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Scientia Silvae Sinicae ›› 1995, Vol. 31 ›› Issue (2): 125-131.

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ANALYSIS OF MASSON PINE NEEDLE VOLATILES AND THEIR ELECTROANTENNOGRAM ACTIVITY WITH MASSON PINE CATERPILLAR

Chenghua Zhao,Deming Wu,Yunhua Yan,Qun Li   

  1. Chinese Academy of Sciences Institute of Zoology Beijing 100080
  • Received:1993-11-19 Online:1995-03-25 Published:1995-03-25

Abstract:

Needle volatiles of Masson pine, Pinus massoniana were obtained by solvent extraction and following vacuum distillation.20 Components were identified from the volatiles with GC and GC-MS.They were mainly monoterpenes, monoterpene alcohols, monoterpene acetates and sesquiterpenes. The role of the needle volatiles in the selection of oviposition site for the masson pine caterpillar, Dendrolimus punctatus was investigated with normal and antennectomized female moths.The experiments showed that normal moths deposited much more eggs on the pine needles than antennectomized ones.Electroantennogram(EAG)study showed that both sexes of unmated and mated moths had strong EAG responses to the needle volatiles.The volatiles were fractionated by preparative GC and GC fractions were assayed for EAG activities to unmated and mated females.EAG activity was found in the fractions containing some of monoterpenes, monoterpene alcohols and monoterpene acetates.The largest EAG response was elicited by the fraction mainly containing β-pinene.

Key words: Pinus massoniana, Needle volatiles, Terpenes, Dendrolimus punctatus, Olfaction, Electroantennogram