Scientia Silvae Sinicae ›› 2001, Vol. 37 ›› Issue (2): 51-55.doi: 10.11707/j.1001-7488.20010207
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Weng Yaofu,Chen Yuan,Zhao Yongchun,Zheng Kangle,Huang Shaofu,Zhang Jianzhong
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Abstract:
RAPD——based fingerprinting was carried out for 8 varieties and clones of Castanea mollissima which had been approved by the Zhejiang Inspection Committee of Superior Tree Species.Each of the varieties (clones) was identified by fingerprints.Nine RAPD markers were obtained from repeated screening of 684 random oligonucleotide primers and DNA fingerprints were made for all the used varieties (clones).Stable amplification was obtained through modification of experimental conditions and a complete technical system was established for fingerprinting of Castanea mollissima.Preliminary results for verification of commericial seedilings showed that these fingerprints were reliable.
Key words: Castanea mollissima, Variety (clone) identification, Random amplified polymorphismic DNA, DNA fingerprinting
Weng Yaofu;Chen Yuan;Zhao Yongchun;Zheng Kangle;Huang Shaofu;Zhang Jianzhong. DNA FINGERPRINTING OF CASTANEA MOLLISSIMA ELITE VARIETY (CLONE) SEEDLINGS[J]. Scientia Silvae Sinicae, 2001, 37(2): 51-55.
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URL: http://www.linyekexue.net/EN/10.11707/j.1001-7488.20010207
http://www.linyekexue.net/EN/Y2001/V37/I2/51
