Welcome to visit Scientia Silvae Sinicae,Today is

Scientia Silvae Sinicae ›› 2017, Vol. 53 ›› Issue (9): 90-96.doi: 10.11707/j.1001-7488.20170911

Previous Articles     Next Articles

The Exploration of Identification Method of Wild Agarwood

Chen Yuan1,2, Shang Lili2, Yang Jinling3, Li Gaiyun1,2, Yin Yafang2, Dai Haofu3   

  1. 1. Research Institute of Forestry New Technology, CAF Beijing 100091;
    2. Research Institute of Wood Industry, CAF Beijing 100091;
    3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101
  • Received:2016-04-14 Revised:2016-05-31 Online:2017-09-25 Published:2017-10-24

Abstract: [Objective] This paper focused on the establishment of effective, accurate and scientific wild agarwood identification technology, and will help to offer a reference basis for the quality evaluation of agarwood standard.[Method] Using alcohol soluble extract content, color reaction, thin layer chromatography (TLC) analysis methods, and the high performance liquid chromatography (HPLC), 10 batches of wild agarwood are identified.[Result] The alcohol soluble extract content of 10 batches of wild agarwood is significantly higher than 10% regulated by Pharmacopoeia(2015). The color reaction of cultivated agarwood shows pale cherry red or cherry red, while the wild ones also show violet,light red and purple except cherry red. The color reaction can assist in the identification of wild agarwood samples.TLC is a simple and sample-saving method. The wild agarwood in TLC show clear fluorescent spots and good separation effect. The most of the wild samples can be identified by TLC. By comparing with similarity evaluation, peak area of 10 batches of wild agarwood, HPLC fingerprints and 9 common characteristic peaks are established. Moreover, the differences of wild and cultivated agarwood HPLC fingerprints, which can identify the wild agarwood effectively and accurately,are determined.[Conclusion] Based on the advantages of each method, this paper proposes HPLC common characteristic peaks with microscopic identification, color reaction, the alcohol soluble extract content and thin layer chromatography to identify the wild agarwood. The results will provide the reference for the establishment of quality evaluation system of agarwood.

Key words: wild agarwood, identification method, high performance liquid chromatography, fingerprint, common characteristic peak

CLC Number: