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25 March 1995, Volume 31 Issue 2
STUDY ON THE PLANT REGENERATION FROM POPULUS DELTOIDES EXPLANT TRANSFORMED WITH BT. TOXIN GENE
Ying Chen,Yifan Han,Yingchuan Tian,Ling Li,Shaoji Nie
1995, 31(2):  97-102. 
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The effective regenetation system for Populus deltoides was developed firstly in the experiment. Based on the system, Agrobacterium tumefaciens LBA 4404 strains hauboring the Bt. toxin gene expression vectors pB48.214 and pB48.215 respectively were uesd for transformation of poplar plants by means of the leaf disc method. After transformating, adventitious buds were formed at the cut of explant on the media with two concentrations of kanamycin 50mg/l and 30mg/l nearly one month later. Variance analysis showed that the effects of two kanamycin concentrations on the transformation rate and mean adventitious bud numbers were significant at p < 0.05. When shoots reached 1cm long, they were transferred to rooting media and supplemented with 30mg/l and 50mg/l kanamycin and the rooting percentage was 9.62% and 8.02% respectively. PCR analysis showed that Bt. genes were successfully transformed into Populus deltoides chromosomes.

A PRELIMINARY STUDY ON THE NET PHOTOSYNTHESIS OF MASSON'S PINE
Jixing He,Shouqian Zhu,Yuehong Gan
1995, 31(2):  103-109. 
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This paper mainly deals with the changes of the rate of net photosynthesis (Pn) which varied with leaf age, including primordial leaf of seedlings and 1 or 2-year-old needles of young trees of Pinus massoniana. The habitat, productive structure and the variation of net photosynthesis and net primary productivity were also studied of the groves which were formed by natural regeneration. The research results showed that peak value of Pn occurred during July for primordial leaves and the rate of Pn varied in a biparabolic curve for 1 or 2-year-old needles. In order to improve the net primary productivity of the natural stands of this species, the productive structure of the groves should be properly controlled.

TREND SURFACE ANALYSIS ON DBH DATA OF CHINESE FIR PROVENANCES
Bowang Chen,Jusheng Hong
1995, 31(2):  110-115. 
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Data of 43 Chinese fir provenamces from 55 experimental sites was analyzed with the method of trend surface analysis (TSA). The result showed this Fourier trend surface analysis usually had higher precision than that of polynomial, it could clearly drew out best and lower provenance regions, the subject effect on distinguishing provenance could be reduced, and it would be a powerful tool for provenance research.

PRODUCTION AND ROOTING ABILITY OF CUTTINGS COLLECTED FROM LARIX KAEMPEFERI (LAMB.) CARR. ORTETS HEDGED IN DIFFERENT TYPES
Xiaoshan Wang,Changgeng Ma,Jintang Kou,Zhongxiu Huo,Guolin Lu
1995, 31(2):  116-124. 
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One year old outstanding seedlings and seedlings of superior families of L. Kaempferi(LAMB.)CARR.were planted in a hedged orchard in a spacing of 60cm×50cm.Ortets in the experimental area were hedged in three different types:Low-sized table type, ortets were topped at the height of 20cm above ground and all lateral branches remained on the stems were truncated at the length of 4cm-5cm from the base; Middle-and long-sized cylindricality types, ortets were topped at the height of 70cm and 140cm respectively from the ground and branches remained on the stem were treated by the same method mentioned above.Repeated topping was taken to keep the height of ortets under control.Cuttings were collected twice a year.For the first time, 3-4 shoots were cut off at the length of 1cm-1.5cm from the base and the others were cut off at the base in the end of April for preparing woody cuttings. Second collection was done in the late of June when new shoots grew more than 13cm long.3-4 shoots(preferred shoots less than 13cm)were remained on each truncated branch and allowed to continue growing.The other shoots were all cut off at the base for preparing semi-woody cuttings.Cutting production and its rooting ability were studied each year.Influence of truncated length of lateral branches on shoot sprout were also investigated. Growth response of ortets varied with hedging types.Compared with ortets of middle-sized cylindricality type and low-sized table type, thelong-sized cylindricality ortets were the biggest one in size; and produced as many as 284.6 cuttings in average each year when they were 3-year-old which is significantly higher than ortets of low-sized table type(135.5 cuttings)and middle-sized cylindricality type(209.8 cuttings).There were no significant differences in rooting ability among cuttings(both of woody and semi-woody cuttings)taken from ortets hedged in the different types.Rooting to ground of woody cuttings was ranged 70.0-77.8% if cuttings were treated with 0.005% IBA. However rooting ability could be expected even better for semi-woody cuttings(87.7%-90.0%).For ortets with long-sized cylindricality type, rooting percentage of cuttings collected from branches close toground(0-20cm from ground)was significantly higher than that of cuttings collected from the top of ortets(110-140 cm)which showed the positional effect. It was harmful for the occurrence of new shoots to cut off lateral branches at the base, but large quantity of twigs came out on the remained portion of branches as the branches were truncated at the length of 4-5 cm from the base.It was found that optimal period for collecting woody cuttings was the stage of development when the needles on the ortets fully extended in the late spring.

ANALYSIS OF MASSON PINE NEEDLE VOLATILES AND THEIR ELECTROANTENNOGRAM ACTIVITY WITH MASSON PINE CATERPILLAR
Chenghua Zhao,Deming Wu,Yunhua Yan,Qun Li
1995, 31(2):  125-131. 
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Needle volatiles of Masson pine, Pinus massoniana were obtained by solvent extraction and following vacuum distillation.20 Components were identified from the volatiles with GC and GC-MS.They were mainly monoterpenes, monoterpene alcohols, monoterpene acetates and sesquiterpenes. The role of the needle volatiles in the selection of oviposition site for the masson pine caterpillar, Dendrolimus punctatus was investigated with normal and antennectomized female moths.The experiments showed that normal moths deposited much more eggs on the pine needles than antennectomized ones.Electroantennogram(EAG)study showed that both sexes of unmated and mated moths had strong EAG responses to the needle volatiles.The volatiles were fractionated by preparative GC and GC fractions were assayed for EAG activities to unmated and mated females.EAG activity was found in the fractions containing some of monoterpenes, monoterpene alcohols and monoterpene acetates.The largest EAG response was elicited by the fraction mainly containing β-pinene.

A NEW SPECIES OF CARYANDA FROM JINZHONGSHAN AREA OF GUANGXI, CHINA
Guofang Jiang,Zhemin Zheng
1995, 31(2):  132-134. 
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This paper reports a new species of Caryanda Stal from Jinzhongshan area of Guangxi, Type specimens are preserved in the Institute of Zoology, Shaanxi Normal University and Institute of Biology, Guangxi Academy of Sciences.Caryanda jinzhongshancnsis sp.nov.(figs, 1-7).This new species resembles Caryanda vittata Li et Jin, but differs from the latter in the following characters:(1)the width of vertex longer than its length about 2, 2-3, 3 times; (2)the length of interspace of mesosternal lobes longer than the width about 1.3-1.6 times; (3)in ventral, the apex of subgenital plate of male truncate; (4)male anal plate with median carina throughout; (5)cercus of male longawl.

STUDIES ON THE FIRE-RESISTANCE OF 37 SPECIES OF CONIFEROUS AND BROADLEAF TREES AND IT'S APPRAISAL
Cunji Chen,Zongming He,Donghua Chen,Yonglai Huang,Jinsheng Xie
1995, 31(2):  135-143. 
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Through the measure of moisture content, crude fat, crude ash, silica, volatile oil, burning point, combustion heat, burning speed, etc., of the leaf and the use of the method of A.I.D., 37 species of coniferous and broadleaf trees are divided into six grades according to their fire-resistance property.The four species including Trachycarpus fortunei, Michelia macclurei var.Sablbnea, etc., are in the first grade, their fire-resistance property are the best.The nine species belong in the second grade.Pinus massoniana, Platycladus orientalis and Cryptomeria fortunei belong in the sixth grade, their fireresistance property are the worst.The results as above-mentioned finally indicate the fire-resistance regulation of the main coniferous and broadleaf trees in South China and provide the theoretical evidence for the selection of fireprevention tree species.

STUDIES ON PHASSUS ACTINIDIAE YANG ET. WANG INJURIOUS TO ACTINIDIA SPP.
Yuyin Lin,Zhiyuan Wu,Jie Jiang
1995, 31(2):  144-149. 
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Phassus actinidiae has been recently a new stem-boring pest which injuries Actinidia trees severely in the north of Fujian Province.The study on its bionomics and the control measures were carried out in Actinidia Experiment Station in Jianning Fujian during 1984-1989.The results show that the insects reproducd one generation a year and overwinter as eggs on ground near the tree stumps.The larvae bore the phloem and pith and form tunnels inside and dust bags outside the injured parts, which hinder the normal transportation of nutrients and water, as a result, it leads to discolor of shouts and leave even dieback of the tree.Apart from selecting plant varieties resistant, some effective control measures must be applied, such as pouring water dilution of 2.5% Decamethrin the ratio of 1:5000(insecticide:water) or stuffing the tosci mud made of 90% Dipterex crystals into the burrows on the trunks of host plant; smearing Vaseline mixed with Dichlorvos around the trunk to prevent the larvae from climbing up; killing the larvae with fine iron wires and capturing the adults immediately after incubation etc.

A STUDY ON TRUCK TAIL SHAFT
Shijie Fang
1995, 31(2):  150-154. 
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The moving track is broadened when a truck-trailer goes round curver in a road. In general, the increment of the track of a shaft style truck-trailer is less than that of a seperating style one. The track increment of a truck-trailer equipped with tail shaft turning compensator is just half of that of a seperation style truck—trailer. So, it is an effective method to set tail shaft to reduce the track increment. The length of a tail shaft for a truck-trailer can vary according to horizontal and vertical arc conditions and the tail structure of the truck-trailer, and the tail shaft is designed as a multijoint device which can be pulled forth and back.

A STUDY ON TYLOSES AND GUMS IN THE VESSELS OF CHINESE HARDWOOD
Xiaomei Jiang,Lifei Zhang,Yin Zhou
1995, 31(2):  155-159. 
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The occurrence and morphology of tyloses and gums in vessels of 102 Chinese hardwood were investigated using SEM. Tyloses were present in 49 species and gums in 12 species. Morphological types of tyloses are: foamed, ladder-, collasped-shaped, globular. Morphological types of gums are: vessels occlusied, thin layer, small droplet. The mean values of length and width pit aperture of vessel to parenchyma pit were greater than 3 and 2μm, respectively, in species having tyloses and were less than these values in species having gums. The obeservation of ultrathin section of Robinia pseudoacacia wood by TEM showed: the tyloses walls has a compound middle lamella and primary walls with simple pits. There are more tyloses and gums in vessele of pith of 3 species. The content of tyloses and gums decreased in vessels of each growth ring from pith to bark.

THE EFFECT OF TEMPERATURE ON DORMANCY RELEASE IN BUDS OF TEMPERATE WOODY PERENNIALS: A REVIEW
Zeping Jiang
1995, 31(2):  160-168. 
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This paper is a review of the literature on the effect of temperature on dormancy release in buds of woody perennials. The following topics are reviewed: a) Definition and interpretation of dormancy; b) Dormancy terminology; c) Base temperature or critical temperature in woody plants; d) Effect of temperature - chilling, moderate temperature, high temperature, fluctuating temperature, and the duration of treatment - on dormancy release in woody plants. Finally, the problems that have arisen from current studies on dormancy release are discussed.

ADVANCES IN RESEARCH OF GENETIC PARAMETER ESTIMATIONS FOR CLONAL BREEDING OF FOREST TREES
Qi Wang,Zhiti Zhu
1995, 31(2):  169-176. 
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Genetic parameter estimation is one of the most important steps in tree breeding. Tree clonal breeding has many advantages, of which are mainly simple in breeding method, short breeding circle and high gain. But it larcks a good set of methods in genetic parameter estimation. At present, the estimating genetic parameters in clonal breeding of forest trees nearly all adopt the methods used in sexual breeding, which is likely to affect precise estimation of genetic parameters. This paper presents a precise fomula of genetic parameter estimation for clonal breeding after an investigation on clonal materials. A discussion was also made on the number of clones used in the establishment of plantations.

A SURVEY OF STUDY ON THE REDUCE VIBRATION OF THE HIGH-HANDLED CHAIN SAWS
Souqian Chen,Ping Wang,Guoyu Song
1995, 31(2):  177-180. 
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A chain saw is the power tool widly used in forestry producer. It acts on the wood producation. However, from ergonomists angle the in body and mind of the operator are influenced diredly by severe vibration of the chain saw, through look at matter. The paper reviewes that the Chinese high-handled is harmful to the body by vibration. In the paper, courses to reduce vibration and acquired achievement in research were discussed to the chinese high-handled chain saws for the last ten years and more, and it pointed out the research direction of the reduce vibration.

STUDIES ON THE CLONES OF GENE-TRANSFORMED POPULUS TOMENTOSA
Junbao Zheng,Feng Wang,Qunlu Liu,Xuexian Pu,Weilun Chen
1995, 31(2):  181-184. 
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Gene R1000 and R 1000(PTVK85)were transformed to Populus tomentosa, and their clones were achieved. After the vitro plantlets of the clones were transplanted to field, their cutting experiment, growth measurement and morphological observation were carried out. The clones whose rooting rates were improved significantly accounted for 69.2%of the total tested clones. Of these clones, only 44.4% were observed neither to reduce their productivity nor to change their morpgological characters. For the rest of the clones, the height growth of one year-old cutting plants decreased about 30%. The total dry weight of stems, branches and leaves was 50 % lower. There was a larger change in leaf morphological characters. The branch angles was narrow and the growth increment of branch was higher. Therefore, their economic values for timber production were reduced. Besides, the plants of these clones appeared to have more buds and relatively higher net leaf weight.

STUDY ON THE CHEMICALS IN BARK OF POPULUS TOMENTOSA CARR.RESISTANT TO ANOPLOPHORA GLABRIPRNNIS MOTSH.
Rui Wang,Guansheng Ju,Xixiang Qin
1995, 31(2):  185-188. 
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To study the resistance of the chemicals which are contained in the bark of Populus tomentosa Carr. To Anoplophora glabripennis Motsh, the quantity of amino acid, suger, phenolic acid and phenolic gluconside were examined in comparition with P. alba L.. It has been found that some phenolic acid and phenolic gluconside are related to the insect resistance of Populus. Populin is worth being further studied for its insect resistance. Besides, the resistance of white poplar and others were discussed here.

A STUDY OF WOOD ELASTIC PARAMETERS PARALLEL TO GRAIN IN COMPRESSION TEST BY USING RESISTANCE STRAIN GAGES
Meng Gong
1995, 31(2):  189-191. 
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Three elastic parameters of masson pine were measured by using resistance strain gages.The results of the experiments show that(1) EL parallel to grain in compression can be measured by strain gages fixed only on radial surface (LR-plane). (2)There is a good agreement between EL and air-dry density. (3) A good estimate of the compliance can be obtained from the derived equations.