板栗雄花序萃取物抗氧化及抑菌效果

doi:10.11707/j.1001-7488.20150517

Abstract

Abstract:

【Objective】In this study, effects of the different extracts of chestnut catkins on antioxidation and antibacterial were investigated to provide a basis for the further development and utilization of the catkins.【Method】Methanol extract of chestnut catkins was further partitioned with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol and four crude extractives were successively obtained. The antioxidant activities of the different extractives were evaluated by two methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability and resistance to the peroxidation of unsaturated fatty acids in egg yolk. The antimicrobial activity was analyzed by agar diffusion and co-culture method. The structural formula and content of ellagic acid (EA) in the extractives were determined by HPLC and NMR. 【Result】1) Extraction rate of chestnut catkins through methanol extractives was 16.6%, and the rates of other four extractives were between 2.6 % to 26.2 % with ethyl acetate extract the highest. 2) All of extractives were able to some degrees to scavenge DPPH radical and yolk lipid peroxide. Among them, ethyl acetate extract possessed similar antioxidation level compared with vitamin C (Vc) and 2,6- tert butyl-4- methyl phenol (BHT). The rates of scavenging DPPH radical and yolk lipid peroxide were 94.4 % and 95.69 % under a concentration of 0.01 mg mL^-1, respectively. 3) Antimicrobial activity of the methanol extract of chestnut catkins was effective to 12 kinds of bacteria. The ethyl acetate extract had the most inhibitory effect to bacteria in different extractives and the minimum inhibitory concentration and the minimum bactericidal concentration against Escherichia coli were 0.98 mg·mL^-1and 1.96 mg·mL^-1, respectively. The content of free ellagic acid was 2.267 mg·g^-1. 【Conclusion】Chestnut catkins contain abundant antioxidant and antibacterial substances. The antioxidant capacity was different among alcohol extracts of chestnut catkins and polar extracts. The ethyl acetate extract has higher antioxidant activity and higher scavenging rate to DPPH radical and lipid peroxides. Various extractives have to different degrees effects on the G⁺and G^-bacteria, and antibacterial activity of ethyl acetate extract has the highest effect among the several extractives.

Key words: chestnut catkin, solvent extraction, antioxidant, antimicrobial, ellagic acid

CLC Number:

S718.43

Bai Hongwei, Cheng Jun, Yang Liu, Jiang Yichen, Cao Qingqin, Zhang Qing, Qin Ling. Antioxidation and Antimicrobial Activities of Extracts Parts of Chestnut Catkins[J]. Scientia Silvae Sinicae, 2015, 51(5): 145-152.

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Antioxidation and Antimicrobial Activities of Extracts Parts of Chestnut Catkins

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