松天牛小首螨的研究Ⅱ.雌成螨的移动和趋性

doi:10.11707/j.1001-7488.20220815

Abstract

Abstract:

Objective: The mobility and tropism of natural enemy insects or mites are important factors that affect their biological control effects on pests. Understanding the mobility and tropism of Paracarophenax alternatus, a mite, can provide important basis for scientific evaluation of its biological control potential and formulation of its field protection and utilization methods for biological control. Method: In this study, the translational ability, vertical tropism, ascending ability, phototropism, and Monochamus alternatus adults and eggs tropism of the female adult of P. alternatus were investigated through micro-photography, behavioral measurement and control experiments. Result: The female adult of P. alternatus had strong mobility. In the horizontal direction, the average crawling speed of female adult mites from 0- to 8-day-old was 2.17 cm ·min^-1. The 0-day-old female adult mites had the highest crawling speed up to 2.63 cm·min^-1, while the crawling speed of the 8-days-old female adult mites was the slowest, with only 1.32 cm·min^-1. With the increase of the day-age, the crawling speed showed a significant downward trend; In the vertical direction, the female adult mites had significant upward-crawling habit. The upward-crawling individuals within 1-3 hours were 9.7 times that of down-crawling ones. After releasing mites at the base of bamboo stalk in the wild for 1 to 3 days, the female adult mites ascending to 5, 7, 8 and 9 m and above accounted for 26.75%-41.75%, 18.15%-31.35%, 12.00%-26.95% and 7.30%-20.75% of the total mites, respectively. At the same time, the female adult of P. alternatus showed significant phototaxis and tropism to M. alternatus adult and eggs. In the range of the light intensity from 400 lx to 2 000 lx, the selection rate of the mites to light area was 8.1 times that of it to dark area. P. alternatus' selection rate to M. alternatus male, female and eggs was significantly higher than that of the blank control under the conditions of illumination, shading or M. alternatus illuminated with blank control shaded. Conclusion: The female adult of P. alternatus has strong mobility, upward-crawling habit, significant phototaxis and host tropism. These characteristics are helpful for the mite to efficiently search for the host eggs or gather on the body surface of M. alternatus adults to phoresy in the wild. Hence, it is a biological control factor with great application potential. In future biological control practices, it is recommended to encapsulate the mites in black or dark containers and then release from the base of pine trunks.

Key words: Paracarophenax alternatus, mobility, tropism, Monochamus alternatus, Bursaphelenchus xylophilus

CLC Number:

S718.7

Junnan Li,Runkai Chen,Yu Fu,Mengling Cai,Bingrong Huang,Yun Xu,Songqing Wu,Feiping Zhang. Research on Paracarophenax alternatusⅡ. Mobility and Tropism of Female Adults[J]. Scientia Silvae Sinicae, 2022, 58(8): 149-156.

Figures/Tables 6

Fig.1

Table 1

Table 2

Fig.2

Table 4

Fig.3

References 0

	蔡梦玲. 松材线虫在树干中的分布与松墨天牛成虫历期影响因素分析. 福州: 福建农林大学, 2019.
	Cai M L . Distributing PWN in Pinus massoniana and analysis of environmental factors impacting adulthood duration of Monochamus alternatus Hope. Fuzhou: Fujian Agriculture and Forestry University, 2019.
	陈龙, 林强, 李俊楠, 等. 松墨天牛诱捕器空间位置的野外对比试验. 福建林学院学报, 2014, 34 (1): 11- 14.
	Chen L , Lin Q , Li J N , et al. Comparison of efficacies for Monochamus alternatus adult caught by panel traps baited with APF-Ⅰ chemical attractant placed at different field positions. Journal of Fujian College of Forestry, 2014, 34 (1): 11- 14.
	郝德君, 张永慧, 戴华国, 等. 松墨天牛对寄主树木的产卵选择. 昆虫学报, 2005, 48 (3): 460- 464. doi: 10.3321/j.issn:0454-6296.2005.03.024
	Hao D J , Zhang Y H , Dai H G , et al. Oviposition preference of monochamus alternatus Hope (Coleoptera: Cerambycidae) to host plants. Acta Entomologica Sinica, 2005, 48 (3): 460- 464. doi: 10.3321/j.issn:0454-6296.2005.03.024
	康文通, 汤陈生, 梁农, 等. 应用管氏肿腿蜂林间防治松墨天牛. 福建农林大学学报(自然科学版), 2008, 37 (6): 575- 579.
	Kang W T , Tang C S , Liang N , et al. Releasing Scleroderma guani to control Monochamus alternatus. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2008, 37 (6): 575- 579.
	李俊楠, 朱禹臻, 陈润恺, 等. 松天牛小首螨的研究Ⅰ. 生物学特性.林业科学, 2022, 58 (6): 79- 87.
	Li J N , Zhu Y Z , Chen R K , et al. Research on paracarophenax alternatus Ⅰ. Biological Characteristics. Scientia Silvae Sinicae, 2022, 58 (6): 79- 87.
	李孟楼, 李有忠, 雷琼, 等. 释放花绒寄甲卵对光肩星天牛幼虫的防治效果. 林业科学, 2009, 45 (4): 78- 82.
	Li M L , Li Y Z , Lei Q , et al. Biocontrol of Asian longhorned bettle larva by releasing eggs of Dastarcus helophoroides (Coleoptera: Bothrideridae). Scientia Silvae Sinicae, 2009, 45 (4): 78- 82.
	皮忠庆, 王福维, 高立军, 等. 管氏肿腿蜂防治青杨天牛试验. 中国森林病虫, 2001, 20 (6): 20- 22.
	Pi Z Q , Wang F W , Gao L J , et al. Experiment on control of Saperda populnea with Scleroderma guani. Forest Pest and Disease, 2001, 20 (6): 20- 22.
	王功桂, 周灵会, 王长旭, 等. 管氏肿腿蜂防治松墨天牛技术. 中国森林病虫, 2004, 23 (3): 32- 34.
	Wang G G , Zhou L H , Wang C X , et al. Techniques of using Scleroderma guani against Monochamus alternatus. Forest Pest and Disease, 2004, 23 (3): 32- 34.
	王小军, 郭桂凤. 应用管氏肿腿蜂防治双条杉天牛技术研究. 中国森林病虫, 2007, 26 (4): 28- 29.28-29, 34
	Wang X J , Guo G F . Control techniques against Semanotus bifasciatus with Scleroderma guani. Forest Pest and Disease, 2007, 26 (4): 28- 29.28-29, 34
	姚万军, 杨忠岐. 利用管氏肿腿蜂防治光肩星天牛技术研究. 环境昆虫学报, 2008, 30 (2): 127- 134.
	Yao W J , Yang Z Q . Studies on biological control of Anoplophora glabripennis (Coleoptera: Cerambycidae) with a parasitoid, Sclerodermus guani (Hymenoptera: Bethylidae). Journal of Environmental Entomology, 2008, 30 (2): 127- 134.
	余德亿, 张艳璇, 唐建阳, 等. 捕食螨在我国农林害螨生物防治中的应用. 昆虫知识, 2008, 45 (4): 537- 541.
	Yu D Y , Zhang Y X , Tang J Y , et al. The application of predatory mites in biological control of agro-forest spider mites in China. Chinese Bulletin of Entomology, 2008, 45 (4): 537- 541.
	张星耀, 骆有庆. 中国森林重大生物灾害. 北京: 中国林业出版社, 2003.
	Zhang X Y , Luo Y Q . Major forest diseases and insect pests in China. Beijing: China Forestry Publishing House, 2003.
	赵锦年, 应杰. 松墨天牛取食为害与松树枯死关系的研究. 林业科学, 1989, 25 (5): 432- 438.
	Zhao J N , Ying J . A study on relationship between the damage of pine sawyer beetle (Monochamus alternatus) and pine dying. Scientia Silvae Sinicae, 1989, 25 (5): 432- 438.
	Xu Y , Li Y C , Huang B R , et al. First record of the genus Paracarophenax (Acari: Acarophenacidae) from China, with description of a new species. Systematic & Applied Acarology, 2018, 23 (12): 2411- 2419.

日龄 Days-age/d	极小值 Min	极大值 Max	95%置信区间 95% Confidence interval for mean		平均平移速度 Mean crawling speed (Mean ± SE)
日龄 Days-age/d	极小值 Min	极大值 Max	下限 Lower bound	上限 Upper bound	平均平移速度 Mean crawling speed (Mean ± SE)
0	1.20	4.44	2.49	2.76	2.63 ± 0.07a
1	1.14	3.36	2.12	2.45	2.28 ± 0.08ab
2	1.14	4.20	2.32	2.73	2.52 ± 0.10a
3	0.84	4.14	2.42	2.84	2.63 ± 0.11a
4	0.90	3.60	2.14	2.57	2.35 ± 0.11ab
5	0.84	3.42	1.96	2.37	2.16 ± 0.10b
6	0.60	3.24	1.60	2.02	1.81± 0.11c
7	0.36	3.06	1.17	1.50	1.33 ± 0.08d
8	0.42	2.34	1.18	1.45	1.32 ± 0.07d
总体 Total	0.36	4.44	2.09	2.24	2.17 ± 0.04

时间 Time/h	趋向选择 Vertical tropism	松天牛小首螨数量 Number of P. alternatus(Mean ± SE)	极小值 Min	极大值 Max	95%置信区间 95% Confidence interval for mean
时间 Time/h	趋向选择 Vertical tropism	松天牛小首螨数量 Number of P. alternatus(Mean ± SE)	极小值 Min	极大值 Max	下限 Lower bound	上限 Upper bound
1	向上 Up	17.6 ± 2.3	11	25	11.2	24.0
1	向下 Down	1.4 ± 0.8	0	4	0.0	3.5
2	向上 Up	19.8 ± 2.0	16	26	14.2	25.3
2	向下 Down	2.8 ± 1.2	0	6	0.0	6.0
3	向上 Up	18.2 ± 2.1	11	24	12.3	24.1
3	向下 Down	1.6 ± 0.7	0	4	0.0	3.5
总体 Total	向上 Up	18.5 ± 1.2	11	26	16.0	21.1
总体 Total	向下 Down	1.9 ± 0.5	0	6	0.9	3.0

光照强度 Light intensity/lx	亮 Light			暗 Dark
	选择率 Selection percentage (Mean ± SE)	95%置信区间 95% Confidence interval for mean		选择率 Selection percentage (Mean ± SE)	95%置信区间 95% Confidence interval for mean
	选择率 Selection percentage (Mean ± SE)	下限 Lower bound	上限 Upper bound	选择率 Selection percentage (Mean ± SE)	下限 Lower bound	上限 Upper bound
400	40.8 ± 6.4	22.9	58.7	4.4 ± 1.2	1.2	7.6
600	41.2± 9.1	16.0	66.4	2.8 ± 0.5	1.4	4.2
800	39.6 ± 3.4	30.1	49.1	8.8 ± 1.6	4.3	13.3
1 000	35.2 ± 2.0	29.8	40.6	2.8 ± 0.5	1.4	4.2
1 200	36.8 ± 2.8	29.0	44.6	8.8 ± 2.7	1.4	16.2
1 400	33.6 ± 7.0	14.3	53.0	7.6 ± 1.9	2.2	13.0
1 600	36.4 ± 3.1	27.9	44.9	4.0 ± 1.7	0.0	8.7
1 800	36.0 ± 2.3	29.7	42.3	2.0 ± 0.9	0.0	4.5
2 000	51.2 ± 7.1	31.5	70.9	2.0 ± 0.9	0.0	4.5
总体 Total	39.0 ± 1.8	35.4	42.6	4.8 ± 0.6	3.6	6.0

[1]	Xiaolei Ding,Qingtong Wang,Sixi Lin,Ruiwen Zhao,Yue Zhang,Jianren Ye. Analysis of Genetic Variations of Bursaphelenchus xylophilus Populations between Guangdong and Jiangsu Provinces with SNP Marker [J]. Scientia Silvae Sinicae, 2022, 58(8): 1-9.
[2]	Yefan Cao,Xizhuo Wang,Laifa Wang,Xiang Wang,Ming Xu,Shengrong Su,Wei Guo. Effects of Different Virulence Isolates of Bursaphelenchus xylophilus on Aetivitres Early Enzymes in Pinus koraiensis [J]. Scientia Silvae Sinicae, 2022, 58(8): 10-17.
[3]	Junnan Li,Yuzhen Zhu,Runkai Chen,Yu Fu,Mengling Cai,Bingrong Huang,Yun Xu,Songqing Wu,Feiping Zhang. Research on Paracarophenax alternatus Ⅰ. Biological Characteristics [J]. Scientia Silvae Sinicae, 2022, 58(6): 79-87.
[4]	Minjia Wang,Jianren Ye,Yusheng Tu,Fangping Du. Screening of Wood Rot Fungi for Treating the Infected Wood by Bursaphelenchus xylophilus [J]. Scientia Silvae Sinicae, 2021, 57(6): 93-102.
[5]	Jiangxia Ye,Jingwen Wang,Mingsha Zhang,Ruliang Zhou,Lei Shi. Risk Pattern Analysis of Hyphantria cunea Based on Spatial Matrix Model and 0-1 Measure [J]. Scientia Silvae Sinicae, 2021, 57(1): 140-152.
[6]	Xiaosui Wen,Dunfu Song,Zhongqi Yang,Zhonghui Wang,Mingqing Shi. Relationships between the Emergence of Dastarcus helophoroides (Coleoptera: Bothrideridae) and the Emergence of the Host Monochamus alternatus (Coleoptera: Cerambycidae) in Pinus massoniana Forests [J]. Scientia Silvae Sinicae, 2020, 56(9): 193-200.
[7]	Yechen Li,Yajie Guo,Xiaoqian Weng,Xianyun Lin,Jinliang Chi,Hongying Chen,Songqing Wu,Feiping Zhang. Influence of Landscape Patterns on Population Density of Monochamus alternatus (Coleoptera: Cerambycidae) [J]. Scientia Silvae Sinicae, 2020, 56(8): 80-88.
[8]	Lihua Zhu,Xinyue Zhang,Xinrui Xia,Yu Wan,Shanjun Dai,Jianren Ye. Pathogenicity of Aseptic Bursaphelenchus xylophilus on Pinus massoniana [J]. Scientia Silvae Sinicae, 2020, 56(7): 63-69.
[9]	Huijing Fu,Xia Hu,Songqing Wu,Rong Wang,Guanghong Liang,Shiguo Huang,Feiping Zhang. Culture Conditions and Lignin-Degrading Function of Serratia marcescens Living in the Larval Gut of Monochamus alternatus [J]. Scientia Silvae Sinicae, 2020, 56(2): 106-115.
[10]	Yefan Cao,Laifa Wang,Xizhuo Wang,Jiehong Fan. Pathogenicity of Bursaphelenchus xylophilus to Larix olgensis Seedlings [J]. Scientia Silvae Sinicae, 2020, 56(11): 108-115.
[11]	Ran He,Xia Cui,Yue Ying,Liangjian Qu,Ruizhen Wang,Yong Zhang. Screening and Identification of Beauveria bassiana Strains for Biocontrol of Monochamus alternatus Adults (Coleoptera: Cerambycidae) [J]. Scientia Silvae Sinicae, 2020, 56(10): 129-134.
[12]	Yuting Mao,Tao Ma,Laijiao Lan,Xiujun Wen. Advances in Esteya vermicola, A Potential Biocontrol Fungus for Pine Wood Nematode [J]. Scientia Silvae Sinicae, 2020, 56(1): 180-190.
[13]	Ye Jianren. Epidemic Status of Pine Wilt Disease in China and Its Prevention and Control Techniques and Counter Measures [J]. Scientia Silvae Sinicae, 2019, 55(9): 1-10.
[14]	Lin Li, Zhou Lei, Pan Jun, Kang Lipeng, Ye Jianren, Zhu Lihua. Pathogenicity of Aseptic and Germ-Carrying Bursaphelenchus xylophilus on Pinus densiflora [J]. Scientia Silvae Sinicae, 2017, 53(5): 82-87.
[15]	Xu Jianxiu, Wu Xiaoqin, Ye Jianren, Zhu Lihua, Wu Jing. Development, Maturation and Germination of Somatic Embryo of Nematode-Resistant Pinus densiflora [J]. Scientia Silvae Sinicae, 2017, 53(12): 41-49.

Research on Paracarophenax alternatusⅡ. Mobility and Tropism of Female Adults

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 0

Related Articles 15

Recommended Articles

Metrics

Comments