褪黑素处理对高节竹笋低温贮藏过程中木质化的影响

doi:10.11707/j.1001-7488.20191205

Abstract

Abstract:

Objectve: This study was designed to investigate the effect of melatonin on lignin formation, scavenging-enzyme activity, and transcription-factor expression in Phyllostachys prominens shoots stored at 4℃. Method: We investigated the changes in the firmness, yellowness and brightness, lignin and cellulose contents, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities, as well as the gene expression of NAC and MYB transcription factors in bamboo shoots of P. prominens during the different storage time (0, 3, 6, 9 and 12 d) under low temperature (4℃) with or without melatonin (1.0 mmol·L^-1) treatment conditions. Result: Compared with the control, the exogenous melatonin slowed down the rate of hardening and yellowing of the shoots, and reduced lignin and cellulose contents, which proved that it effectively retarded shoot lignification. Furthermore, melatonin treatment inhibited PAL and POD activities significantly, while it enhanced SOD, CAT, and APX activities at different storage stages. Additionally, transcription factors of MYB20, MYB63, MYB85, SND2 and VND7 from the NAC and MYB families were up-regulated, MYB42, MYB43, NST1 and KNAST7 were down-regulated during postharvest storage of the bamboo shoots, and melatonin treatment inhibited the expression of MYB20, MYB42 and KNAT7, while induced the expression ofMYB43, MYB63, MYB85 and SND2 to some extent. Conclusion: These results indicate that melatonin treatment can effectively delay the occurrence of lignification in the process of postharvest low-temperature storage of P. prominens shoots. The delaying effect of postharvest bamboo shoot lignification was mainly attributed to reduction of activities of lignin biosynthesis-related enzymes and improvement of antioxidant capacity of bamboo shoots. Moreover, exogenous melatonin may be involved in the transcriptional regulation of the lignification process of bamboo shoots.

Key words: Phyllostachys prominens, melatonin, lignification, bamboo shoots, low temperature, storage

CLC Number:

S714

Jun Lou,Jinwei Suo,Hui Zhang,Lingling Xuan,Yeqing Ying,Lili Song. Effect of Melatonin Treatment on Lignification of Phyllostachys prominens Shoots during Low Temperature Storage[J]. Scientia Silvae Sinicae, 2019, 55(12): 41-49.

Figures/Tables 8

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		转录因子Transcription factors
		MYB20	MYB42	MYB43	MYB63	MYB85	SND2	NST1	VND7	KNAST7
对照组(CK)	木质素Lignin	0.81^**	-0.89^**	-0.85^**	0.31	0.28	0.04	-0.42	0.27	-0.73^**
	纤维素Cellulose	0.67^**	-0.92^**	-0.87^**	0.19	0.19	0.06	-0.43	0.13	-0.83^**
	苯丙氨酸裂解酶PAL	0.99^**	-0.95^*	-0.91^*	0.07	0.08	0.03	-0.43	0.21	-0.84
	过氧化物酶POD	0.58	-0.82	-0.65	-0.17	-0.21	-0.46	0.08	-0.30	-0.59
处理组MT	木质素Lignin	0.86^**	-0.71^**	-0.66^**	0.39	0.66^**	0.42	0.15	-0.03	-0.56^*
	纤维素Cellulose	0.68^**	-0.71^**	-0.72^**	0.24	0.49	0.32	0.28	-0.21	-0.51
	苯丙氨酸裂解酶PAL	0.72	-0.72	-0.86	0.50	0.59	0.48	0.20	-0.12	-0.40
	过氧化物酶POD	0.86	-0.67	-0.72	0.32	0.63	0.31	0.27	-0.23	-0.46

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Effect of Melatonin Treatment on Lignification of Phyllostachys prominens Shoots during Low Temperature Storage

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