嫁接于3个枣园病砧的抗病接穗枣疯植原体检测及分子变异

doi:10.11707/j.1001-7488.20211105

Abstract

Abstract:

Objective: This study aimed to detect the jujube witches'-broom(JWB) phytoplasma status of jujubes cultivated in three jujube orchards after inoculating the trees by graft, and to analyze the transmission, epidemic, heritable variation, and phylogeny of different phytoplasma strains; and reveal the relationship between different phytoplasma strains and different resistance jujube cultivars. Method: In April 2017, different resistant jujube scions were collected and grafted on the phytoplasma-infected rootstocks cultivated in three jujube orchards including Yuquanshan and Changping orchards in Beijing and Juncheng orchard in Tang county, Hebei province. The total DNA of scion and rootstock tissue samples were extracted from August to October of that year, to be used as templates for PCR amplification. Specific PCR products of 16S rDNA, 16S-23S intergenic region(SR), secA, tuf, fusA-tuf intergenic region, himA and its downstream putative protein gene were sequenced. Genetic variation and phylogenetic evolution were analyzed with obtained sequence data. Result: The symptom severity of resistant scions was generally lower than that of susceptible rootstocks. No phytoplasma was detected in all of T30 scions without symptoms of witches'-broom by direct and nested PCR and LAMP. The homology of 16S rDNA, SR, tuf and secA gene sequences of JWB phytoplasma isolates from three orchards was 100%. According to the base variation of himA and its downstream putative protein gene at 117, 154 and 190 bp sites in 38 samples, JWB strains from Junchengzhen were clustered in the T-A type, JWB strains from Yuquanshan and Liangjiagou were clustered in the C-A type, while JWB strains from Changping were clustered in the C-C type. According to the insertion or deletion of base A at 39 bp in the sequence of the fusA-tuf intergenic region in 29 samples, JWB strains from Yuquanshan and Juncheng were clustered in the TJ2(A+) type, while others were clustered in the TJ1(A-) type. There were dominant strains in each orchard that cause the sequence types of both regions(himA and its downstream putative protein gene, intergenic region of fusA-tuf genes) were mutually corroborated. Conclusion: In this study 'Junyou No.2' (Z18) jujube is for the first time identified as the high resistant cultivar to JWB. The variations of different JWB strains from two orchards have no significant correlation with the disease resistance of cultivars, while with the regional difference. The gene sequences of JWB strains from the two regions provide the evidence that JWB strains of Yuquanshan are not originated from Sihong County, Jiangsu Province. The results are helpful to reveal the genetic variation characteristics of JWB phytoplasma population and provide a theory basis for rational utilization of resistant cultivars and JWB disease control.

Key words: graft, disease resistance, jujube witches'-broom disease, phytoplasma, 16S rDNA, himA and its downstream hypothetical protein

CLC Number:

S763.7

Wenxin Zhang,Shaoshuai Yu,Guozhong Tian,He Wang,Zhengguang Ren,Shengjie Wang,Dezhi Kong,Yong Li,Caili Lin. Detection and Molecular Variation of Jujube Witches' Broom Phytoplasma in Resistant Jujube Scions Grafted on Diseased Root Stocks in Three Jujube Orchards[J]. Scientia Silvae Sinicae, 2021, 57(11): 49-58.

Figures/Tables 7

Table 1

Table 2

Symptom and phytoplasma detection results of different cultivars after grafting transmission in three jujube orchards"

样品编号 Sample No.	树号 Tree No.	症状与病级Symptom and degrees				分子检测Molecular detection
样品编号 Sample No.	树号 Tree No.	品种 Cultivar	症状 Symptom	病级 Disease degrees		PCR	Nested PCR	LAMP
YQ-SC-1	Tree 1	T27	NS	0		+	+	+
YQ-SC-2	Tree 1	T27	YL	Ⅲ		+	+	+
YQ-SC-3	Tree 1	T27	P	Ⅰ		+	+	+
YQ-RS-1	Tree 1	T19	LL、WB	Ⅴ		+	+	+
YQ-RS-2	Tree 1	T19	LL、WB	Ⅲ		+	+	+
YQ-SC-4	Tree 1	Z18	YL	i		-	-	-
YQ-RS-3	Tree 1	T19	NS	0		+	+	+
YQ-RS-4	Tree 1	T19	LL、WB	Ⅴ		+	+	+
YQ-RS-5	Tree 1	T19	WB	Ⅲ		+	+	+
YQ-SC-5	Tree 1	50	LL、WB	Ⅴ		+	+	+
YQ-SC-6	Tree 2	T30	NS	0		-	-	-
YQ-Z18	Tree 3	Z18	NS	0		-	-	+
YQ-SC-7	Tree 4	T30	NS	0		-	-	-
YQ-SC-8	Tree 5	T30	NS	0		-	-	-
YQ-RS-6	Tree 5	M13	LL、WB	Ⅴ		+	+	+
YQ-M11	Tree 6	M11	NS	0		-	-	+
YQ-SC-9	Tree 7	M11	P	Ⅱ		+	+	+
YQ-SC-10	Tree 8	T27	LL、WB	Ⅴ		+	+	+
YQ-RS-7	Tree 8	M15	LL、WB	Ⅴ		+	+	+
YQ-SC-11	Tree 9	T30	LL、WB	Ⅴ		-	-	-
YQ-RS-8	Tree 9	M15	LL、WB	Ⅴ		+	+	+
YQ-T27	Tree 10	T27	NS	0		-	-	+
JC-RS-1	Tree 11	Pozao	LL、WB	Ⅴ		+	+	+
JC-SC-1	Tree 11	T27	P	Ⅱ		+	+	+
JC-RS-2	Tree 12	Pozao	LL	Ⅰ		-	-	+
JC-SC-2	Tree 12	T27	PE	Ⅰ		+	-	+
JC-RS-3	Tree 13	Pozao	LL、WB	Ⅴ		+	+	+
JC-SC-3	Tree 13	Z18	NS	0		+	-	+
JC-RS-4	Tree 14	Pozao	LL、WB	Ⅴ		-	-	+
JC-SC-4	Tree 14	Pozao	LL、WB	Ⅴ		+	-	+
JC-ST-1	Tree 15	Pozao	LL、WB	Ⅴ		-	-	+
JC-RS-5	Tree 15	Pozao	LL、WB	Ⅴ		-	-	-
JC-SC-5	Tree 15	Z25	LL	Ⅰ		+	+	+
JC-SC-6	Tree 16	50	LL、WB	Ⅴ		-	+	+
JC-SC-7	Tree 17	87	LL、WB	Ⅴ		+	+	+
JC-SC-8	Tree 18	127	LL、WB	Ⅴ		+	+	+
JC-SC-9	Tree 19	Z25	P	Ⅰ		-	-	+
CP-SC-1	Tree 20	T27	P	Ⅱ		-	-	+
CP-RS-1	Tree 20	Dongzao	NS	0		+	+	+
CP-SC-2	Tree 21	Z18	LL、PE	Ⅰ		+	+	+
CP-RS-2	Tree 21	Dongzao	LL、WB	Ⅴ		-	+	+
CP-SC-3	Tree 22	50	NS	0		+	+	+
CP-RS-3	Tree 22	Dongzao	LL	Ⅰ		-	+	+
CP-SC-4	Tree 23	87	PE	Ⅰ		-	-	-
CP-RS-4	Tree 23	Dongzao	LL	Ⅰ		+	+	+
CP-SC-5	Tree 24	T30	NS	0		-	-	-
CP-RS-5	Tree 24	Dongzao	LL	Ⅰ		-	-	-
CP-SC-6	Tree 25	M11	P	Ⅱ		+	+	+
CP-RS-6	Tree 25	Dongzao	LL	Ⅰ		+	+	+
CP-SC-7	Tree 26	L2	P	Ⅲ		+	+	+
CP-RS-9	Tree 26	Dongzao	LL、WB	Ⅱ		-	-	+
CP-SC-9	Tree 27	T31	WB	Ⅱ		-	-	-
CP-RS-7	Tree 27	Dongzao	NS	0		+	+	+
CAF-SC-1	Tree 28	T27	NS	0		-	-	-
CAF-SC-2	Tree 28	M11	IC	Ⅰ		+	+	+
CAF-SC-3	Tree 28	Jikang	PE、IC	Ⅱ		+	+	+
CAF-RS-1	Tree 28	Unknow	NS	0		-	-	-
JWB-JSSH-QC1	Tree 29	T19	LL、WB	Ⅴ		+	+	+
JWB-JSSH-FJT	Tree 30	T19	LL、WB	Ⅴ		+	+	+
JWB-JSSH-QC2	Tree 31	T19	LL、WB	Ⅴ		+	+	+
JWB-JSSH-QC3	Tree 32	T19	LL、WB	Ⅴ		+	+	+
JWB-SDTA	Tree 33	/	LL、WB	Ⅴ		+	+	+
JWB-HBTX-LJG	Tree 34	Pozao	LL、WB	Ⅴ		+	+	+
JWB-HBTX-SJG	Tree 35	Pozao	LL、WB	Ⅴ		+	+	+
JWB-SDTA-GSZD	Tree 36	Changhongzao	LL、WB	Ⅴ	+	+	+
JWB-BJTZ-RZY	Tree 37	/	LL、WB	Ⅴ	+	+	+

Table 2

Table 3

Fig.1

Table 4

Fig.2

Fig.3

References 0

	马占鸿. 植病流行学. 北京: 科学出版社, 2010.
	Ma Z H . Plant disease epidemiology. Beijing: Science Press, 2010.
	曲泽州, 王永蕙. 中国果树志. 北京: 中国林业出版社, 1993.
	Qu Z Z , Wang Y H . Chinese fruits: Jujube. Beijing: China Forestry Publishing House, 1993.
	任争光, 王合, 林彩丽, 等. 实时荧光定量PCR(SYBR green I)检测不同抗枣疯病枣树品种嫁接接穗中的植原体浓度. 植物病理学报, 2015, 45 (5): 520- 529.
	Ren Z G , Wang H , Lin C L , et al. A real-time (SYBR Green I) PCR assay for detection and quantification of jujube witches'-broom phytoplasma in the grafted jujube cultivar scions with different resistance. Acta Phytopathologica Sinica, 2015, 45 (5): 520- 529.
	田国忠, 徐启聪, 李永, 等. 野生酸枣疯病与栽培大枣疯病发生的关系. 植物保护学报, 2009, 36 (6): 529- 536. doi: 10.3321/j.issn:0577-7518.2009.06.009
	Tian G Z , Xu Q C , Li Y , et al. Association of infected wild sour jujube Zizyphus spinosa Hu by phytoplasma with occurrence of cultivated Chinese jujube Zizyphus jujube Mill witches'broom disease. Acta Phytophylacica Sinica, 2009, 36 (6): 529- 536. doi: 10.3321/j.issn:0577-7518.2009.06.009
	田国忠, 张志善, 李志清, 等. 我国不同地区枣疯病发生动态和主导因子分析. 林业科学, 2002, 38 (2): 83- 91.
	Tian G Z , Zhang Z S , Li Z Q , et al. Dynamic of Jujube witches'broom disease and factors of great influence at ecologically different regions in China. Scientia Silvae Sinicae, 2002, 38 (2): 83- 91.
	田国忠, 张锡津, 罗飞, 等. 抗病和感病泡桐无性系组培苗对嫁接传染植原体的不同反应. 林业科学, 1999, 35 (4): 31- 39. doi: 10.3321/j.issn:1001-7488.1999.04.006
	Tian G Z , Zhang X J , Luo F , et al. Responses of resistant and susceptible clones of in vitro cultured paulownia to the graft inoculation with phytoplasmas. Scientia Silvae Sinicae, 1999, 35 (4): 31- 39. doi: 10.3321/j.issn:1001-7488.1999.04.006
	田砚亭, 王红艳, 牛辰, 等. 枣树脱除类菌原体(MLO)技术的研究. 北京林业大学学报, 1993, 15 (2): 20- 26. doi: 10.3321/j.issn:1000-1522.1993.02.001
	Tian Y T , Wang H Y , Niu C , et al. Studies on techniques of elimination mycoplasma-like organisms from Zizyphus jujuba Mill infected by jujuba witches' broom. Journal of Beijing Forestry University, 1993, 15 (2): 20- 26. doi: 10.3321/j.issn:1000-1522.1993.02.001
	王海妮, 吴云锋, 安凤秋, 等. 枣疯病和酸枣丛枝病植原体16S rDNA和tuf基因的序列同源性分析. 中国农业科学, 2007, 40 (10): 2200- 2205. doi: 10.3321/j.issn:0578-1752.2007.10.011
	Wang H N , Wu Y F , An F Q , et al. The homology analysis for sequences of 16S rDNA and tuf gene of phytoplasma from Jujube witches' broom and wild Jujube witches' broom. Scientia Agricultura Sinica, 2007, 40 (10): 2200- 2205. doi: 10.3321/j.issn:0578-1752.2007.10.011
	王合, 任争光, 潘彦平, 等. 北京市区古枣树单株种源抗枣疯病测定与抗病品种(系)筛选. 林业科学, 2018, 54 (8): 124- 132.
	Wang H , Ren Z G , Pan Y P , et al. Determination of individual jujube trees against jujube witches'-broom disease and screening of resistant varieties from the ancient individual jujube trees growing in Beijing. Scientia Silvae Sinicae, 2018, 54 (8): 124- 132.
	王景丽. 2016. 嗜盐微生物分离鉴定及耐盐运动发酵单胞菌工程菌构建. 北京: 中国农业科学研究院硕士学位论文.
	Wang J L. 2016. Isolation and identification of halophilic microorganisms and construction of salt tolerant engineered Zymomonas mobilis. Beijing: MS thesis of Chinese Academy of Agricultural Sciences. [in Chinese]
	王圣洁, 王胜坤, 林彩丽, 等. 以tuf基因为靶标的5种16Sr I组植原体环介导恒温扩增技术. 林业科学, 2017, 53 (8): 54- 63.
	Wang S J , Wang S K , Lin C L , et al. Loop-mediated isothermal amplification assay for detection of five phytoplasmas belonging to 16Sr I group based on target tuf gene. Scientia Silvae Sinicae, 2017, 53 (8): 54- 63.
	王焯, 刘秀芳, 周佩珍, 等. 枣疯病综合防治技术研究. 中国果树, 1988, (2): 40- 41.
	Wang Z , Liu X F , Zhou P F , et al. Integrated control of Jujube witches'broom. China Fruits, 1988, (2): 40- 41.
	温秀军, 郭晓军, 田国忠, 等. 几个枣树品种和婆枣单株对枣疯病抗性的鉴定. 林业科学, 2005, 41 (3): 88- 96. doi: 10.3321/j.issn:1001-7488.2005.03.015
	Wen X J , Guo X J , Tian G Z , et al. Identification of resistances of several Jujube cultivars and selected Pozao single trees against Jujube witches'broom disease. Scientia Silvae Sinicae, 2005, 41 (3): 88- 96. doi: 10.3321/j.issn:1001-7488.2005.03.015
	徐启聪, 田国忠, 王振亮, 等. 中国各地不同枣树品种上枣疯病植原体的PCR检测及分子变异分析. 微生物学报, 2009, 49 (11): 1510- 1519. doi: 10.3321/j.issn:0001-6209.2009.11.016
	Xu Q C , Tian G Z , Wang Z L , et al. Molecular characterization and variability of jujube witches'-broom phytoplasmas from different Jujube cultivars regions of China. Acta Microbiologica Sinica, 2009, 49 (11): 1510- 1519. doi: 10.3321/j.issn:0001-6209.2009.11.016
	于少帅, 林彩丽, 潘皎, 等. 泡桐丛枝和枣疯病植原体tuf基因上游序列结构、功能和遗传变异比较分析. 微生物学通报, 2016, 43 (5): 1060- 1069.
	Yu S S , Lin C L , Pan J , et al. Comparative analysis of structure, function and genetic variation of upstream sequences adjoining tuf gene in paulownia and jujube witches'-broom phytoplasmas. Microbiology China, 2016, 43 (5): 1060- 1069.
	张志善. 枣树良种引进指南. 北京: 金盾出版社, 2003.
	Zhang Z S . Introduction of quality variety of Jujube. Beijing: Jindun Publishing House, 2003.
	赵锦, 刘孟军, 周俊义, 等. 抗枣疯病种质资源的筛选与应用. 植物遗传资源学报, 2006, 7 (4): 398- 403. doi: 10.3969/j.issn.1672-1810.2006.04.005
	Zhao J , Liu M J , Zhou J Y , et al. Screening and application of Chinese Jujube germplasm with high resistance to witches'broom disease. Journal of Plant Genetic Resources, 2006, 7 (4): 398- 403. doi: 10.3969/j.issn.1672-1810.2006.04.005
	赵京芬, 胡佳续, 宋传生, 等. 北京市丰台区主栽枣品种对枣疯病抗性试验. 中国森林病虫, 2011, 30 (3): 11- 14.
	Zhao J F , Hu J J , Song C S , et al. Resistance of main Jujube cultivars in Fengtai District, Beijing against jujube witches'broom phytoplasma. Forest Pest and Disease, 2011, 30 (3): 11- 14.
	Bu J D , Peng L , Liu M J , et al. 16S rDNA sequence analysis of witches broom phytoplasma isolates from Chinese jujube in North China. Australasian Plant Pathology, 2016, 45 (1): 119- 122. doi: 10.1007/s13313-016-0396-5
	Chang K F , Hwang S F , Khadhair A H , et al. Detection and molecular characterization of an aster yellows phytoplasma in poker Statice and Queen Anne's lace in Alberta, Canada. Microbiological Research, 2004, 159 (1): 43- 50. doi: 10.1016/j.micres.2004.01.014
	Dickinson M , Hodgetts J . Phytoplasma: methods and protocols. Totowa: Humana Press, 2013.
	Hodgetts J , Boonham N , Mumford R , et al. Phytoplasma phylogenetics based on analysis of secA and 23S rRNA gene sequences for improved resolution of candidate species of 'Candidatus Phytoplasma'. International Journal of Systematic and Evolutionary Microbiology, 2008, 58 (8): 1826- 1837. doi: 10.1099/ijs.0.65668-0
	Lee I M , Hammond R W , Davis R E , et al. Universal amplification and analysis of pathogen 16S rDNA for classification and identification for mycoplasma like organisms. Phytopathology, 1993, 83, 834- 842. doi: 10.1094/Phyto-83-834
	Liu Z G , Zhao J , Liu M J . Photosynthetic responses to phytoplasma infection in Chinese jujube. Plant Physiology and Biochemistry, 2016, 105, 12- 20. doi: 10.1016/j.plaphy.2016.04.003
	Seemüller E , Schneider B . Differences in virulence and genomic features of strains of 'Candidatus phytoplasma mali', the apple proliferation agent. Phytopathology, 2007, 97 (8): 964- 970. doi: 10.1094/PHYTO-97-8-0964
	Wang J , Song L , Jiao Q , et al. Comparative genome analysis of jujube witches 1-broom phytoplasma, an omgcte pathogen that causes jujube withes'-broom disease. BMC Genomics, 2018, 19 (1): 689. doi: 10.1186/s12864-018-5075-1
	Weintraub P G , Jones P . Phytoplasmas: genomes, plant hosts and vectors. Phytoplasmas Genomes Plant Hosts & Vectors, 2009, 156 (1): 8- 14.

抗性Resistance	健康接穗Scion	砧木/根蘖Rootstock/Stool shoot
高抗High resistance(HR)	骏枣Z. jujuba ‘Junzao’(T27)、壶瓶枣Z. jujuba ‘Hupingzao’(Z17)
普抗General resistance(R)	洪赵小枣Z. jujuba ‘Hongzhaoxiaozao’(T30)、蜂蜜罐枣Z. jujuba Fengmiguan(M11)、北京古树87号Beijing ancient tree No.87(87)、北京古树127号Beijing ancient tree No.127(127)
中抗Medium resistance(MR)	北京古树50号Beijing ancient tree No.50(50)
感病Susceptibility(S)		泗洪大枣Z. jujuba ‘Sihongdazao’(T19)、酸枣Z. spinosa (Suanzao)、婆枣Z. jujuba ‘Pozao’(Pozao)
高感High susceptibility(HS)	濮阳冬枣Z. jujuba ‘puyangdongzao’(L2)、冀抗Z. jujuba ‘Jikang No.1’(JK)	襄汾圆枣Z. jujuba Xiangfenyuanzao(M15)、晋枣Z. jujuba ‘Jinzao’(M13)、冬枣Z. jujuba ‘Dongzao’(Dongzao)
未知Unknown	石西大酸枣Z. spinosa ‘Shixi’(Z25)、骏优2号Z. jujuba ‘Junyou No.2’(Z18)

基因Genes	扩出数(条) Amplification number	扩出率 Amplification rate(%)	变异位点 Variation site	变异率 Variation rate(%)
16Sr	42	63.6	0	0
SR	42	63.6	0	0
SecA	31	47.0	0	0
Tuf	32	48.5	0	0
himA及其下游假定蛋白基因 himA and its downstream hypothetical protein gene	38	57.6	3	9.6
fusA-tuf基因间区 fusA-tuf intergenic region	29	43.9	1	18.9

[1]	Qionglian Wan,Lianchun Wang,Quan Wang,Xingping Xu,Fan Su,Jing Zhao,Hong Cai. Molecular Identification and Disease Investigation of Trema levigata Witches'-Broom Phytoplasma [J]. Scientia Silvae Sinicae, 2021, 57(5): 195-201.
[2]	Zhou Naifu, Song Xiaobo, Zhang Junpei, Chang Yingying, Pei Dong. Histological Mechanism of Bud Grafting in Walnut [J]. Scientia Silvae Sinicae, 2019, 55(6): 37-43.
[3]	Liang Senmiao, Zhang Shuwen, Ren Haiying, Zheng Xiliang, Qi Xingjiang. The Interspecific Grafting and Phylogenetic Analysis of Myrica cerifera and Myrica rubra [J]. Scientia Silvae Sinicae, 2019, 55(4): 171-177.
[4]	Yang Anna, Lu Yunfeng, Zhang Junhong, Wu Jiasen, Xu Jinliang, Tong Zaikang. Changes in Soil Nutrients and Acidobacteria Community Structure in Cunninghamia lanceolata Plantations [J]. Scientia Silvae Sinicae, 2019, 55(1): 119-127.
[5]	Fu Bing, Ye Xia, Wang Huiyu, Chen Peng, Li Jidong, Zheng Xianbo, Tan Bin, Feng Jiancan. Identification of WRKY Transcription Factors in Jujube and Their Responses to ‘Candidatus Phytoplasma ziziphi’ and Salicylic Acid or Methyl Jasmonate Treatments [J]. Scientia Silvae Sinicae, 2018, 54(8): 65-78.
[6]	Wang He, Ren Zhengguang, Pan Yanping, Feng Shukuai, Lin Caili, Chang Enzhong, Yu Shaoshuai, Tian Guozhong. Determination of Individual Jujube Trees against Jujube Witches'-Broom Disease and Screening of Resistant Varieties from the Ancient Individual Jujube Trees Growing in Beijing [J]. Scientia Silvae Sinicae, 2018, 54(8): 124-132.
[7]	Wang Shengjie, Wang Shengkun, Lin Caili, Yu Shaoshuai, Wang Laifa, Piao Chungen, Guo Minwei, Tian Guozhong. Loop-Mediated Isothermal Amplification Assay for Detection of Five Phytoplasmas Belonging to 16SrⅠ Group Based on Target tuf Gene [J]. Scientia Silvae Sinicae, 2017, 53(8): 54-63.
[8]	Tang Dan, Jiang Xibing, Gong Bangchu, Liu Tongxiang, Xu Yan, Wu Kaiyun. Early Selection of Interstocks for Improving Grafting Compatibility in Diospyros kaki ‘Fuyu’ [J]. Scientia Silvae Sinicae, 2017, 53(5): 54-62.
[9]	Yu Shaoshuai, Li Yong, Ren Zhengguang, Song Chuansheng, Lin Caili, Piao Chungen, Tian Guozhong. Multilocus Sequence Analysis for Revealing Finer Genetic Variation and Phylogenetic Interrelatedness of Phytoplasma Strains in 16SrI Group in China [J]. Scientia Silvae Sinicae, 2017, 53(3): 105-118.
[10]	Chen Panfei, Ren Yachao, Zhang Jun, Wang Jinmao, Yang Minsheng. Expression and Transportation of Bt Toxic Protein in 8-Year-Old Grafted Transgenic Poplar [J]. Scientia Silvae Sinicae, 2016, 52(7): 46-52.
[11]	Song Chuansheng, Hu Jiaxu, Lin Caili, Ren Zhengguang, Geng Xiansheng, Tian Guozhong. Thymidylate Kinase Gene Polymorphism of Two Strains of Paulownia Witches’-Broom Phytoplasma [J]. Scientia Silvae Sinicae, 2014, 50(8): 108-118.
[12]	Sun Jingshuang, Li Shaofeng, Dong Chenxi, Sun Changzhong. Research Progress and Prospect of Long Distance Transmission of RNA Molecules in Grafted Plants [J]. Scientia Silvae Sinicae, 2014, 50(11): 158-165.
[13]	Wang Tao;Li Yong;Piao Chungen;Wang Laifa;Zhu Tianhui. Diversity and Seasonal Fluctuations of Culturable Endophytic Bacteria Isolated from Two Poplars [J]. , 2013, 49(5): 135-146.
[14]	Ouyang Lejun;Liu Yuan;Huang Zhenchi;Zeng Fuhua. Transformation of TSRF 1 into Eucalyptus urophylla and the Broad-Spectrum Disease Resistance of the Transgenic Plant to Diseases [J]. Scientia Silvae Sinicae, 2013, 49(4): 46-53.
[15]	Hu Jiaxu;Song Chuansheng;Lin Caili;Geng Xiansheng;Tian Guozhong. Sequencing the Full-Length DNA and the Molecular Characterization of Four Plasmids from Plant Pathogens of Phytoplasma Disease [J]. Scientia Silvae Sinicae, 2013, 49(4): 90-97.

Detection and Molecular Variation of Jujube Witches' Broom Phytoplasma in Resistant Jujube Scions Grafted on Diseased Root Stocks in Three Jujube Orchards

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 0

Related Articles 15

Recommended Articles

Metrics

Comments

序列型 Sequence type	位点Site			样品序列Sequences
序列型 Sequence type	117	154	190	样品序列Sequences
C-C	C	/	C	JWB-JSSH-QC1、JWB-JSSH-FJT、JWB-JSSH-QC2、JWB-JSSH-QC3、JWB-SDTA、JWB-SDTA-GSZD、JWB-BJTZ-RZY、CP-SC-2、CP-RS-2、CP-SC-7、CP-RS-9
C-A	C	/	A	JWB-HBTX-LJG、YQ-SC-1、YQ-SC-2、YQ-SC-3、YQ-RS-1、YQ-RS-3、YQ-RS-4、YQ-RS-5、YQ-SC-5、YQ-RS-8、YQ-SC-9、YQ-SC-10、YQ-RS-7、JC-ST-1、JC-SC-7、CP-SC-6、CP-RS-6
T-A	T	/	A	YQ-RS-6、JC-RS-1、JC-SC-1、JC-SC-5、JC-SC-6、、JC-SC-8、JC-RS-4、JC-SC-9
T-A	T	A	A	JC-RS-3