Abstract:

The genetic diversity of chestnut was studied by RAPD molecular markers. The RAPD standardized procedure and the fingerprints database of chestnut were established, which was the principal basement of chestnut breeding. The precise and reliable method identified the chestnut variety was supplied.　With the 16 primers, 119 bands were produced. The 69 bands out of the 119 were polymorphic(58%). The fingerprints database of 46 varieties was established and the optimal combination of primers for these varieties identification was summarized. It was shown that the four primers, out of the 16 primers which can produced the polymorphic bands, could be used to distinguish the 46 varieties tested effectively. The optimal combimation of polymorphic loci was detected for variety discrimination. In order to summarize the better combination of primers, the genetic distance matrix was calculated so as to indicate the particularity of primers combination.

Key words: Chinese chestnut, Variety identification, RAPD molecular markers, Fingerprints