不同致病力松材线虫对红松早期防御酶活性影响

doi:10.11707/j.1001-7488.20220802

Abstract

Abstract:

Objective: This study aims to explore the relationships between pathogenicity of different isolates of Bursaphelenchus xylophilus and the amplitude of defensive enzymes in the host plant, by investigating the pathogenicity of different isolates, and the activity changes of superoxide dismutase (SOD), catalase (CAT), phenylalanine ammonia-lyase (PAL), and polyphenol oxidation (POD) in Pinus koraiensis seedlings after inoculated with B. xylophilus. This study would provide reference for the prevention and control of the pine wood nematode disease. Method: Four-year-old P. koraiensis seedlings were inoculated separately with three different isolates (QH-1, NM-1 and CM-1) of B. xylophilus, and each plant was inoculated with 2 000 nematodes. The leaves were collected every day from next day to day 7 after inoculation, and used to analysis the activity change of SOD, CAT, PAL and POD in P. koraiensis seedlings. The pathogenicity and the external symptom were observed daily. In 35 days after the inoculation, the morbidity was recorded and nematodes were extracted from wilt seedlings, to analyze the difference in pathogenicity among QH-1, NM-1, and CM-1. Result: The activity change of SOD, CAT, PAL and POD in P. koraiensis seedlings inoculated with QH-1, NM-1, and CM-1 was obviously different, and the amplitude of enzyme activity change of the seedlings with QH-1 treatment was greater than that with NM-1 and CM-1 treatments. All three isolates of B. xylophilus were able to cause wilt to P. koraiensis seedlings, with QH-1 being more virulent than other two isolates. The strong virulent strains had greater change amplitudes of enzyme activity in inoculated seedlings. Conclusion: The virulence of B. xylophilus can be determined by measuring and analyzing the amplitude of enzyme activity change of infected P. Koraiensis seedlings The strong virulent strains cause greater changes in the amplitudes of enzyme activity in inoculated seedlings.

Key words: Bursaphelenchus xylophilus, Pinus koraiensis, defensive enzyme, virulence

CLC Number:

S763

Yefan Cao,Xizhuo Wang,Laifa Wang,Xiang Wang,Ming Xu,Shengrong Su,Wei Guo. Effects of Different Virulence Isolates of Bursaphelenchus xylophilus on Aetivitres Early Enzymes in Pinus koraiensis[J]. Scientia Silvae Sinicae, 2022, 58(8): 10-17.

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Effects of Different Virulence Isolates of Bursaphelenchus xylophilus on Aetivitres Early Enzymes in Pinus koraiensis

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线虫编号Inoculum No.	接种株数Number of inoculation	首次发病时间First time of wilt symptoms observed/	发病率Morbidity rate (%)	感病指数Disease-infected index	再分离线虫量Recovery of nematodes
QH-1	10	10	100	100	28 776±4 774a
NM-1	10	15	60	55	19 290±3 502b
CM-1	10	15	40	30	19 737±3 501b
CK	10	—	0	0	0