氟离子和碳酸氢根对构树幼苗生长和碳代谢的影响

doi:10.11707/j.1001-7488.20210606

Abstract

Abstract:

Objective: The study was aimed to investigate the effects of fluoride and bicarbonate on the growth and carbon metabolism of Broussonetia papyrifera seedlings,and to reveal the mechanism of the effects of fluoride ions on plant photosynthesis and sugar metabolism in karst ecological region,in order to provide a theoretical basis for forest vegetation management and restoration in fluorine rich areas. Method: B. papyrifera seedlings were treated with Hoagland nutrient solution added with NaF and NaHCO₃ in 4 ways: control,3 mmol·L^-1 NaF,3 mmol·L^-1 NaHCO₃ and 3 mmol·L^-1 NaF+3 mmol·L^-1 NaHCO₃. The growth parameters,photosynthetic parameters,phosphofructokinase(PFK),glucose-6-phosphate dehydrogenase(G6PDH) and carbonic anhydrase(CA) enzyme activities of B. papyrifera seedlings were measured. Result: 1) Addition of F^- significantly inhibited the growth and photosynthetic capacity of plants,and transferred glucose metabolism from glycolysis pathway to pentose phosphate pathway. When HCO₃^- was not added at the same time,F^- had no significant effect on CA activity in leaves; but when HCO₃^- was added at the same time,F^- significantly inhibited CA activity in leaves. 2) Rhizospheric HCO₃^- significantly promoted plant growth and photosynthetic capacity,increased total glucose metabolism and CA enzyme activity. However,F^- inhibited the positive effects of HCO₃^- on plants,consequently significantly reduced growth,photosynthesis and glucose metabolism,but significantly increased G6PDH activity. Conclusion: In conclusion,the higher concentration of F^- in the environment significantly inhibits the growth of B. papyrifera seedlings,and its adverse effects on the growth of B. papyrifera are reflected in the inhibition of photosynthetic process and the distribution of excessive carbohydrate metabolic substrates to pentose phosphate pathway. The appropriate amount of rhizospheric HCO₃^- provides additional photosynthetic metabolic substrate for plant growth,and improves the glucose metabolism level of plants,which improves the stress resistance and growth ability of plants. Under the HCO₃^- and F^- treatment,the favorable effect of HCO₃^- on plant growth was reduced by F^-,mainly due to the destruction and inhibition of the structure and activity of various enzymes in plant photosynthetic system,and it may also be related to the competition between HCO₃^- and F^- during root absorption.

Key words: Broussonetia papyrifera, F^-, HCO₃^-, photosynthesis, glucose metabolism, carbonic anhydrase, phosphofructokinase, glucose-6-phosphate dehydrogenase

CLC Number:

S718.43

Yao Seedlings,Yanyou Wu. Effects of Fluoride and Bicarbonate on Growth and Carbon Metabolism of Broussonetia papyrifera Seedlings[J]. Scientia Silvae Sinicae, 2021, 57(6): 56-63.

Figures/Tables 6

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References 0

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生长指标 Growth parameters	对照 Control	3 mmol·L^-1 HCO₃^-	3 mmol·L^-1 F^-	3 mmol·L^-1 F^-+ 3 mmol·L^-1 HCO₃^-
株高Height(H)/cm	12.73±0.15 b	13.07±0.31 a	12.30±0.26 b	12.60±0.06 b
基径Basal diameter (D_b)/mm	1.58±0.01 ab	1.43±0.03 b	1.52±0.02 ab	1.66±0.02 a
叶片数Number of leaves (N)	8.33±0.58 a	8.67±0.58 a	8.00±0 a	7.66±0.58 a
叶宽≥70 mm的叶片数Number of blades more than 70 mm wide (N₇₀)	2.33±0.58 b	3.00±0 a	2.67±0.58 a	3.00±0 a

生长指标 Growth parameters	对照 Control	3 mmol·L^-1 HCO₃^-	3 mmol·L^-1 F^-	3 mmol·L^-1 F^-+ 3 mmol·L^-1 HCO₃^-
株高Height(H)/cm	16.92±0.68 b	18.41±0.82 a	13.75±0.88 d	15.03±0.79 c
基径Basal diameter (D_b)/mm	2.01±0.04 b	2.11±0.03 a	1.65±0.04 d	1.87±0.06 c
叶片数Number of leaves (N)	9.80±0.40 b	11.00±0.63 a	7.80±0.40 c	8.00±0.00 c
叶宽≥70 mm的叶片数 Number of blades more than 70 mm wide (N₇₀)	4.00±0.00 b	5.20±0.40 a	2.60±0.40 c	4.20±0.40 b
气孔导度Stomatal conductance (g_s) /(mol·m^-2s^-1)	0.16±0.02 a	0.15±0.01 a	0.11±0.02 b	0.11±0.01 b
净光合速率Net photosynthetic rate (P_n) /(μmol·m^-2s^-1)	5.87±0.04 b	6.12±0.03 a	2.73±0.07 d	3.39±0.06 c

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Effects of Fluoride and Bicarbonate on Growth and Carbon Metabolism of Broussonetia papyrifera Seedlings

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