河南省柿种质资源的遗传多样性

Abstract

Abstract: [Objective] The aim of the present study was to elucidate the genetic diversity and relationship among the germplasm accessions of Diospyros kaki in Henan province, meanwhile to identify the germplasms especially those of synonyms and homonyms. [Method] The genetic diversity among 102 germplasms including 93 accessions of D. kaki (18 major cultivars, 55 local varieties and 20 wild persimmons) originating from different areas in Henan and nine materials used as control which belong to five relative species i.e., D. lotus, D. oleifera, D. glaucifolia, D. virginiana and Jinzaoshi (D. spp.), and D. kaki var. sylvestris were analyzed based on the polymorphic SSR (simple sequence repeat) primers selected from those developed for Diospyros in previous reports. [Result] A total of 159 polymorphic DNA fragments i.e., bands, were obtained from the analysis of genetic variation among all 102 accessions by 17 pairs of SSR primers with an average of 9.35 bands per pair of primers ranging from 5 (8125/DC592401) to 14 (ssrDK11/DQ097479 and ssrDK14/DQ097482). The number of band patterns was 508 in total with an average of 29.88 per pair of primers ranging from 8 (5553/DC585710) to 53 (ssrDK11/DQ097479). The total number of unique band patterns was 267 with an average of 15.71 per pair of primers ranging from 2 (5553/DC585710) to 37 (ssrDK11/DQ097479). The rate of unique band patterns ranged from 73.68% to 25.00% and the rate of germplasm identified ranged from 1.96% to 36.27%. The average value of polymorphism information content (PIC) was 0.839 7 ranging from 0.466 7 to 0.964 7 and the average Shannon's information index (I) was 2.658 6 ranging from 1.112 1 to 3.596 7. Observed heterozygosity (H_o) ranged from 0.147 1 to 0.960 8 with an average value of 0.777 4. The highest genetic diversity level was found in three loci ssrDK11/DQ097479, ssrDK14/DQ097482 and mDp17/EF567410 by comprehensive comparison. The result obtained from principal coordinate analysis was roughly the same as that from cluster analysis: There were obvious genetic differences among different species. All germplasm accessions of D. kaki were divided into the same group which was clearly different from the samples of other closely related species. Compared with the other related species, the relationship between D. kaki and D. virginiana was relatively distant. All germplasm accessions tested including some homonymous or synonymous cultivars were identified by the 17 loci. [Conclusion] The results in present study revealed high level of genetic diversity among D. kaki germplasm accessions in Henan province with large variations and high heterozygosity. Moreover, the method was proved to be efficient in germplasm identification of D. kaki.

Key words: Diospyros kaki, genetic diversity, SSR markers, genetic relationship, germplasm identification

CLC Number:

Liang Yuqin, Zhang Jiajia, Liang Jinjun, Li Fangdong, Han Weijuan, Fu Jianmin. Genetic Diversity among Persimmon Germplasms in Henan Province[J]. Scientia Silvae Sinicae, 2015, 51(6): 71-80.

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Genetic Diversity among Persimmon Germplasms in Henan Province

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