Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (6): 138-145.doi: 10.11707/j.1001-7488.20100622
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Song Jianying
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This study used buds of <i>Eucalyptus dunnii, </i>that survived after the freezing disaster in the northern Fujian Province, as explants and employed micropropagation methods (shoot inducement, proliferation, rooting and plantlet hardening and so on) to breed coldresistant strain. An orthogonal experiment was used to screen the best media for each propagation stage. The results showed that: 1) Bud inducement was initiated on a MS medium containing 1.0 mg·L <sup>-1</sup> KT, 0. 05 mg·L <sup>-1</sup> NAA. The inducement rate of valid buds reached to 76%. 2) The shoots were subcultured and multiplied on an improved MS medium containing 0.8 mg·L <sup>-1</sup> KT, 0.1 mg·L <sup>-1</sup> NAA and 10 mg·L <sup>-1</sup> cysteine. The two media were modified by adding 30 g·L <sup>-1</sup> sucrose and adjusted to pH5.8. 3) Rooting medium was the half strength of MS medium supplied with 1.0~1.5 mg·L <sup>-1</sup> ABT and 0.01 mg·L <sup>-1</sup> IBA, with which the rooting rate was 84% in a two-step rooting method. This medium was modified by adding 20 g·L <sup>-1</sup> sucrose and adjusted to pH5.8. 4) Transplanting medium was a mixture of 20% bark, 30% husk, 40% sawdust, 28% peat moss and 2% composite fertilizers, and was used for transplanting the plantlets. The survival rate of the plantlets was 100% after transplantation, and 18% higher than that in the natural soil. Growth in height of the transplanted seedlings in the medium was 23.9 cm in 4 month and was 9.9 cm higher than that in the natural soil. The result showed that transplant survival rate of <i>E. dunnii</i> plantlets was much improved and seedlings grew more healthily and strongly in the light matrix.
Key words: <, i>, Eucalyptus dunnii, <, /i>, cold strain, induction, subculture, rooting, plantlet regeneration
Song Jianying. Bud Micropropagation and Plantlet-transplanting Techniques for Breeding Cold-resistant strain of <i>Eucalyptus dunnii</i>[J]. Scientia Silvae Sinicae, 2010, 46(6): 138-145.
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URL: http://www.linyekexue.net/EN/10.11707/j.1001-7488.20100622
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