基于抑制消减杂交技术筛选AM真菌与紫穗槐共生相关基因

doi:10.11707/j.1001-7488.20141109

Abstract

Abstract:

In this study, the suppression subtractive hybridization (SSH) method was employed to screen the differential expressed genes in Amorpha fruticosa roots induced by AMF during the colonization process. The results showed that 47 unigenes were obtained, and 35 of them could be aligned with known function genes in GenBank through Blast. According to GO classified information, Blast annotation and related literatures, the 35 unigenes were assigned into eight categories including stress and defense (24%), substance metabolism (17%), energy metabolism (14%), protein synthesis (11%), signaling (11%), cellular structre/cytoskeleton (11%), transcription related (6%), protein folding and degradation related (6%). Among them, stress and defense genes, and metabolism genes occupied the largest fraction, implying stress and defense related genes and substance metabolism related genes should play an important role in the symbiosis process between A. fruticosa and AMF. These genes are widely involved in the biological process of life activity, thus, the process of AMF and A. fruticosa symbiosis is a system involving a variety of biological activities.

Key words: Amorpha fruticosa, arbuscular mycorrhizal fungi, suppression subtractive hybridization, symbiosis related genes

CLC Number:

S718.81

Song Fuqiang, Kong Xiangshi, Li Jize, Chang Wei. Screening the Related Genes in the AM Fungi and Amorpha fruticosa Symbiosis with the Suppression Subtractive Hybridization Technique[J]. Scientia Silvae Sinicae, 2014, 50(11): 64-74.

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Screening the Related Genes in the AM Fungi and Amorpha fruticosa Symbiosis with the Suppression Subtractive Hybridization Technique

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