fiber content, other traits (including lignin, holocellulose, cellulose, basic density, microfibril angle, fiber average length, fiber average width, fiber length-width ratio and fiber coarseness) had a relatively stable expression. The analysis of growth traits showed significant correlation, however, the growth characters and wood properties had an independent correlation. The relational model indicated that individual volume, cellulose and basic density were significantly correlated with the single plant fiber content. The single plant fiber content was largely affected by individual volume, cellulose and basic density, meanwhile the three factors were interacted with each other, these were consistent with the results obtained from the relational model. The path analysis showed that improving individual volume was the best way to improve single plant fiber content, increasing wood basic density was the second, the effect by improving cellulose was the least. The contribution rate of the three major factors was 72.61%, 13.55% and 13.84%, respectively, which is consistent with the results of partial correlation analysis.[Conclusion] All results showed that the main factors of fiber content in Populus× euramericana included individual volume, cellulose and basic density. At the same time, the results indicated that all growth traits (height, diameter and individual volume) had much room for improvement, and these traits were independent with wood properties. Therefore the growth traits should be taken into account as selection criteria.
Construction of Drug-Loaded Polyacrylate/Polyethylene Glycol Semi-Interpenetrating Network Structure in Wood and Its Performances
Liu Binbin, Sun Fangli, Wu Huaping, Yang Xiushu, Rao Jin, Nayebare Kakwara Prosper, Xu Junting
2016, 52(11):  134-141.  doi:10.11707/j.1001-7488.20161116
Abstract ( 405 )   HTML   PDF (2243KB) ( 438 )  
References | Related Articles | Metrics
[Objective] Decay and cracks are the main factors which shorten the service life of oudoor wood. In this study, drug-loaded semi-interpenetrating network and in situ constructed in wood were investigated, in order to improve the decay resistance as well as the dimensional stability.[Method] Polyacrylate/polyethylene glycol(PAA/PEG) polymer alloy with semi-interpenetrating network (SIPN) structure was prepared by one-step polymerization. The relationship between the amount of crosslinker and polymer performance was then studied. The prepared SIPN polymer alloy was characterized by infrared spectroscopy(IR), scanning electron microscope(SEM) and transmission electron microscope(TEM).Populus tomentosa wood was impregnated with 0.5% propiconazole nanoemulsion, acrylic acid, PEG, initiator and crosslinker under vacuum, then in situ polymerized. The dimensional stability and decay resistance properties of wood were also measured. [Result] FTIR results showed that PAA and PEG formed strong H-bond, and the crosslinker might chemically connected them. SEM and TEM indicated that PEG was uniformly dispersed in PAA to form a phase-separate structure. An interpenetrated interface could be observed. Additionally, the dimensional stability tests of wood via three wet-dry cycles showed that the treated wood exhibited minor shrinkage and swelling. The decay resistances of drug-loaded SIPN were greatly improved. [Conclusion] Though the dimensional stability did not meet the expected results, this paper provided a new way to simultaneously improve the dimensional stability and fungi resistances.
Volume Calculation Method and Benefit Analysis of Small-Diameter Wood Sided Sliced Veneer
Ma Yan, Xu Honggang, Yang Chunmei, Xu Shixiang
2016, 52(11):  142-147.  doi:10.11707/j.1001-7488.20161117
Abstract ( 190 )   HTML   PDF (1196KB) ( 378 )  
References | Related Articles | Metrics
[Objective] This paper proposed three measures: slicing double-sided, cutting feed optimization and reasonable selection, in order to solve the inefficiency, low-productivity, more deficiency and other problems of traditional methods for producing veneer.[Method] Firstly, this study proposed to improve volume ratio and production efficiency by the use of sided-slicing processing for small-diameter wood (sides-slicing refers to the continuous longitudinal chipping on both sides of small-diameter wood, it would not stop until 20 mm thickness was remained for a fixed core slice; then slicing the fixed core that remained after multiple processing). Secondly,we gave detailed formulas for both short and long trail direction of sliced veneer volume by conducting mathematical modeling and practical calculating on the ideal small-diameter wood, by which the theoretical basis for reasonable cutting feed was founded. Finally, this paper conducted mathematical modeling on the cross-section of small-diameter wood, and gave the corresponding volume ratio formulas,the actual parameters of the small-diameter wood were measured, and the volume ratio was calculated according to the formula.[Result] By the actual analysis of the small-diameter wood's volume ratio, the conclusion came that the increase of the size of small-diameter wood (The object is ideal small-diameter that has no knots, no radial bending), the sliced veneer volume was gradually increased, the larger size small-diameter wood should be chosen when slicing veneer, so that the volume ratio of small-diameter wood could be improved to a certain degree.[Conclusion] When the veneer is prepared, the sides slicing technology can not only save time, but also reduce manpower, as a result,greatly improve the processing efficiency and volume ratio. On the basis of mathematical modeling for small-diameter wood, sliced veneer volume formula in short and long trail direction and the volume ratio formula, the waste of timber resources could be decreased and the economic efficiency would be improved by reasonable selecting, cutting feed and slicing the wood.
Farmers' Demands on Forestry Socialized Services and its Influencing Factors—A Survey Based on 1 413 Households in 8 Provinces
Liao Wenmei, Zhang Guanglai, Kong Fanbin
2016, 52(11):  148-156.  doi:10.11707/j.1001-7488.20161118
Abstract ( 189 )   HTML   PDF (1218KB) ( 387 )  
References | Related Articles | Metrics
[Objective] This paper tried to clear the demand features of farmers in forestry socialized services (FSS) and its affecting mechanisms of the key factors. The results provided the scientific basis for improving FSS system in China and supporting policy after the reform of collective forestry property.[Method] Taking 1 413 households in 8 Provinces (districts) of China as samples, farmers' demands of FSS and its influencing factors were analyzed by a Logistic model. [Result] In total,the demands of farmers FSS were urgent at the level of 75.26%. In regards of the factors affecting farmer demands on FSS, there were 4 incurring factors that significantly affected 6 kinds' demands of FSS. Those factors were whether to join the forestry professional cooperative organization or not, whether to accept service charges or not, whether to suffer the corresponding difficulties or not and whether to supply corresponding services at local place or not.[Conclusion] In all FSS, farmers' demands prefer to occur on both production and marketing processes. It could significantly stimulate farmers' demands of FSS by improving the effective supply of FSS. The function role of forest cooperatives in FSS was very limited. Service charges had obviously inhibition effects on farmers' demands of FSS. Based on above conclusions, some policy implications were obtained.
Control Potential of the Predator Scymnus (Pullus) posticalis (Coleoptera: Coccinellidae) against Pseudoregma bambusicola (Homoptera: Hormaphididae)
Huang Cuiqin
2016, 52(11):  157-164.  doi:10.11707/j.1001-7488.20161119
Abstract ( 259 )   HTML   PDF (1489KB) ( 345 )  
References | Related Articles | Metrics
[Objective] In order to understand the potential control capacity of Scymnus (Pullus) posticalis against Pseudoregma bambusicola, the optimum predator density, the maximum predatory amount, interference coefficient, instantaneous attack rate and treating time of S. (P.) posticalis fed on Pseudoregma bambusicola were studied. By measuring the life table of S. (P.) posticalis, I investigated the population growth trend of S. (P.) posticalis after feeding with P. bambusicola, to provide a scientific basis for utilizing S. (P.) posticalis to control P. bambusicola. [Method] Under laboratory conditions (temperature (23±1)℃, relative humidity (70%-73%), feeding with fixed time and fixed quantity in plastic cases was conducted to observe and measure the predation of S. (P.) posticalis on P. bambusicola, and to test S. (P.) posticalis's interference effect of its own density. With the application of Data Processing System, I used methods of insect ecology, such as the model of Holling-Ⅱ, the new model of Holling-Ⅲ functional response developed by Wang Shize, and statistic model of functional response of predator to prey developed by Jin Kaizheng, for matching. Furthermore, I analyzed parameters of the optimum predator density, the maximum predatory amount, inference factor, instantaneous attack rate and treating time of P. bambusicola being preyed by S. (P.) posticalis, for studying the regulating effect of S. (P.) posticalis on P. bambusicola. I also used the rearing method of setting veil screen in the forest to measure the life table of experimented S. (P.) posticalis, in order to study S. (P.) posticalis's quantitative characteristic of inner increase capacity. [Result] Under the controlled conditions, the predatory function responses showed that the average predation was able to amount 75 max per day for the 3rd instar S. (P.) posticalis on P. bambusicola, and 81 max per day for adult S. (P.) posticalis, both the 3rd instar and adult S. (P.) posticalis were strong predators of P. bambusicola. The interference effect test on S. (P.) posticalis's density showed that the pest control capacity of S. (P.) posticalis could reach the maximum when the release ratio was 1∶38.8; and the parametric measure result of life table showed that the survival rate of indoor rearing female imagoes was able to reach 63%, and after a generation, the population increased by 85.3638 times. Meanwhile, within the test temperature range, when the reproductive rate (λ) was larger than 1, the population of lab reared S. (P.) posticalis increased in a geometric ratio. By comparing the parameters on life tables, and analyzing the models of functional response, it was shown that S. (P.) posticalis was a strong predator of P. bambusicola, and it had strong reproductive ability, and S. (P.) posticalis had strong pest control capacity against the population of P. bambusicola. [Conclusion] This study indicated that the population of S. (P.) posticalis fed with P. bambusicola grew exponentially, and it had a great potential of preventing and controlling P. bambusicola.
Identification of Pathogens of the Dalbergia tonkinensis Rust Disease
Wang Jiao, Zhou Guoying, Su Shengsong, He Yuanhao, Dong Wentong, Zhang Qian, Liu Jun'ang
2016, 52(11):  165-169.  doi:10.11707/j.1001-7488.20161120
Abstract ( 274 )   HTML   PDF (1261KB) ( 441 )  
References | Related Articles | Metrics

[Objective] A newly found rust disease widely infests leaves of Dalbergia tonkinensis (Papilionaceae) which is a high economic tree species for furniture in China, growing in Chengmai County, Hainan Province, but its pathogen is unknown. This study described the symptoms of the rust disease on D. tonkinensis and its spore morphology, then extracted the pathogen DNA, and amplified it by ITS, with which the pathogen was identified. [Method] The period of occurrence and the rate of disease incidence of D. tonkinensis were investigated on the spot, and symptoms of the disease were observed and recorded. In order to acquire the isolation of pathogen and purification, pathogenicity test was conducted by artificially inoculating healthy leaves of D. tonkinensis with urediniospore suspension. We obtained a strain of D. tonkinensis rust pure culture HNCM1 by single spore separation. The pathogen was identified with the method of the spore morphology observation combined with ITS sequence analysis.[Result] The rust of D. tonkinensis occurred on the leaves and twigs, mainly on young tender leaves, and outbroke 2 times a year, in early April to early June and mid September to early November, respectively. The disease incidence rate of D. tonkinensis was able to reach more than 75%. Uredinia hypophyllous was yellow, and powdery,at early stage, generally bore on the round or oval small projection of the abaxial leaf surface, 0.1-0.5 mm in diameter, scattered or gregarious, which might cause leaf roll or deformation, even death of whole leaf. Urediniospores were globoid or obovoid, (13-21) μm×(10-16) μm, yellow, echinulate, germ pores obscure. Telia were similar to uredini, pale white, colloid, found occasionally when it became cold. Teliospores were oblong-obovoid, oblong-ellipsoid or clavoid, (19-28) μm× (13-16)μm, single cell, wall ca. 1 μm thick, colourless, smooth, pedicels 7-10 μm long. ITS sequence of the pathogen was around 750 bp nucleotide long, and the product was then 640 bp after bidirectional sequencing and splicing, which only shared at most 90% homology with the recorded sequences in Gen Bank. [Conclusion] Based on morphological and molecular identification, the pathogen causing rust disease of D. tonkinensis is Maravalia pterocarpi in Chengmai County, Hainan Province, a fungal species which belongs to pucciniaceae maravalia of basidiomycota. The GenBank accession number of HNCM1 is KU301795. This is the first report of Maravalia pterocarpi paratisizing on D. tonkinensis and causing rust disease in China, as well as the first time to analyze Maravalia pterocarpi from the angle of the molecular biology.

A New Variety of Cunninghamia lanceolata ‘E Sha 1’
Xu Yezhou, Du Chaoqun, Xu Xiangyang, Hu Xingyi, Zhang Yadong, Xu Xiuhuan, Huang Guowei, Fang Lianqun
2016, 52(11):  170-170.  doi:10.11707/j.1001-7488.20161121
Abstract ( 224 )   HTML   PDF (1126KB) ( 433 )  
Related Articles | Metrics
The new variety of ‘E Sha 1’ was selected from Cunninghamia lanceolata cv. Luotian which is a natural variation of C. lanceolata. The new variety grows well with straight trunk, strong adaptability, and strong tolerance to barren and wet. The main morphylogical characteristics are thinner and narrower crowns, shorter branches and the naturally drooping and deciduous old shoots compared with ordinary. The new variety can be propagated by root suckers, grafting or cutting, and can be widely cultivated in the areas in which ordinary C. lanceolata adapts.
An Improved Variety for Samara and Medicinal Use: Eucommia ulmoides ‘Huazhong 10’
Wang Lu, Wuyun Tana, Du Lanying, Liu Panfeng, Du Hongyan
2016, 52(11):  171-171.  doi:10.11707/j.1001-7488.20161122
Abstract ( 203 )   HTML   PDF (1131KB) ( 341 )  
Related Articles | Metrics
Eucommia ulmoides‘Huazhong 10’ was selected through superior tree selection, plantation test and regionalization trail. As a female tree, it had following traits: early fructification with high and stable yields, high content of linolenic acid and strong adaptability. It is suitable for establishing high-yield orchard for both utilization of fruit and bark.
An Elite Variety of Large Berry and Thornless Hippophae rhamnoides‘Chuyi’
Cui Lingjun, Duan Aiguo, Zhang Jianguo, Luo Hongmei, Shan Jinyou, Li Jianxiong, He Caiyun
2016, 52(11):  172-172.  doi:10.11707/j.1001-7488.20161123
Abstract ( 252 )   HTML   PDF (1127KB) ( 420 )  
Related Articles | Metrics
The cultivar of ‘Chuyi’, introduced from Russia, belongs to Hippophae rhamnoides subsp. mongolica. The regional trials showed that ‘Chuyi’ was adaptable to develop at the high latitude regions of China. The cultivar has no or few thorns, its average fruit yield per a plant is above 3 kg at full fruit stage, and its fruit yield per unit area can arrive at 6 000 kg·hm-2.