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Scientia Silvae Sinicae ›› 1999, Vol. 35 ›› Issue (4): 25-30.

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ANALYSIS OF THE ORIGIN AND THE TAXONOMIC POSITION OF JUGLANS HOPEIENSIS USING RAPD MARKERS

Yanmin Wu1,Dong Pei1,Shengke Xi1,Jiarui Li2   

  1. 1. The Research Institute of Forestry, CAF Beijing 100091
    2. Department of Horticulture, Northwestern Agricultural University, Yangling 712100
  • Received:1999-03-01 Online:1999-07-25 Published:1999-07-25

Abstract:

The genomic DNA variations of J. hopeiensisJ. mandshuricaJ. regiaJ. hidsii ×J. regia and J. regia ×J. mandshurica were analyzed using random amplified polymorphism DNA(RAPD) in the present study. Twenty-eight decanucleotide arbitrary primers generated 332 RAPD bands with the size ranging from 259 bp to 3054 bp, 243 of these bands were polymorphic (73.2 %). The similarities and genetic distances among them were analyzed with Jaccard's and Nei's methods respectively. The dendrogram based on the genetic distances supports the hypothesis that J. hopeiensis originated from the nature hybrid of J. regia and J. mandshurica. In addition, the J. mandshurica gave more genetic contribution than J. regia to the hybrid. Therefore the J. mandshurica is suggested in the Sect. Cardiocaryon, which agrees with the conventional taxonomic position.

Key words: J. hopeiensis, RAPD, Origin, Taxonomic position