马大杂种相思腋芽高效组培增殖体系

doi:10.11707/j.1001-7488.20140607

Abstract

Abstract:

To build an efficient axillary bud multiplication system of Acacia mangium × A. auriculiformis, the aseptic plantlets of A. mangium × A. auriculiformis were used to screen the optimal protocol for in vitro multiplication. The optimal multiplication medium and period, length of the sterile stems, subculture times, rooting and transplant survival rate were studied. The results showed that after being cultured 7 times on improved-MS+ 0.5 mg·L^-16-BA+0.1 mg·L^-1 NAA +0.04 g·L^-1Ac(with combination of 3% sugar and 7 g·L^-1 agar),which was the best multiplication medium screened from the trial test, the average proliferation times of the stem with one axillary bud were 2.52, and the propagation coefficient of the plantlet was around 14.99 after 35 days. Moreover, the plantlets grew well and there was no obvious variation of the multiplication ratio. The aseptic stem with 2 or 3 axillary buds was inoculated to 1/2MS +IBA1.0 mg·L^-1+NAA0.5 mg·L^-1(with combination of 3% sugar and 7 g·L^-1 agar)for rooting culture, the rooting rate was 96.5% in 15 day and the transplant survival rate was 95.0%.

Key words: Acacia mangium×, A. auriculiformis, efficient proliferation rate, propagation coefficient, subculture, axillary bud multiplication

CLC Number:

Shi Qiong, Hu Feng, Huang Liejian, Zhan Ni. An Efficient Axillary Bud Multiplication System of Acacia mangium × A. auriculiformis[J]. Scientia Silvae Sinicae, 2014, 50(6): 55-60.

References

顾地周,罗微,曹逊,等. 2009. 松毛翠的离体快繁体系建立及种质试管保存. 林业科学,45(7): 140-144.
(Gu D Z, Luo W, Cao X, et al. 2009. Rapid propagation system and germplasm preservation in vitro of Phyllodoce caerulea. Scientia Silvae Sinicae, 45(7): 140-144. [in Chinese] )
何文锦,黄骐,陈由强,等. 2007. 灰木相思离体培养体系的构建. 浙江林学院学报,24(6): 775-778.
(He W J, Huang Q, Chen Y Q, et al. 2007. Optimum conditions for rapid propagation with clones of Acacia implexa. Journal of Zhejiang Forestry College, 24(6): 775-778. [in Chinese] )
黄烈健,陈祖旭,张赛群,等. 2012. 马占相思优树组培快繁技术研究. 林业科学研究,25(2): 227-230.
(Huang L J, Chen Z X, Zhang S Q, et al. 2012. Tissue culture technique of Acacia mangium elite trees. Forest Research, 25(2): 227-230. [in Chinese] )
裘珍飞,曾炳山,刘英. 2002. 马占相思优树组培早期增殖速率研究. 林业科学研究,15(1): 61-65.
(Qiu Z F, Zeng B S, Liu Y. 2002. Study on the early multiplicative ratio in vitro of Acacia mangium plus trees. Forest Research, 15(1): 61-65. [in Chinese] )
沈海龙. 2005. 植物组织培养. 北京: 中国林业出版社,78-79.
石大兴,王米力,石轶松,等. 2003. 巨桉芽器官离体培养与快繁体系建立的研究. 林业科学,39(1): 69-74.
(Shi D X, Wang M L, Shi T S, et al. 2003. Rapid propagation from sprout culture in vitro of Eucalyptus grandis. Scientia Silvae Sinicae, 39(1): 69-74. [in Chinese] )
谢寅峰,张志敏,尚旭岚,等. 2011. 青钱柳茎段腋芽萌发和丛生芽增殖. 林业科学,47(1): 50-55.
(Xie Y F, Zhang Z M, Shang X L, et al. 2011. Germination of axillary buds from stem segments and proliferation of multiple shoots of Cyclocarya paliurus. Scientia Silvae Sinicae, 47(1): 50-55. [in Chinese] )
熊建勇,宗亦臣,常金财,等. 2011. 马大相思茎段的组织培养. 育苗技术,(9): 29-30.
杨增海. 1987. 园艺植物组织培养. 北京: 农业出版社,64.
张国裕,程智慧,李娟,等. 2004. 银条不定芽诱导及高效增殖体系的建立. 西北农林科技大学学报,32(2): 50-52.
(Zhang G Y, Cheng Z H, Li J, et al. 2004. Establishment of adventitious bud induction and high efficient propagation system in many flower betony ( Stachys floridana Schuttl.ex Benth.). Journal of Northwest Sci-Tech University of Agriculture and Forestry, 32(2): 50-52. [in Chinese] )
赵亚林,张方秋,张卫华,等. 2010. 不同形态氮素配比对马大杂种相思无性系幼苗生长的影响. 广东林业科技,26(4): 18-23.
(Zhao Y L, Zhang F Q, Zhang W H, et al. 2010. Effect of ratios between different forms of nitrogen on the seedling growth of Acacia mangium×A.auriculiformis Cunn.ex bench. Guangdong Forestry Science and Technology, 26(4): 18-23. [in Chinese] )
周丽华,张华通,刘颂颂,等. 1996. 相思杂种-莞屏 1号离体快速繁殖的研究. 广东林业科技,12(3): 25-28.
(Zhou L H, Zhang H T, Liu S S, et al. 1996. Micropropagation of hybrid ( Acacia mangium× A. auriculiformis). Guangdong Forestry Science and Technology, 12(3): 25-28. [in Chinese] )
Fridborg G,Eriksson T.1978. Effects of actived charcoal on growth and morphogenesis in cells cultures. Physiol Plantarum, 34(4): 306-308.
George P S,Ravishankar G A. 1997. In vitro multiplication of Vanilla planifolia using axillaries bud explants. Plant Cell Reports, 16 (7): 490-494.
Gidiman J,Moo H,Hattah M, et al. 2003. Micropropagation of Acacia mangium × A. auriculiformis hybrids in Sabah. Boiset Forets des Tropiques,(275): 77-82.
Gómez M P,Segura J. 1995. Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees. Tree Physiology, 15(9): 625-628.
Kim J Y,Lee J S. 1992. Effect of cultural conditions on rhizome growth and organogenesis of Cimbidium lancifolium native Korea in vitro. Journal of the Korean Society for Horticultural Science, 33(6): 471-476.
Nor Asmah H,Nor Hasnida H,Nashatul Zaimah N A, et al. 2011. Synthetic seed technology for encapsulation and regrowth of in vitro-derived Acacia hyrid shoot and axillary buds. African Journal of Biotechnology, 10(40): 7820-7824.
Nor Asmah H,Nor Hasnida H,Noraliza A, et al. 2012. In vitro propagation of Acacia hybrid through alginate-encapsulated shoots and axillary buds.African Journal of Biotechnology, 11(65): 12814-12817.
Otroshy M,Moradi K,Khayam Nekouei M. 2011. The effect of different cytokenins in propogation of Capsicun annuum L. by in vitro nodal cutting. Trakia Journal of Sciences, 9(3): 21-30.
Signe Tomsone,rija Galeniece,Agnese Akere, et al. 2007. In vitro propagation of Syringa vulgaris L. Biologija, 53(2): 28-31.
Thambiraj J,Paulsamy S. 2012. Rapid in vitro multiplication of the ethno-medicinal shrub, Acacia caesia (L.) Willd . (Mimosaceae) from leaf explants. Asian Pacific Journal of Tropical Biomedicine, 2(2): 618-622.
Toda T, Tajima M, Brini P B. 1995. Tissue culture of Acacia mangium, A. auriculiformis and their hybrid. Bull Natl Forest Tree Breeding, (13): 157-165.

An Efficient Axillary Bud Multiplication System of Acacia mangium × A. auriculiformis

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