油松成熟胚培养不定芽状态对其生根状况的影响

doi:10.11707/j.1001-7488.20140519

Abstract

Abstract:

The mature embryo of Pinus tabulaeformis was used as explants to induce adventitious buds and root on modified 1/2MS medium. The results showed that the largest multiplication rate with strong buds was obtained when adventitious bud cluster was divided into three pieces. The rooting rate of adventitious buds was 91.7%. The adventitious bud clusters with diameter larger than 0.9 cm, and the shoots with length longer than 6 mm had higher rooting rate. In the process of shoot elongation and multiplication, short culture duration could lead to a higher rooting capacity of the adventitious bud cluster, and the 28-days culture period was optimal for rooting, leading to a rooting rate of 73.3%. Adventitious bud clusters with big biomass were easily to root, and the higher the rooting rate the bigger the bud cluster biomass.

Key words: Pinus tabulaeformis, mature embryo, modified 1/2MS medium, adventitious bud induction, adventitious bud rooting

CLC Number:

S723.1⁺3

Mai Kaile, Shao Liqun, Li Rongzhen, Dong Lifen, Lan Jianhua. Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaeformis on Its Rooting[J]. Scientia Silvae Sinicae, 2014, 50(5): 147-153.

References

常建国,王庆云,武秀娟,等.2013.山西太行山不同林龄油松林的水量平衡.林业科学,49(7):1-9.
(Chang J G, Wang Q Y, Wu X J, et al. 2013. Water balance of different age pinus tabulaeformis forests in Taihang Mountain,Shanxi. Scientia Silvae Sinicae, 49(7):1-9. [in Chinese] )
陈丽培,王国霞,沈永宝.2012.油松种子萌发初始阶段的呼吸代谢.林业科学,48(5):150-153.
(Chen L P, Wang G X, Shen Y B. 2012. Respiratory metabolism of pinus tabulaeformis seeds at the initial germinating stages. Scientia Silvae Sinicae, 48(5):150-153. [in Chinese] )
董丽芬,肖颖,邵崇斌.2006.氮、磷、钾元素形态配比及浓度对油松胚培养的影响.西北林学院学报, 21(3):64-66.
(Dong L F, Xiao Y, Shao C B. 2006. The effect of form and concentration of nitrogen,phosphorus,potassium on inducing embryos of chinese pine. Journal of Northwest Forestry University, 21(3):64-66. [in Chinese] )
冯晓燕,刘宁,郭晋平,等.2013.控制光照条件下华北山地4个混交树种幼苗幼树的形态响应和可塑性. 林业科学,49(11):42-50.
(Feng X Y, Liu N, Guo J P, et al. 2013. Morphological responses and morphological plasticity indices of seedlings and saplings of four woody species in montane forest of northern China to experimental light regimes. Scientia Silvae Sinicae, 49(11):42-50. [in Chinese] )
孔冬梅,万婷.2010.油松成熟胚培养直接器官发生与植株再生.植物研究,30(6):668-673.
(Kong D M, Wan T. 2010. Direct organogenesis and plantlet regeneration from mature zygotic embryos of Chinese pine(Pinus tabulaeformis carr.). Bulletin of Botanical Research, 30(6):668-673. [in Chinese] )
李兵兵,秦琰,刘亚茜,等.2012.燕山山地油松人工林林隙大小对更新的影响.林业科学,48(6):147-151.
(Li B B, Qin Y, Liu Y X, et al. 2012. Effects of gap size on regeneration of Pinus tabulaeformis plantation in the Yanshan Mountain. Scientia Silvae Sinicae, 48(6):147-151. [in Chinese] )
李科友,唐德瑞,李林,等.2008.离体条件下西黄松成熟合子胚不定芽的诱导及植株再生.林业科学,44(5):38-45.
(Li K Y, Tang D R, Li L, et al. 2008. Adventitious bud induction and plantlet regeneration in vitro from mature zygotic embryos of ponderosa pine. Scientia Silvae Sinicae, 44(5):38-45. [in Chinese] )
买凯乐,韩富亮,董丽芬,等.2007.N、P、K元素对油松试管苗生长及生根诱导的影响.西北林学院学报,22(5):69-71.
(Mai K L, Han F L, Dong L F, et al. 2007. The effect of nitrogen phosphorus and potassium elements on growth and root induction of test tube seedlings of Chinese pine. Journal of Northwest Forestry University, 22(5):69-71. [in Chinese] )
钮世辉,袁虎威,陈晓阳,等.2013.油松雌雄球花高通量基因表达谱芯片分析.林业科学,49(9):46-51.
(Niu S H, Yuan H W, Chen X Y, et al. 2013. Microarray analysis of large scale gene expression profiles between male and female cones of Pinus tabulaeformis. Scientia Silvae Sinicae, 49(9):46-51. [in Chinese] )
彭丽萍,董丽芬.2008.樟子松组织培养不定根诱导的研究.西北林学院学报,23(1):100-103.
(Peng L P, Dong L F. 2008. Root regeneration from adventitious buds in Pinus sylvestris var. mongolica. Journal of Northwest Forestry University, 23(1):100-103. [in Chinese] )
齐力旺,杨云龙,韩素英,等.1995.油松封顶芽的组织培养.植物生理学通讯,31(1):40-44.
(Qi L W, Yang Y L, Han S Y, et al. 1995. Tissue culture of dormant buds from Pinus taulaeformis. Plant Physiology Communications, 31(1):40-44. [in Chinese] )
尚峰男,宋少宇,田菊,等.2013.油松离体花粉管的生长及微丝骨架分布.林业科学,49(4):34-38.
(Shang F N, Song S Y, Tian J, et al. 2013. In vitro pollen tube growth and distribution of microfilaments in pollen tube of Pinus tabulaeformis. Scientia Silvae Sinicae, 49(4):34-38. [in Chinese] )
谢斌,郭俊荣,杨培华,等.2007.影响油松种子园产量的因素及提高对策.西北林学院学报,22(6):74-77.
(Xie B, Guo J R, Yang P H, et al. 2007. Factors affecting the seed yield of Pinus tabulaeformis seed orchard and countermeasures. Journal of Northwest Forestry University, 22(6): 74-77. [in Chinese] )
徐化成.1993.油松.北京:中国林业出版社,18-38.
张冬梅,张华新,沈熙环,等.2004.油松种子园交配系统的时空变化研究.林业科学,40(1):70-77.
(Zhang D M, Zhang H X, Shen X H, et al. 2004. Study on temporal and spatial change of the mating system in a seed orchard of Pinus tabulaeformis. Scientia Silvae Sinicae, 40(1):70-77. [in Chinese] )
郑均宝,王进茂,杜克久,等.1996.油松体细胞无性系的建立.遗传学报,23(4):120-132.
(Zheng J B, Wang J M, Du K J, et al. 1996. Establishment of somatic cell clones of Chinese pine. Journal of Genetics and Genomics, 23(4):120-132. [in Chinese] )
朱丽华,吴小芹.2006.湿地松成熟合子胚直接器官发生及植株再生.林业科学,4(28):25-29.
(Zhu L H, Wu X Q. 2006. Plantlet regeneration via organogenesis from mature embryos of Pinus elliottii. Scientia Silvae Sinicae, 4(28):25-29. [in Chinese] )
Alonso P, Moncaleánb P, Fernándeza B, et al. 2006. An improved micropropagation protocol for stone pine (Pinus pinea L.). Annals of Forestry Science, 63(8): 879-885.
lvarez J M, Majada J, Ordás R J. 2009. An improved micropropagation protocol for maritime pine (Pinus pinaster Ait.) isolated cotyledons. Forestry, 82 (2): 175-184.
Becw M R,Nagmani R,Wann S R. 1990.Initiation of embryogenic cultures and somatic embryo development in loblolly pine (Pinus taeda).Can J For Res, 20: 810-817.
Capuana M, Giannini R. 1995. In vitro plantlet regeneration from embryonic explants of Pinus pinea L. In Vitro Cell Dev Biol Plant, 31:202-206.
Carneros E, Celestino C, Klimaszewska K, et al. 2009. Plant regeneration in Stone pine (Pinus pinea L.) by somatic embryogenesis. Plant Cell, Tissue and Organ Culture, 98(2): 165-178.
Cortizo M, de Diego N, Moncaleán P, et al. 2009. Micropropagation of adult Stone pine (Pinus pinea L.). Trees, 23(4): 835-842.
Dumas E, Monteuuis O. 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cell, Tissue and Organ Culture, 40(3): 231-235.
Gonzzalez M V,Rey M,Tavazza R. 1998. In vitro adventitious shoot formation on cotyledons of Pinus pinea.Hort Science, 33(4):749-750.
Kong L, von Aderkas P. 2011. A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer. Plant Cell Tissue Organ Cult, 106(1):115-125.
Le-Feuvre R, Trivio C, Sabja A M. 2013. Organic nitrogen composition of the tissue culture medium influences Agrobacterium tumefaciens growth and the recovery of transformed Pinus radiata embryonal masses after cocultivation. In Vitro Cell Dev Biol-Plant, 49: 30-40.
Moncaleán P, Alonso P, Centeno M L, et al. 2005. Organogenic responses of Pinus pinea cotyledons to hormonal treatments: BA metabolism and cytokinin content. Trees Physiol, 25: 1-9.
Montalbán I A, de Diego N, Moncaleán P. 2010. Bottlenecks in Pinus radiata somatic embryogenesis: improving maturation and germination. Trees, 24(6):1061-1071.
Montalbán I A, De Diego N, Moncaleán P. 2011. Testing novel cytokinins for improved in vitro adventitious shoots formation and subsequent ex vitro performance in Pinus radiata. Forestry, 84(4): 363-373.
Parasharami V A, Poonawala I S, Nadgauda R S. 2003. Bud break and plantlet regeneration in vitro from mature trees of Pinus roxburghii Sarg. Current Science, 84(2):203-208.
Park Y S, Lelu-Walter M A, Harvengt L, et al. 2006. Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster and P. sylvestris at three laboratories in Canada and France. Plant Cell Tissue Organ Cult, 86: 87-101.
Percy R E, Klimmaszewska K, Cyr D R. 2000. Evaluation of somatic embryogenesis for clonal propagation of western white pine. Canadian Journal of Forest Research, 30(12):1867-1876.
Saborio F, Dvorak W S, Donahue J K, et al. 1997. In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite. Tree Physiology, 17:787-796.
Stoji?i?D, Budimir S,?ulafi D? L. 1999. Micropropagation of Pinus heldreichii. Plant Cell Tissue Organ Cult, 59: 147-150.
Tang W, Newton R J. 2005. Plant regeneration from callus cultures derived from mature zygotic embryos in white pine (Pinus strobes L). Plant Cell Reports, 24:1-9.

[1]	Zhu Lihua;Wu Xiaoqin. Plantlet Regeneration via Organogenesis from Mature Embryos of Pinus elliottii [J]. Scientia Silvae Sinicae, 2006, 42(08): 25-29.
[2]	Wu Yanjun;Zhang Shanglong;Xie Ming;Cheng Junwei;Qin Yonghua;Qin Qiaoping. Plantlets Regeneration from Immature Embryos and Cotyledons of Peach [J]. Scientia Silvae Sinicae, 2005, 41(5): 45-50.
[3]	Li Keyou;Tang Derui;Zhu Hailan;Zhao Zhong;Guo Aiguang. Adventitious Bud and Root Formation of Pinus ponderosa Cultured in vitro [J]. Scientia Silvae Sinicae, 2004, 40(4): 63-67.
[4]	Zhang Huijun;Luo Fengxia. STUDY ON IN VITRO CULTURE OF IMMATURE EMBRYO OF FRAXINUS MANDSHURICA [J]. Scientia Silvae Sinicae, 2003, 39(3): 63-69.
[5]	Wu Yuanli;Yan Xuecheng. THE MATURE EMBRYO OF GINKGO BILOBA L: IN VITRO CULTURE AND ITS CYTOHISTOLOGICAL STUDIES [J]. , 1998, 34(4): 8-13.
[6]	Yuan Qiaoping;Dong Maoshan;Christian Jay-Allemand. ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM THE CULTURE OF WALNUT (JUGLANS REGIA L.) [J]. , 1990, 26(6): 495-499.

Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaeformis on Its Rooting

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