土壤镉污染对香樟幼苗光合和生理特性的影响

doi:10.11707/j.1001-7488.20200619

Abstract

Abstract:

Objective: In this study,the effect of different concentrations of cadmium pollution on physiological and biochemical characteristics of Cinnamomum camphora seedlings was investigated,which aims to reveal the response mechanism of photosynthetic and other physiological characteristics of C. camphora under cadmium stress,so as to provide data accumulation and scientific basis for the remediation of contaminated soil. Method: In this study,an indoor pot experiment was conducted with one-year-old C. camphora seedlings that were subjected to 4 treatments of different contents of cadmium in contaminated soil,including 0 mg·kg^-1 (CK),1 mg·kg^-1 (Cd1),10 mg·kg^-1 (Cd2) and 30 mg·kg^-1 (Cd3),and each treatment had 5 replicates. Samples were collected and analyzed on days 0,15,30,and 60. The photosynthetic characteristics parameters,the contents of chlorophyll,proline,soluble sugar,soluble protein,malondialdehyde (MDA),and the activities of superoxide dismutase (SOD) and peroxidase (POD) of C. camphora seedlings were tested to explore the tolerance of C. camphora to cadmium and its potential and feasibility for phytoremediation. Result: The net photosynthetic rate (P_n) of C. camphora leaves did not change significantly under different concentrations of cadmium pollution in 0-15 days. P_n of the seedlings treated with contaminated soil for 30 days and 60 days was lower than that of the control,and P_n of seedlings treated with high Cd²⁺concentration (Cd3) was the lowest with an average deceases of approximate 22.33% compared with that of the control. In addition,the content of chlorophyll in C. camphora under different concentrations of cadmium contaminated soils decreased,and chlorophyll in medium and high concentrations of cadmium (Cd2 and Cd3) significantly reduced. The content of proline (Pro),soluble sugar and soluble protein in C. camphora leaves was increased by cadmium pollution of different concentrations,and the content was the highest under high concentration Cd²⁺ stress (Cd3). After 30 days and 60 days,the activity of SOD in medium and high concentration cadmium treatment (Cd2 and Cd3) was significantly higher than that in the control as well as low concentration cadmium stress. During the whole treatment period,POD activity increased with the pollution time and the cadmium concentration. However,the medium and high concentration cadmium stress had a significant effect on the increase of MDA content. Conclusion: The results suggest physiological and biochemical indexes of C. camphora,such as the photosynthesis parameters,osmotic adjustment substances and antioxidant protective enzymes,are closely related to stress time and pollution concentration. But,C. camphora seedlings grow well under different concentrations of cadmium stress,reflecting that C. camphora has strong cadmium tolerance and thus has potential value for remediation of heavy metal contaminated soil.

Key words: Cinnamomum camphora, cadmium stress, photosynthesis, physiological and biochemical characteristics

CLC Number:

S718.43

Jihai Zhou,Kun Cheng,Ruru Gao,Honglang Duan,Haiyan Pu,Zhinong Jin. Photosynthesis and Other Physiological Characteristics of Cinnamomum camphora Seedlings under Cadmium Stress[J]. Scientia Silvae Sinicae, 2020, 56(6): 193-201.

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关系曲线Relation curve	y=k ln(x)+y_asym
处理Treatment	R²	y_asym	95%CI	k	95%CI
CK	0.86	22.09a	21.09，23.10	5.42	4.90，5.40
Cd1	0.80	22.29a	20.97，23.61	6.06	5.34，6.78
Cd2	0.82	22.13a	20.57，23.68	6.31	5.43，7.19
Cd3	0.74	21.58a	20.28，22.89	5.69	5.05，6.33

处理时间 Treatment time/d	处理 Treatment	脯氨酸 Proline content/ (μg·g^-1 Fw)	可溶性糖 Soluble sugar content/ (mg·g^-1Fw)	可溶性蛋白 Soluble protein content/ (mg·g^-1 Fw)
0	CK	49.99±2.88Ab	20.34±1.44Ac	0.53±0.023Aa
	Cd1	51.35±3.34Ab	20.56±1.33Ad	0.55±0.028Aa
	Cd2	47.61±3.41Ab	19.52±1.25Ad	0.51±0.035Ab
	Cd3	50.59±2.77Ac	20.70±1.53Ab	0.53±0.015Abc
15	CK	36.25±0.66Cc	38.39±0.54Cb	0.27±0.016Cb
	Cd1	49.51±2.73Bb	44.69±0.11Bc	0.35±0.014Bb
	Cd2	40.38±1.58Cc	40.39±1.25Cc	0.44±0.065Ab
	Cd3	59.85±0.76Ab	55.04±0.66Aa	0.47±0.102Ac
30	CK	61.48±1.35Ba	47.42±1.73Ba	0.33±0.011Cb
	Cd1	66.66±3.81Ba	49.78±1.60Bb	0.43±0.044Bb
	Cd2	86.84±2.36Aa	50.19±2.11Bb	0.54±0.046Ab
	Cd3	94.93±2.82Aa	59.24±2.25Aa	0.56±0.024Ab
60	CK	20.22±1.63Bd	44.89±1.46Ba	0.51±0.066Ba
	Cd1	23.22±2.01ABc	56.84±2.15Aa	0.56±0.028Ba
	Cd2	21.62±1.81Bd	58.24±1.56Aa	0.76±0.057Aa
	Cd3	27.85±1.14Ad	58.48±1.40Aa	0.72±0.033Aa

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Photosynthesis and Other Physiological Characteristics of Cinnamomum camphora Seedlings under Cadmium Stress

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