抗病和感病泡桐无性系组培苗对嫁接传染植原体的不同反应*

Abstract

Abstract:

The graft tests were performed under the sterile and in vitro cultured conditions using infected plantlets with phytoplasmas and seven phytoplasma-free clones of Paulownia spp. displaying different degrees of natural resistance. DAPI fluorescence microscopy and polymerase chain reaction(PCR) based on the phytoplasma 16S rRNA gene sequence were taken for the pathogen detection. The result indicates that the graft transmission of phytoplasma from diseased scion to healthy rootstock or from diseased rootstock to the healthy scion is a new efficient way to evaluate the resistance of Paulownia against phytoplasmas as well as to acquire infected clone materials. The clone C125 and XuH demonstrated strong necrotic reaction along the stem of the inoculated rootstocks from graft joints, ZH and T35-028 with moderate degree of necrosis, while QLM, C020 and C161 with slight reaction. Plant growth regulators(6-BA, NAA) added to MS medium reduced the necrotic effects, therefore increasing graft efficiency, while salicylic acid(SA) in the medium, or the removal of the roots and leaves from inoculated rootstock aggravated it. The reliable graft of diseased scion on QLM rootstock showing natural resistance did not induce witches' broom symptoms on QLM rootstock, despite the fact that pathogens were detected in the grafted rootstock plantlets by PCR. However, by grafting healthy QLM shoot on diseased rootstock, QLM scion was likely to develop typical witches' broom symptoms, which indicates some correlated resistance with that of roots or mature leaves. The phytoplasmas were graft-transmitted into all clones, except C125, and induced indistinguishable witches' broom symptoms to one another. In the phloem of infected tissues of each clone, the bright autofluorescence accumulated gradually, with the development of symptoms; and the intensity of the autofluorescence was correlated with clone's resistance to some extent

Key words: In vitro cultured plantlet of Paulownia spp., Graft transmission, Phytoplasma, Necrosis, Witches' broom, Resistance

Guozhong Tian,Xijin Zhang,Fei Luo,Suifang Zhu. RESPONSES OF RESISTANT AND SUSCEPTIBLE CLONES OF IN VITRO CULTURED PAULOWNIA TO THE GRAFT INOCULATION WITH PHYTOPLASMAS[J]. Scientia Silvae Sinicae, 1999, 35(4): 31-39.

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References 0

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RESPONSES OF RESISTANT AND SUSCEPTIBLE CLONES OF IN VITRO CULTURED PAULOWNIA TO THE GRAFT INOCULATION WITH PHYTOPLASMAS

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